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GB/T 36757-2018: M-MLV reverse transcriptase
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36757-2018 | English | 279 |
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M-MLV reverse transcriptase
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GB/T 36757-2018
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PDF similar to GB/T 36757-2018
Standard similar to GB/T 36757-2018 GB/T 41907 GB/T 36756 GB/T 36755 Basic data | Standard ID | GB/T 36757-2018 (GB/T36757-2018) | | Description (Translated English) | M-MLV reverse transcriptase | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,181 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36757-2018: M-MLV reverse transcriptase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
M-MLV reverse transcriptase
ICS 07.080
C27
National Standards of People's Republic of China
M-MLV reverse transcriptase
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Content
Foreword III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Technical requirements 1
5 Test method 1
6 Packaging, transportation and storage 2
7 Shelf life 2
Appendix A (Normative) M-MLV Reverse Transcriptase Activity Detection 3
Appendix B (Normative) Exonuclease Detection 7
Appendix C (Normative) Endonuclease Detection 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11).
This standard was drafted. Institute of Microbiology, Chinese Academy of Sciences, Xiamen Zhishan Biotechnology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd.
Company, Fujian Nansheng Technology Co., Ltd., Angel Yeast Co., Ltd., Shanghai Bo Shi Biomedical Service Center, Beijing University of Chemical Technology.
The main drafters of this standard. Li Jing, Song Najie, Huang Facan, Liu Wenjun, Zhan Xuexiong, Zheng Dengzhong, Li Qingge, Zhao Jing, Zhang Lili, Yao Wei,
Xing Zhigang, Chen Jinchun.
Introduction
Complementary DNA (cDNA) enzymes for the synthesis of first-strand cDNA, cDNA probes, RNA transcription, sequencing, and RNA reversal
Record the reaction. Develop a national standard for M-MLV reverse transcriptase to promote the industrialization of such tool enzymes for M-MLV reverse transcriptase
Production and use are important.
M-MLV reverse transcriptase
1 Scope
This standard specifies the technical requirements, testing methods, packaging, transportation and storage of M-MLV reverse transcriptase.
This standard applies to M-MLV reverse transcriptase extracted by genetic recombinant expression.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 packaging storage and transportation icon
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
An enzyme that directs the synthesis of complementary DNA (cDNA) by deoxynucleotide triphosphate using RNA as a template.
3.2
Using a synthetic hairpin oligonucleotide sequence as a template/primer, 0.105 nmol of deoxynucleotide at 37 ° C, 18.67 min
The amount of enzyme required to polymerize into double-stranded DNA is 50 viability units.
4 Technical requirements
4.1 Appearance
Clarify the clear liquid without precipitation.
4.2 Enzyme activity
≥200U/μL.
4.3 Impurities
Exonuclease and endonuclease should not be included.
5 Test methods
5.1 Appearance
Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions.
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