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GB/T 36390-2018: Reagent enzyme -- Lysozyme
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36390-2018 | English | 199 |
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Reagent enzyme -- Lysozyme
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GB/T 36390-2018
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PDF similar to GB/T 36390-2018
Standard similar to GB/T 36390-2018 GB/T 41907 GB/T 44828 GB/T 36389 Basic data | Standard ID | GB/T 36390-2018 (GB/T36390-2018) | | Description (Translated English) | Reagent enzyme -- Lysozyme | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,157 | | Date of Issue | 2018-06-07 | | Date of Implementation | 2019-01-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36390-2018: Reagent enzyme -- Lysozyme---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Reagent enzyme--Lysozyme
ICS 07.080
C27
National Standards of People's Republic of China
Tool enzyme lysozyme
Published on.2018-06-07
2019-01-01 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11).
This standard was drafted. Fujian Huacan Pharmaceutical Co., Ltd., Fujian Nansheng Technology Co., Ltd., Beijing University of Chemical Technology, Shandong University, An Qi
Yeast Co., Ltd., Suzhou Beaver Biomedical Engineering Co., Ltd., Institute of Metrology and Analytical Science, China Institute of Metrology.
The main drafters of this standard. Zhan Xuexiong, Zhao Jing, Zheng Dengzhong, Huang Facan, Zhang Lili, Chen Jinchun, Chen Xiulan, Yao Wei, Ren Hui, Quan Can.
Introduction
Lysozyme, also known as cell wall lytic enzyme, is a hydrolase that acts specifically on the cell wall of microorganisms and is capable of hydrolyzing N-acetylmuramic acid and
Β-1,4 glycosidic bond between N-acetylglucosamine. The establishment of national standards for lysozyme is important for promoting the industrialization of such tools.
significance.
Tool enzyme lysozyme
1 Scope
This standard specifies the technical requirements, testing methods, packaging, transportation, storage and shelf life of lysozyme.
This standard applies to lysozyme extracted from biological materials.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 packaging storage and transportation icon
GB/T 30990 lysozyme activity detection method
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Lysozyme lysozyme
Cell wall lyase
A hydrolase that acts specifically on the microbial cell wall and is capable of hydrolyzing β-1,4 between N-acetylmuramic acid and N-acetylglucosamine.
Glycosidic bond.
3.2
Lysozyme activity unit activityunitoflysozyme
Using M. solani as a substrate, 0.1 mol/L phosphate buffer (pH 6.2) as a buffer system, at 25 ° C, 2.5 mL of bacterial suspension and
0.5mL enzyme reaction, the absorbance is measured at a wavelength of 450nm, and the amount of enzyme required to decrease the absorbance value by 0.001 per minute is 1 live.
Sex unit (U).
4 Technical requirements
4.1 Product appearance
White or off-white lyophilized cake or powder.
4.2 Lysozyme activity
≥20000U/mg.
4.3 Impurities
Exonuclease and endonuclease should not be included.
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