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GB/T 44828-2024: Assay method of glucose oxidase activity
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 44828-2024 | English | 169 |
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Assay method of glucose oxidase activity
| Valid |
GB/T 44828-2024
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Standard similar to GB/T 44828-2024 GB/T 51033 GB/T 50082 Basic data | Standard ID | GB/T 44828-2024 (GB/T44828-2024) | | Description (Translated English) | Assay method of glucose oxidase activity | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,877 | | Date of Issue | 2024-10-26 | | Date of Implementation | 2024-10-26 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 44828-2024: Assay method of glucose oxidase activity---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT44828-2024
ICS 07.080
CCSC27
National Standard of the People's Republic of China
Glucose oxidase activity detection method
Released on October 26, 2024
Implementation on October 26, 2024
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Principle 1
5 Reagents or materials 1
6 Test Step 2
7 Quality Control 3
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Tool Enzyme Standardization Working Group (SAC/SWG11).
This document was drafted by. Nansheng (Xiamen) Analysis and Testing Co., Ltd., Centre Testing International Group Co., Ltd., Ningxia Xiasheng Industry
Group Co., Ltd., Weihai Advanced Medical Materials and High-end Medical Devices Shandong Provincial Laboratory, Xiamen Yinxiang Group Co., Ltd., Shenzhen Xinchan
Industrial Biomedical Engineering Co., Ltd., Wuhan Xinhuayang Biological Co., Ltd., Langfang Nordo Zhongke Medical Testing Laboratory Co., Ltd.
Shenzhen Haituo Huaqing Biotechnology Co., Ltd., Tianjin Bofeide Technology Co., Ltd., Guangzhou Jinde Biotechnology Co., Ltd., Shanxi
Dayu Bioengineering Co., Ltd., Hebei Institute of Microbiology Co., Ltd., Wuhan Hanhai New Enzyme Biotechnology Co., Ltd., Shenzhen Hua
Tongwei International Inspection Co., Ltd., Hangzhou Anxu Biotechnology Co., Ltd., Guangdong Jiadele Technology Co., Ltd., Boliduo Biotechnology Co., Ltd.
Technology (Shijiazhuang) Co., Ltd., Xiahe (Shenzhen) Biotechnology Co., Ltd., Xiahe (Hangzhou) Biotechnology Co., Ltd., Fujian Nansheng Technology
Co., Ltd., Fudan University, Shandong University, Soochow University, Xiamen University, Fujian Agriculture and Forestry University, and Xiamen Huaxia College.
The main drafters of this document are. Huang Facan, Zheng Dengzhong, Bai Honghai, Shen Tao, Zhang Zhigang, Ma Fuqiang, Du Kai, Xu Li, Pang Tieliang, He Yi,
Wang Pengyuan, Li Minyou, Zhang Guangmin, Ma Qinghe, Shi Jingni, Yu Liangqing, Chen Dong, Xu Sheng, Zhang Gengbo, Zhao Yi, Guo Yanwei, Lin Yanna, Wang Tianhua,
Zheng Tianye, Huang Enming, Tang Lin, Pan Wei, Zhong Jiang, Chen Xiulan, Zhu Li, Zhang Yongyou, Liu Bin, Zhao Chao, Zhou Zhongai, Pan Paifeng, and Huang Haiyan.
Glucose oxidase activity detection method
1 Scope
This document describes a method for detecting glucose oxidase activity.
This document is applicable to the detection of glucose oxidase activity.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
glucose oxidase
A dehydrogenase that specifically catalyzes the conversion of β-D-glucose to D-gluconic acid and hydrogen peroxide in the presence of oxygen.
3.2
At pH 5.1 and 35°C, 1.0 μmol of β-D-glucose can be oxidized into D-gluconic acid and hydrogen peroxide per minute.
The amount of enzyme required is one activity unit (U).
4 Principles
Under the action of glucose oxidase, glucose reacts with oxygen to produce gluconic acid and hydrogen peroxide, hydrogen peroxide and colorless reduced form.
Under the action of peroxidase, o-dianisidine generates water and red oxidized o-dianisidine, which is a red substance at a wavelength of 500nm.
There is a specific absorption peak, and the number of activity units of the enzyme can be calculated by measuring the rate of increase of the absorbance of the red substance at 500nm.
5 Reagents or materials
5.1 Water
Grade II water in accordance with GB/T 6682.
5.2 50mmol/L sodium acetate buffer
Weigh 0.82g of anhydrous sodium acetate, dissolve it in 150mL of pure water, adjust the pH to 5.1 with 1mol/L hydrochloric acid at 35℃, and dilute to 5.5ml.
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