|
US$119.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 36756-2018: General requirements for activity detection of reagent enzymes
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36756-2018 | English | 119 |
Add to Cart
|
3 days [Need to translate]
|
General requirements for activity detection of reagent enzymes
| Valid |
GB/T 36756-2018
|
PDF similar to GB/T 36756-2018
Standard similar to GB/T 36756-2018 GB/T 41907 GB/T 36757 GB/T 36755 Basic data | Standard ID | GB/T 36756-2018 (GB/T36756-2018) | | Description (Translated English) | General requirements for activity detection of reagent enzymes | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,643 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36756-2018: General requirements for activity detection of reagent enzymes---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
General requirements for activity detection of reagent enzymes
ICS 07.080
C27
National Standards of People's Republic of China
General requirements for the determination of enzyme activity
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Content
Foreword I
Introduction II
1 Scope 1
2 Normative references 1
3 Laboratory General Requirements 1
4 General requirements for materials, reagents and reference materials 1
5 General sampling requirements 1
6 General requirements for sample preparation 2
7 Enzyme activity determination method General requirements 2
8 Testing Quality Assurance 2
9 Test Report 3
10 Sample retention 3
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11).
This standard was drafted. Fujian Huacan Pharmaceutical Co., Ltd., Fujian Nansheng Technology Co., Ltd., Fudan University, Shandong University, Angel Yeast
Co., Ltd., Institute of Metrology and Analysis, China Institute of Metrology, Beijing University of Chemical Technology, Fujian Agriculture and Forestry University, China Agricultural University
Xue, Suzhou Beaver Biomedical Engineering Co., Ltd., Shanghai Bo Shi Biomedical Service Center, Suzhou University, Chinese Academy of Sciences, Microbiology Research
Institute, Xiamen Zhishan Biotechnology Co., Ltd., Anhui University, South China University of Technology, Shanghai Baisai Biotechnology Co., Ltd.
The main drafters of this standard. Huang Facan, Zheng Dengzhong, Zhan Xuexiong, Zhao Jing, Zhong Jiang, Chen Xiulan, Yao Wei, Zhang Lili, Quan Can, Chen Jinchun, Liu Bin,
Li Quanhong, Zhang Xiying, Ren Hui, Xing Zhigang, Zhu Li, Liu Wenjun, Li Jing, Song Najie, Li Qingge, Wang Shihua, Huang Faxi, Xiao Yazhong, Song Daxin,
Lin Ying, Liu Pei.
Introduction
The tool enzyme is a system for cleavage, ligation, synthesis (amplification), modification, and the like of biological molecules such as nucleic acids, proteins, sugars, and lipids.
It is used in biomedical and life science related fields such as genetic engineering, enzyme engineering, protein engineering, and cell engineering. Formulating tool enzyme activity
The determination of national standards for general requirements to promote the industrialization of tool enzymes is of great significance for the production and application of tool enzymes.
General requirements for the determination of enzyme activity
1 Scope
This standard specifies the general requirements for laboratory enzyme activity determination, general requirements for material reagents and reference materials, general sampling requirements,
General requirements for sample preparation, general requirements for enzyme activity determination methods, test quality assurance, test reports and sample retention.
This standard applies to the determination of the enzyme activity of the tool in the process of scientific research, production and application.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
General requirements for GB/T 27025 testing and calibration laboratory capabilities
3 General requirements for laboratories
3.1 The laboratory shall comply with the provisions of GB/T 27025.
3.2 The design of the laboratory should be compatible with the testing requirements. There should be sufficient space to avoid confusion and cross-contamination, and there should be sufficient areas.
The field is used for sample disposal, storage of samples, and record keeping.
3.3 If necessary, a special instrument room should be set up to protect the instrument with high sensitivity from static electricity, vibration, electromagnetic waves, moisture and other factors.
4 General requirements for materials, reagents and reference materials
4.1 The experimental consumables, reagents and reference materials should be purchased from the manufacturer or supplier approved by the qualification agency, and if necessary, the supplier
Evaluation; key reagents, if necessary, should be quality checked before use.
4.2 Chemical reagents should meet at least analytical purity requirements; biochemical reagents should be free of hybrid enzyme contamination; primers used should be PCR grade and/or HPLC
Level; probe should be HPLC grade.
Note. PCR is an abbreviation of Polymerase Chain Reaction (PCR). HPLC is high performance liquid chromatography (HighPer-
Abbreviation for formanceLiquidChromatography, HPLC).
4.3 The test water should meet at least the requirements of GB/T 6682 secondary water.
4.4 When preparing the solvent, the name, concentration, preparation time, storage conditions, expiration date and formulator's name should be indicated on the container.
5 General sampling requirements
5.1 The sampling process should ensure that the products and products are not contaminated.
5.2 Sampling equipment should be clean and disinfected if necessary.
5.3 Sampling should be representative. When multi-packaging, the sampling rules of Table 1 should be used.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36756-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36756-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 36756-2018_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 36756-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|