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GB/T 36389-2018: T4 DNA ligase
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36389-2018 | English | 199 |
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T4 DNA ligase
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GB/T 36389-2018
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PDF similar to GB/T 36389-2018
Standard similar to GB/T 36389-2018 GB/T 41907 GB/T 44828 GB/T 36390 Basic data | Standard ID | GB/T 36389-2018 (GB/T36389-2018) | | Description (Translated English) | T4 DNA ligase | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,154 | | Date of Issue | 2018-06-07 | | Date of Implementation | 2019-01-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36389-2018: T4 DNA ligase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
T4 DNA ligase
ICS 07.080
C27
National Standards of People's Republic of China
T4 DNA ligase
Published on.2018-06-07
2019-01-01 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11).
This standard was drafted. Fujian Nansheng Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., Suzhou Beaver Biomedical Engineering Co., Ltd.
Division, Xiamen Zhishan Biotechnology Co., Ltd., Xiamen University, Angel Yeast Co., Ltd.
The main drafters of this standard. Zheng Dengzhong, Zhan Xuexiong, Huang Facan, Ren Hui, Zhao Jing, Zhang Lili, Song Najie, Zhang Yongyou, Yao Wei.
Introduction
T4 DNA ligase is derived from recombinant genetically engineered bacteria and catalyzes the phosphodiester of the 5'-P terminus and the 3'-OH terminus of adjacent DNA strands.
The bond-binding reaction promotes the connection between the viscous ends or between the blunt ends. Develop a national standard for T4 DNA ligase to promote this
The industrialization of class tool enzymes is of great significance.
T4 DNA ligase
1 Scope
This standard specifies the technical requirements, testing methods, packaging, transportation, storage and shelf life of T4 DNA ligase.
This standard applies to T4 DNA ligase isolated and purified from recombinant genetic engineering bacteria.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 packaging storage and transportation icon
GB/T 6682 Analytical laboratory water specifications and test methods
YY/T 0087 electrophoresis device
YY/T 0657 medical centrifuge
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
A nucleic acid ligase that catalyzes the reaction of the 5'-P terminal and the 3'-OH terminal of a neighboring DNA strand by a phosphodiester bond
The connection between the sticky ends or between the flat ends.
3.2
In 20 μL of 1×T4 DNA ligase reaction buffer, 50% of HindIII digestion can be achieved in 30 min under 16 °C reaction conditions.
The amount of enzyme required for ligation of the λDNA fragment [5' end concentration of 0.12 μmol/L (300 μg/mL)] was defined as 1 unit of activity (U).
4 Technical requirements
4.1 Appearance
Clarify the clear liquid without precipitation.
4.2 T4DNA ligase activity
≥8000U/mL.
4.3 Impurities
Exonuclease and endonuclease should not be included.
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