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GB/T 30945-2014: Determination of tylosin in feeds -- High performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 30945-2014 | English | 169 |
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Determination of tylosin in feeds -- High performance liquid chromatography
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GB/T 30945-2014
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Basic data | Standard ID | GB/T 30945-2014 (GB/T30945-2014) | | Description (Translated English) | Determination of tylosin in feeds -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 7,736 | | Date of Issue | 7/8/2014 | | Date of Implementation | 1/8/2015 | | Quoted Standard | GB/T 6682; GB/T 14699.1; GB/T 20195 | | Regulation (derived from) | 2014 National Standards Bulletin No. 18 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method of high performance liquid chromatography (HPLC) measured feed tylosin. This standard applies to feed, concentrated feed, feed tylosin determination additive premix the standard limit of quantification is 1 mg/kg. This s | GB/T 30945-2014: Determination of tylosin in feeds -- High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of tylosin in feeds. High performance liquid chromatography
ICS 65.120
B46
National Standards of People's Republic of China
Determination of feed tylosin
High Performance Liquid Chromatography
Issued on. 2014-07-08
2015-01-08 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized.
This standard was drafted. Ministry of Agriculture Feed Quality Supervision and Testing Center (Xi'an).
The main drafters. Zhang Mei, Jia Qing, Li Huiling, West Feng Hui, Wang Liang, Guo Jie, Li Sheng.
Determination of feed tylosin
High Performance Liquid Chromatography
1 Scope
This standard specifies the method of high performance liquid chromatography (HPLC) measured feed tylosin.
This standard applies to feed, concentrated feed, feed tylosin determination additive premix, the method limit of quantification
Of 1mg/kg.
This standard does not apply to adding urea feed.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
GB/T 20195 Animal feed sample preparation
Principle 3
After the sample tylosin methylene chloride extraction, filtration, the filtrate was purified by HLB cartridge, and concentrated into high performance liquid chromatography
Line separation, detection with UV detector, external standard.
4 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade reagent water in line with GB/T 6682 in a water regulations.
4.1 methanol.
4.2 acetonitrile. chromatography.
4.3 Methanol. chromatographically pure.
4.4 dichloromethane.
4.5 n-hexane.
4.6 ammonia.
4.7 acetonitrile solution (50%). Take acetonitrile (4.2) was mixed with 100mL 100mL water.
4.8 hydrochloric acid solution (1mol/L). Take 9mL hydrochloride in 100mL flask, diluted with water and set the volume.
4.9 tylosin standard. Purity ≥98%.
4.10 0.005mol/L ammonium dihydrogen phosphate buffer solution. Accurately weigh 0.5751g ammonium dihydrogen phosphate in 1000mL volumetric flask, add water-soluble
Solutions and volume, over 0.45μm filters.
4.11 eluent. 2% methanol aqueous solution of 5% aqueous ammonia, ammonia draw 2mL (4.6), 5mL methanol (4.1), diluted with water
100mL.
4.12 sodium acetate buffer (pH = 5.5,0.15mol/L). Weigh 12.30g of anhydrous sodium acetate in 1000mL volumetric flask, 900mL
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