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GB/T 30955-2014: Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up
Status: Valid
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| GB/T 30955-2014 | English | 169 |
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Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up
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GB/T 30955-2014
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Basic data | Standard ID | GB/T 30955-2014 (GB/T30955-2014) | | Description (Translated English) | Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 7,724 | | Date of Issue | 7/8/2014 | | Date of Implementation | 1/10/2015 | | Quoted Standard | GB/T 6682; GB/T 14699.1; GB/T 20195 | | Regulation (derived from) | 2014 National Standards Bulletin No. 18 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the feed aflatoxin immunoaffinity column clean - HPLC method for measuring. This standard applies to the Determination of aflatoxin in feed. | GB/T 30955-2014: Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up
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Determination of aflatoxin B1, B2, G1, G2 content in feeds. High performance liquid chromatography with immunoaffinity column clean-up
ICS 65.120
B46
National Standards of People's Republic of China
Determination of aflatoxin in feed B1, B2, G1, G2 of
Immunoaffinity column clean - High Performance Liquid Chromatography
DeterminationofaflatoxinB1, B2, G1, G2contentinfeeds-
Issued on. 2014-07-08
2015-01-10 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized.
This standard was drafted. Feed Science Institute, Chinese Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Research Institute, Zhejiang University [State
Home Feed Quality Supervision and Inspection Center (Beijing)], Beijing seized Technology Co., Ltd. Viacom.
The main drafters of this standard. YU Dong, Rao Zhenghua, LI Wei-fen, Li Lan, Wang Xiong, fruit flag, Liang rights.
Determination of aflatoxin in feed B1, B2, G1, G2 of
Immunoaffinity column clean - High Performance Liquid Chromatography
1 Scope
This standard specifies the feed of aflatoxin B1, B2, G1, G2 immunoaffinity column clean - HPLC determination
method.
This standard applies to feed Determination of aflatoxin B1, B2, G1, G2's.
The standard detection limits. aflatoxin B1 was 0.2μg/kg, aflatoxin B2 was 0.2μg/kg, aflatoxin G1 is
0.3μg/kg, aflatoxin G2 is 0.3μg/kg.
The standard limit of quantitation. aflatoxin B1 was 1.0μg/kg, aflatoxin B2 was 1.0μg/kg, aflatoxin G1 is
1.0μg/kg, aflatoxin G2 of 1.0μg/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
GB/T 20195 Animal feed sample preparation
Principle 3
After the sample methanol - after water extraction, the extract was filtered, diluted filtrate containing aflatoxin through specific antibody immunoaffinity layer
Analysis of column chromatography purification, separation by high performance liquid chromatography, fluorescence detection after column photochemical derivatization determination of aflatoxins B1, B2, G1, G2 of
content.
4 Reagents
Unless otherwise indicated, all analytical reagents; laboratory water in line with GB/T 6682 in two water regulations, standards and mobile solution
A water phase in line with the provisions of water.
4.1 Methanol. chromatographically pure.
4.2 Benzene. chromatography.
4.3 acetonitrile. chromatography.
4.4 aqueous methanol (82). Take 80mL of methanol (4.1) add 20mL water, mix well.
4.5 methanol-water (4555). Take 45mL of methanol (4.1) add 55mL water, mix well.
4.6 benzene acetonitrile (982). Take 2mL acetonitrile (4.3) was added 98mL benzene (4.2), mix well.
4.7 aflatoxin standard stock solution. benzene acetonitrile (982) solution (4.6) were prepared 0.100mg/mL aflatoxin
B1, B2, G1, G2 standard stock solution and stored at 4 ℃ spare, you can use for 1 year.
4.8 aflatoxin mixed standard working solution. accurate Pipette the appropriate amount of aflatoxin B1, B2, G1, G2 standard stock solution (4.7),
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