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GB/T 36770-2018: Detection and identification of Australian grapevine viroid
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| GB/T 36770-2018 | English | 279 |
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Detection and identification of Australian grapevine viroid
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GB/T 36770-2018
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Basic data | Standard ID | GB/T 36770-2018 (GB/T36770-2018) | | Description (Translated English) | Detection and identification of Australian grapevine viroid �� | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,133 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36770-2018: Detection and identification of Australian grapevine viroid ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Australian grapevine viroid
ICS 65.020.01
B16
National Standards of People's Republic of China
Australian grape virus quarantine and identification method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization is not responsible for identifying these patents.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. China Academy of Inspection and Quarantine, Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Fujian Provincial Agricultural Science
Institute, Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Deng Congliang, Zhang Yongjiang, Wang Yingchao, Xiang Jun, Zhao Xiaoli, Xie Lixue.
Australian grape virus quarantine and identification method
1 Scope
This standard specifies the quarantine and identification methods for Australian grape virus.
This standard applies to the quarantine and identification of grape propagation materials that may carry Australian grape virus.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Australian grape virus basic information
Scientific name.Australiangrapevineviroid
Abbreviation. AGVd
Synonym. Australian grape virus
Taxonomic status. Pospiviroidae, apple rust, and Apscaviroid
See Appendix A for additional information on Australian grape viruses.
4 Principle of the method
Viral detection is primarily directed to its nucleic acid sequence. The main methods are reverse transcription polymerase chain reaction (RT-PCR) and nucleic acid molecules.
Hybrid.
5 instruments, main equipment and main reagents
5.1 Instrumentation
Electronic balance (sensitivity 0.001g), ordinary balance (sensitivity 0.1g), high-speed refrigerated centrifuge, small instantaneous centrifuge, ordinary refrigerator, super
Low temperature refrigerator (-80 ° C), ice machine, vortex shaker, magnetic stirrer, autoclave, pH meter, PCR instrument, microwave oven, electrophoresis, electricity
Swimming tank, gel analysis imaging system, real-time fluorescence quantitative PCR instrument, hybridization furnace, plastic film sealing machine, UV cross-linking instrument, black box, shaker, constant
Warm water bath, tissue crusher, etc.
5.2 Main equipment
Adjustable pipette (2.5μL, 10μL, 20μL,.200μL, 1000μL, 5000μL), RNase-free tip (10μL,
20μL,.200μL, 1000μL, 5000μL), RNase-free tube (1.5mL, 5mL, 10mL), mortar, pestle, magnetic
Off-shelf, PCR reaction tube (200 μL), real-time fluorescent 96-well reaction plate, hybrid bottle, hybrid membrane, hybrid bag, X-ray film.
5.3 main reagents
Unless otherwise specified, all reagents are of analytical grade, and the experimental water should meet the relevant provisions of GB/T 6682. Ordinary RT-PCR
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