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GB/T 36775-2018: Detection and identification of Pseudocercospora angolensis Crous & U. Braun
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36775-2018 | English | 279 |
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Detection and identification of Pseudocercospora angolensis Crous & U. Braun
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GB/T 36775-2018
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Basic data | Standard ID | GB/T 36775-2018 (GB/T36775-2018) | | Description (Translated English) | Detection and identification of Pseudocercospora angolensis Crous & U. Braun | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,149 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Regulation (derived from) | National Standard Announcement No. 11 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36775-2018: Detection and identification of Pseudocercospora angolensis Crous & U. Braun---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pseudocercospora angolensis Crous
ICS 65.020.01
B16
National Standards of People's Republic of China
Method for quarantine and identification of citrus spotted bacteria
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Feng Lixia, Hu Xuenan, Zuo Ranling, Wu Mutao, Wang Weifang, Zhao Lirong, Yu Yu.
Method for quarantine and identification of citrus spotted bacteria
1 Scope
This standard specifies the quarantine and identification methods for citrus spotted bacteria in plant quarantine.
This standard applies to the quarantine and identification of citrus spotted bacteria in citrus seedlings and fruits.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 1538.1 Guidelines for the preparation of medium - Part 1
3 Principle of the method
See Appendix A for the classification, distribution and host information of citrus spotted bacteria. This standard uses the pathogen to host the symptoms, morphological characteristics,
The culture characteristics were used as the basis for the main quarantine and identification, and the pathogenicity test was used as the basis for the auxiliary quarantine identification of citrus spotted bacteria.
4 equipment
4.1 Instrumentation
Stereo microscope, biological microscope, ultra-clean workbench, autoclave, electronic balance (sensitivity 0.01g), biological incubator.
4.2 Laboratory equipment
Labels, qualitative filter paper, sample bags, storage bags, hand-held magnifiers, tweezers, scissors, scalpels, alcohol lamps, slides, coverslips, inoculation needles,
Petri dishes, beakers, measuring cylinders, flasks, glass rods, micropipettes (with a range of 2.5 μL to 1000 μL) and pH meter.
5 reagents and media
5.1 Reagents
Anhydrous ethanol, 0.5% sodium hypochlorite (NaOCl) solution, calcium carbonate (CaCO3), sterile double distilled water.
5.2 medium
Potato Dextrose Agar Medium (PDA). Weigh.200 g of potato, 15 g of glucose, and 15 g of agar. Potato peeled and cut into small
After the block, add water and boil for 20min~30min. After filtering the gauze, add 15g agar, dissolve and add 15g of glucose, and make up the deionized water.
1000 mL, the pH of the medium was adjusted to 5.6, and sterilized at 121 ° C for 20 min. Commercialized PDA powder can also be used according to the instructions for use.
Formulated.
V8 medium (V8A). 100mL V8 vegetable juice, calcium carbonate 0.2g, agar powder 150g, deionized water to make up 1000mL, 121 ° C
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