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GB/T 36779-2018: Detection and identification of Fusarium oxysporum f. sp. apii
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| GB/T 36779-2018 | English | 279 |
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Detection and identification of Fusarium oxysporum f. sp. apii
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GB/T 36779-2018
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Basic data | Standard ID | GB/T 36779-2018 (GB/T36779-2018) | | Description (Translated English) | Detection and identification of Fusarium oxysporum f. sp. apii | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,190 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Regulation (derived from) | National Standard Announcement No. 11 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36779-2018: Detection and identification of Fusarium oxysporum f. sp. apii---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Fusarium oxysporum f.sp.apii
ICS 65.020.01
B16
National Standards of People's Republic of China
Method for quarantine and identification of celery wilt pathogen
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang University, Yantai, People's Republic of China
Inspection and Quarantine Bureau.
The main drafters of this standard. Zhang Weidong, Huang Yonghui, Quan Yongbing, Song Fengming, Chi Yuanli, Xu Weifeng, Liao Li, Lin Wei, Li Jinqing, Yu Jindi.
Method for quarantine and identification of celery wilt pathogen
1 Scope
This standard specifies the method for quarantine and identification of celery wilt.
This standard is applicable to the quarantine and identification of seeds, seedlings, plant residues and celery wilt pathogens that may carry celery fusarium.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products
SN/T 2589 Plant Pathogenic Fungus Detection Specification
3 celery wilt pathogen information
Chinese name. celery wilt pathogen
Scientific name. FusariumoxysporumSchlecht.f.sp.apiiSnyderetHansen.1940
Synonym. FusariumbulbigenumCookeetMassee.1887, FusariumorthocerasAppeletWolenw.1910,
FusariumangustumSherb.1915, Fusariumorthocerasvar.apii (NelsonetSherb.) WolenwetRein-
King.1935, Fusariumapii NelsonetSherb.1937, Fusariumapiivar.pallidum NelsonetSherb.1937,
Fusariumoxysporumf.apii (NelsonetSherb.) SnyderetHansen.1940, Fusariumbulbigenum var.
Apii (Nelson
Classification status. KingdomFungi, Deuteromycota, Hyphomycetes, Neurospora
Tuberculariales, Tuberculariaceae, Fusarium.
See Appendix A for additional information on celery wilt.
4 Principle
The symptoms of the celery wilt pathogen, the culture characteristics of the colony, the host range, the molecular biological characteristics, and the pathogenicity measurement results are
The main basis for its identification.
5 instruments, utensils and main reagents
5.1 Instrument
PCR instrument, light incubator, biological microscope (100×~1000×), stereo microscope, tissue grinder, biological safety cabinet, high speed cold
Freeze centrifuge, micro spectrophotometer, electrophoresis instrument, gel imaging system, electronic balance (inductance 0.0001g), pH meter, autoclave.
5.2 Appliances
Alcohol lamps, gauze, beakers, inoculation needles, inoculating rings, petri dishes, conical flasks, cotton plugs, slides, coverslips, aluminum platinum paper, scalpels, anatomy
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