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GB/T 24800.8-2009 English PDF

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GB/T 24800.8-2009: Determination of methotrexate in cosmetics by high performance liquid chromatography method
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GB/T 24800.8-2009English189 Add to Cart 3 days [Need to translate] Determination of methotrexate in cosmetics by high performance liquid chromatography method Valid GB/T 24800.8-2009

PDF similar to GB/T 24800.8-2009


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Basic data

Standard ID GB/T 24800.8-2009 (GB/T24800.8-2009)
Description (Translated English) Determination of methotrexate in cosmetics by high performance liquid chromatography method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 8,854
Date of Issue 2009-11-30
Date of Implementation 2010-05-01
Regulation (derived from) National Standard Approval Announcement 2009 No.14 (Total No.154)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the method for the determination of methotrexate in cosmetics. This standard applies to the determination of methotrexate in cosmetics.

GB/T 24800.8-2009: Determination of methotrexate in cosmetics by high performance liquid chromatography method


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Determination of methotrexate in cosmetics by high performance liquid chromatography method ICS 71.100.70 Y42 National Standards of People's Republic of China Determination of methotrexate in cosmetics High Performance Liquid Chromatography Posted 2009-11-30 2010-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by China National Light Industry Council. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard is drafted by. China Inspection and Quarantine Science Research Institute, Shanghai Daily Chemical Industry Research Institute, Shanghai Institute of spices. The main drafters of this standard. Wang Xing, Wu Ting, Zhang, Xiao Haiqing, Xi Guangcheng, Ma Qiang, Shen Min, Kang Wei.

Introduction

The standard test substance is banned substances of "Hygienic Standard for Cosmetics" shall consist of not as a cosmetic raw material, component Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should follow the "Cosmetics Health standards "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health. At present, China has not yet specified the limit value of the substance, of the standards, only cosmetics assay provides a method to detect the substance. Determination of methotrexate in cosmetics High Performance Liquid Chromatography

1 Scope

This standard specifies the method for determination of methotrexate in cosmetics. This standard applies to the determination of methotrexate in cosmetics. The standard for methotrexate detection limit of 1mg/kg, the limit of quantification of 2.5mg/kg. Principle 2 Mobile phase - methanol 0.025mol/L sodium dihydrogen phosphate (pH5.0) (30 + 70, v/v) as solvent, ultrasonic extraction, centrifugation, 0.45μm organic membrane filter, the solution was injected with a diode array detector (DAD) liquid chromatography detection, external standard.

3 Reagents and materials

Unless otherwise specified, reagents were of analytical grade. Water for ultra-pure water. 3.1 Methanol. chromatographically pure. 3.2 tetrahydrofuran. chromatography. 3.3 methotrexate, purity of not less than 97%, CAS number. 59-05-2. 3.4 methotrexate standard stock solution. Weigh accurately methotrexate 0.05g, accurate to 0.0001g, in 50mL beaker, add 1mL 0.1mol/L sodium hydroxide solution and dissolved transferred 100mL flask with ultrapure water to volume, that was the concentration of methyl purine ammonia solution As the standard stock solution 500mg/L of. Stock solution chilled in the refrigerator, you can use two months. 3.5 Standard working solution. water above standard stock solution (3.4) respectively dubbed a series of concentration 0.05mg/L, 0.1mg/L, 1mg/L, 2mg/L, 5mg/L, 10mg/L, 20mg/L of standard working solution, stored in the refrigerator can be used for one week. 3.6 0.1mol/L sodium hydroxide solution. Weigh sodium hydroxide 0.4g, accurate to 0.001g, in 50mL beaker, dissolved in water Transferred to a 100mL volumetric flask, add water to volume, that was 0.1mol/L sodium hydroxide solution. 3.7 0.025mol/L of sodium dihydrogen phosphate (pH5.0). Weigh sodium dihydrogen phosphate (NaH2PO4 · 2H2O) 3.9g, accurate to 0.001g, in 100mL beaker, dissolved in water is transferred to 1000mL volumetric flask, add water to volume, that was 0.025mol/L Solution of sodium dihydrogen phosphate, with 0.1mol/L sodium hydroxide solution (3.6) adjusted to pH 5.0.

4 Instrument

4.1 liquid chromatograph equipped with a diode array detector. 4.2 micro-injector, 10μL. 4.3 ultrasonic cleaner. 4.4 Centrifuge, greater than 5000r/min. 4.5 and 0.45μm organic solvent filter filtration membranes. 4.6 plug colorimetric tube, 10mL.

5 measuring step

5.1 Sample Preparation Weigh cosmetic samples about 0.2g, accurate to 0.001g, with plug in 10mL colorimetric tube, add 8mL mobile phase (methanol.

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