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GB/T 26349-2010: Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26349-2010 | English | 169 |
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Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica
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GB/T 26349-2010
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Basic data | Standard ID | GB/T 26349-2010 (GB/T26349-2010) | | Description (Translated English) | Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C05 | | Classification of International Standard | 11.020 | | Word Count Estimation | 7,715 | | Date of Issue | 2011-01-14 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2011 No.127 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the housefly (Musca domestica) insensitive acetylcholinesterase (ACNE) detection method. This standard applies to houseflies to organophosphate, biochemical detection of carbamate insecticides resistance. | GB/T 26349-2010: Test methods of fly resistance to insecticides -- Test methods of insensitive acetylcholinesterase for musca domestica
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Test methods of fly resistance to insecticides. Test methods of insensitive acetylcholinesterase for musca domestica
ICS 11.020
C05
National Standards of People's Republic of China
Flies resistance detection method
Housefly insensitive acetylcholinesterase law
Issued on. 2011-01-14
2011-05-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given in drafting the rules.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard was drafted. China Agricultural University, Beijing Center for Disease Control and Prevention, the PLA Military Academy of Medical Sciences microorganisms
Institute of Epidemiology matter.
The main drafters of this standard. GAO Xi Wu, Zeng Peng, Pei Liang, Ma Yan, Ting, Tong Ying, Zhao Tong statement.
Flies resistance detection method
Housefly insensitive acetylcholinesterase law
1 Scope
This standard specifies the housefly (Muscadomestica) insensitive acetylcholinesterase (AChE) detection method.
This standard applies to housefly biochemical detection of organophosphate, carbamate insecticides resistant.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Acetylcholinesterase distinction concentration discriminatingconcentrationofacetylcholinesterase
Concentration sensor capable of inhibiting AChE of Musca domestica 90% of the normal activity of the insecticide, in units of mol/L or
mmol/L.
2.2
F1 generation F1generation
From the scene captured housefly breeding to get the first generation.
3 test insects
Housefly site collection, kept indoors, after the emergence of F1's female houseflies 3d ~ 5d. Under ice storage of up to 1d,
-20 ℃ storage conditions at no more than 1 week or -80 ℃ storage conditions within six months.
4 Reagents
4.1 Disodium hydrogen phosphate (Na2HPO4), chemically pure.
4.2 potassium dihydrogen phosphate (KH2PO4), chemically pure.
4.3 pull-through X-100 (TritonX-100).
4.4 acetylthiocholine (ATCh), AR.
4.5 5,5'-dithiobis-nitrobenzoic acid (DTNB), AR.
4.6 phosphate buffer (0.1mol/L, pH7.5PBS). Weigh 30.18gNa2HPO4 · 12H2O and 2.16gKH2PO4, distillation
Water volume to 1000mL.
4.7 DTNB- ethanol reagent. Weigh 12.4mgDTNB, dissolved 125mL95% ethanol, 50mL phosphate buffer, most
After the distilled water volume to 250mL, 4 ℃ stored.
4.8 ATCh solution. Weigh 28.9mgATCh dissolved in 10mL distilled water, -20 ℃ stored.
5 spectrophotometry
5.1 Equipment
5.1.1 spectrophotometer.
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