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GB/T 26348-2010: Test methods of mosquito resistance to insecticides -- Test methods of insensitive acetylcholinesterase for the mosquito
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26348-2010 | English | 169 |
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Test methods of mosquito resistance to insecticides -- Test methods of insensitive acetylcholinesterase for the mosquito
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GB/T 26348-2010
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Basic data | Standard ID | GB/T 26348-2010 (GB/T26348-2010) | | Description (Translated English) | Test methods of mosquito resistance to insecticides -- Test methods of insensitive acetylcholinesterase for the mosquito | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C05 | | Classification of International Standard | 11.020 | | Word Count Estimation | 7,70 | | Date of Issue | 2011-01-14 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2011 No.126 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the mosquito body insensitive acetylcholinesterase (ACNE) detection method. This standard applies to Culex mosquito (Culex pipiens PaLens), Culex quinquefasciatus (Cx. pipiens quinquefasciatus), Aedes albopictus (Aedes albopictus), Anopheles (Anopheles sinensis) and other organic phosphorus, carbamates biochemical detection of insecticide resistance. | GB/T 26348-2010: Test methods of mosquito resistance to insecticides -- Test methods of insensitive acetylcholinesterase for the mosquito
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test methods of mosquito resistance to insecticides. Test methods of insensitive acetylcholinesterase for the mosquito
ICS 11.020
C05
National Standards of People's Republic of China
Mosquito resistance detection method
Insensitive acetylcholinesterase law
Issued on. 2011-01-14
2011-05-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard was drafted. People's Liberation Army Institute of Microbiology and Epidemiology, Military Medical Sciences, China Agricultural University, Beijing disease
Disease Prevention and Control Center.
The main drafters of this standard. Zhao Tong Yan, Wang Zhongming, Li Chunxiao, Germany Dong Yan, GAO Xi Wu, Zeng Peng, Ying Tong.
Mosquito resistance detection method
Insensitive acetylcholinesterase law
1 Scope
This standard specifies the mosquitoes insensitive acetylcholinesterase (AChE) detection method.
This standard applies to Culex pipiens (Culexpipienspallens), Culex quinquefasciatus (Cx.pipiensquinquefasciatus), Aedes
Mosquito (Aedesalbopictus), Anopheles (Anophelessinensis) and other organophosphate, carbamate insecticides resistant organisms
Chemical detection.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Acetylcholinesterase distinction concentration discriminatingconcentrationofacetylcholinesterase
It can inhibit the mosquito acetylcholinesterase sensitive physiologically active concentration of 90% insecticide.
3 test insects
Multi-site collection larvae kept indoors, using post-emergence 3d ~ 5d not blood-sucking female mosquitoes were detected under ice-cooling for storage of up to
1d, at -20 ℃ storage conditions to store no more than six months within 1 week or -80 ℃ condition.
4 Reagents
4.1 disodium hydrogen phosphate (Na2HPO4), AR.
4.2 potassium dihydrogen phosphate (KH2PO4), AR.
4.3 pull-through X-100 (TritonX-100).
4.4 acetylthiocholine (ATCh), AR.
4.5 5,5'-dithiobis-nitrobenzoic acid (DTNB), AR.
4.6 phosphate buffer (0.1mol/L, pH7.5PBS). Weigh 30.18gNa2HPO4 · 12H2O and 2.16gKH2PO4, distillation
Water volume to 1000mL.
4.7 DTNB- reagent ethanol. 12.4mgDTNB, plus 120mL95% ethanol, 80mL of distilled water, 50mL phosphate buffer
(PH7.5,0.1mol/L, containing 0.1% TritonX-100).
4.8 ATCh solution. Weigh 28.9mgATCh dissolved in 10mL distilled water, -20 ℃ stored.
4.9 ATCh-DTNB mixed solution. Weigh 75mgATCh and 13mgDTNB, dissolved in 100mL phosphate buffer.
5 spectrophotometry
5.1 Equipment
5.1.1 spectrophotometer.
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