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GB/T 36778-2018 English PDF

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GB/T 36778-2018: Detection and identification of Oat mosaic virus
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Basic data

Standard ID GB/T 36778-2018 (GB/T36778-2018)
Description (Translated English) Detection and identification of Oat mosaic virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 14,150
Date of Issue 2018-09-17
Date of Implementation 2019-04-01
Regulation (derived from) National Standard Announcement No. 11 of 2018
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 36778-2018: Detection and identification of Oat mosaic virus

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Detection and identification of Oat mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Oat mosaic virus quarantine and identification method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Ningbo Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang Academy of Agricultural Sciences, People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, Inner Mongolia Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Guo Lixin, Duan Weijun, Liang Xinmiao, Yang Yongsheng, Sun Liying, Wu Wei, Rong Defu. Oat mosaic virus quarantine and identification method

1 Scope

This standard specifies the serological, molecular biology and immunoelectron microscopy detection and identification methods for oat mosaic virus. This standard applies to the quarantine and identification of oat mosaic virus on the soil of the Avena plant, seeds and seeds.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 1840 plant virus immunoelectron microscopy detection method SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 oat mosaic virus basic information

Scientific name. Oatmosaicvirus. Synonym. Soil-borneoatmosaicvirus. Abbreviation. OMV. Classification status. Pötyviridae, Bymovirus. See Appendix A for additional information on oat mosaic virus.

4 Principle of the method

Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription polymerase chain reaction (RT-PCR) and immunoelectron microscopy (IEM) testing is the main basis for the development of this standard.

5 instruments and main reagents

5.1 Instrumentation Electronic balance (sensitivity 0.001g), ordinary balance (sensitivity 0.1g), high-speed refrigerated centrifuge, small centrifuge, ultra-low temperature refrigerator (-80 ° C), ice machine, vortex oscillator, magnetic stirrer, autoclave, pH meter, ultra clean bench, PCR instrument, electrophoresis, electrophoresis Slot, gel analysis imaging system, microplate reader, plate washer, transmission electron microscope. 5.2 main reagent All reagents are of analytical grade, and the experimental water should meet the relevant provisions of GB/T 6682. DAS-ELISA detection reagents are shown in Appendix B; See Appendix C for RT-PCR detection reagents; SN/T 1840 for IEM detection reagents.

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