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 GB/T 36778-2018: Detection and identification of Oat mosaic virus
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 Basic data             | Standard ID | GB/T 36778-2018 (GB/T36778-2018) |           | Description (Translated English) | Detection and identification of Oat mosaic virus |           | Sector / Industry | National Standard (Recommended) |           | Classification of Chinese Standard | B16 |           | Classification of International Standard | 65.020.01 |           | Word Count Estimation | 14,150 |           | Date of Issue | 2018-09-17 |           | Date of Implementation | 2019-04-01 |           | Regulation (derived from) | National Standard Announcement No. 11 of 2018 |           | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36778-2018: Detection and identification of Oat mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Oat mosaic virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Oat mosaic virus quarantine and identification method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
 ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization is not responsible for identifying these patents.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. Ningbo Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang Academy of Agricultural Sciences, People's Republic of China
Beijing Entry-Exit Inspection and Quarantine Bureau, Inner Mongolia Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Guo Lixin, Duan Weijun, Liang Xinmiao, Yang Yongsheng, Sun Liying, Wu Wei, Rong Defu.
Oat mosaic virus quarantine and identification method1 ScopeThis standard specifies the serological, molecular biology and immunoelectron microscopy detection and identification methods for oat mosaic virus.
This standard applies to the quarantine and identification of oat mosaic virus on the soil of the Avena plant, seeds and seeds.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 1840 plant virus immunoelectron microscopy detection method
SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products3 oat mosaic virus basic informationScientific name. Oatmosaicvirus.
Synonym. Soil-borneoatmosaicvirus.
Abbreviation. OMV.
Classification status. Pötyviridae, Bymovirus.
See Appendix A for additional information on oat mosaic virus.4 Principle of the methodDouble antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription polymerase chain reaction (RT-PCR) and immunoelectron microscopy
(IEM) testing is the main basis for the development of this standard.5 instruments and main reagents5.1 Instrumentation
Electronic balance (sensitivity 0.001g), ordinary balance (sensitivity 0.1g), high-speed refrigerated centrifuge, small centrifuge, ultra-low temperature refrigerator
(-80 ° C), ice machine, vortex oscillator, magnetic stirrer, autoclave, pH meter, ultra clean bench, PCR instrument, electrophoresis, electrophoresis
Slot, gel analysis imaging system, microplate reader, plate washer, transmission electron microscope.
5.2 main reagent
All reagents are of analytical grade, and the experimental water should meet the relevant provisions of GB/T 6682. DAS-ELISA detection reagents are shown in Appendix B;
See Appendix C for RT-PCR detection reagents; SN/T 1840 for IEM detection reagents.
 
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