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GB/T 36755-2018 English PDF

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GB/T 36755-2018: BST DNA polymerase
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GB/T 36755-2018English279 Add to Cart 3 days [Need to translate] BST DNA polymerase Valid GB/T 36755-2018

PDF similar to GB/T 36755-2018


Standard similar to GB/T 36755-2018

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Basic data

Standard ID GB/T 36755-2018 (GB/T36755-2018)
Description (Translated English) BST DNA polymerase
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard C27
Classification of International Standard 07.080
Word Count Estimation 14,168
Date of Issue 2018-09-17
Date of Implementation 2019-04-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 36755-2018: BST DNA polymerase

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
BST DNA polymerase ICS 07.080 C27 National Standards of People's Republic of China BSTDNA polymerase Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Content

Foreword III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Technical requirements 1 5 Test method 1 6 Packaging, transportation and storage 2 7 Shelf life 2 Appendix A (Normative Appendix) BST Enzyme Activity Detection 3 Appendix B (Normative) Exonuclease Detection 7 Appendix C (Normative) Endonuclease Detection 9

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11). This standard was drafted. Institute of Microbiology, Chinese Academy of Sciences, Xiamen Zhishan Biotechnology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd. Company, Fujian Nansheng Technology Co., Ltd., Angel Yeast Co., Ltd., Shanghai Bo Shi Biomedical Service Center, Beijing University of Chemical Technology. The main drafters of this standard. Li Jing, Song Najie, Huang Facan, Liu Wenjun, Zhan Xuexiong, Zheng Dengzhong, Li Qingge, Zhao Jing, Zhang Lili, Yao Wei, Xing Zhigang, Chen Jinchun.

Introduction

A portion of DNA polymerase has 5'→3' DNA polymerase activity but does not have 5'→3' exonuclease activity. Develop BST The National Standard for DNA Polymerases, which promotes the industrialization of such tool enzymes, is important for the production and use of BST DNA polymerases. significance. BSTDNA polymerase

1 Scope

This standard specifies the technical requirements, testing methods, packaging, transportation, storage and shelf life of BST DNA polymerase. This standard applies to BST DNA polymerase extracted by recombinant expression.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 191 packaging storage and transportation icon

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Part of the DNA polymerase of Bacillus stearotherxophilus (Bacillus stearothermophilus) with 5'→3' DNA polymerase Active, but not 5'→3' exonuclease activity. 3.2 Using a synthetic hairpin oligonucleotide sequence as a template/primer, 1.29 nmol of deoxynucleotide was polymerized at 65 ° C for 1 min. The amount of enzyme required in double-stranded DNA is 1 viability unit (U).

4 Technical requirements

4.1 Appearance Clarify the clear liquid without precipitation. 4.2 Enzyme activity ≥8000U/mL. 4.3 Impurities Exonuclease and endonuclease should not be included.

5 Test methods

5.1 Appearance Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions.

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