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GB/T 26347-2010: Test methods of mosquito resistance to insecticides -- Bioassay methods
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 26347-2010 | English | 209 |
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Test methods of mosquito resistance to insecticides -- Bioassay methods
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GB/T 26347-2010
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Basic data | Standard ID | GB/T 26347-2010 (GB/T26347-2010) | | Description (Translated English) | Test methods of mosquito resistance to insecticides -- Bioassay methods | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C05 | | Classification of International Standard | 11.020 | | Word Count Estimation | 9,999 | | Date of Issue | 2011-01-14 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2011 No.2 (Total No.167) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the determination of organic phosphorus mosquitoes, carbamate, pyrethroid insecticides such as Hangzhou potency and sensitive diagnostic dose method baseline method. This standard applies to Culex mosquito (Culex pipiens PaLens), Culex quinquefasciatus (Cx. pipiens quinquefasciatus), Aedes albopictus (Aedes albopictus), Anopheles (Anopheles sinensis) and other mosquito resistance bioassay. | GB/T 26347-2010: Test methods of mosquito resistance to insecticides -- Bioassay methods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test methods of mosquito resistance to insecticides. Bioassay methods
ICS 11.020
C05
National Standards of People's Republic of China
Mosquito resistance detection method bioassay
Issued on. 2011-01-14
2011-05-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard was drafted. People's Liberation Army Institute of Microbiology and Epidemiology, Military Medical Sciences, China Agricultural University, Beijing disease
Prevention and Control Center.
The main drafters of this standard. Zhao Tong Yan, Wang Zhongming, Germany Dong Yan, Zeng Peng, GAO Xi Wu, Song Lin Feng, Tong Ying, Han Yuhua.
Mosquito resistance detection method bioassay
1 Scope
This standard specifies the determination mosquito organophosphorus, carbamates, pyrethroids and other insecticides resistance diagnostic dose method
And sensitive baseline method.
This standard applies to Culex pipiens (Culexpipienspallens), Culex quinquefasciatus (Cx.pipiensquinquefasciatus), Aedes
Mosquito (Aedesalbopictus), Anopheles (Anophelessinensis) resistance of mosquitoes and other biometrics.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Sensitive baseline susceptiblebaselinedata
The measured mosquitoes susceptible strains of certain pesticides virulence regression line (LD-p).
2.2
Diagnostic dose diagnosticdose
Baseline calculated by the sensitive strain was sensitive and mortality 99%, or 99.9% of insecticides corresponding dose values \u200b\u200b(LC99 or
LC99.9), generally two times the difference between the value of LC99 or LC99.9 resistant and susceptible populations.
2.3
F1 generation F1generation
The first generation of mosquito samples collected after breeding.
3 test insects
Multi-sample collection.
Larvae dipping method using the F1 generation of fourth instar larvae.
After contact with adult mosquitoes tube method using wild-collected larvae or pupae, 3d ~ 5d not sucking female mosquitoes.
4 Reagents
Acetone, ether, white oils, pesticides original drugs.
5 Instruments
Mosquito cage, filter paper, the contact cylinder (see Appendix A), suction mosquito.
6 Test Conditions
Temperature. 25 ℃ ± 1 ℃, photoperiod. 14L.10D, Relative Humidity. 60% to 80%.
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