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GB/T 24800.1-2009: Determination of 9 tetracyclines in cosmetics by high performance liquid chromatography method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 24800.1-2009 | English | 189 |
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Determination of 9 tetracyclines in cosmetics by high performance liquid chromatography method
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GB/T 24800.1-2009
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Basic data | Standard ID | GB/T 24800.1-2009 (GB/T24800.1-2009) | | Description (Translated English) | Determination of 9 tetracyclines in cosmetics by high performance liquid chromatography method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 8,891 | | Date of Issue | 2009-11-30 | | Date of Implementation | 2010-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2009 No.14 (Total No.154) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for the determination of nine tetracycline antibiotics in cosmetics by high performance liquid chromatography. This standard applies to the determination of skin care cosmetics nine tetracycline antibiotics. | GB/T 24800.1-2009: Determination of 9 tetracyclines in cosmetics by high performance liquid chromatography method
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Determination of 9 tetracyclines in cosmetics by hight performance liquid chromatography method
ICS 71.100.70
Y42
National Standards of People's Republic of China
Determination of nine cosmetic tetracycline antibiotics
High Performance Liquid Chromatography
Posted 2009-11-30
2010-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is an informative annex.
The standard proposed by China National Light Industry Council.
This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized.
This standard is drafted by. China Inspection and Quarantine Science Research Institute, Shanghai Daily Chemical Industry Research Institute, Shanghai Institute of spices.
The main drafters of this standard. Wu Ting, Wang Chao, Ma Qiang, Zhang, Xi Guangcheng, Xiao Haiqing, Li Qiong, Cui Jian Jie.
Introduction
The standard test substance is banned substances of "Hygienic Standard for Cosmetics" shall consist of not as a cosmetic raw material, component
Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should follow the "Cosmetics
Health standards "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health.
At present, China has not yet specified the substance of limited value, the present standards, the determination of these substances in cosmetics only provide detection methods.
Determination of nine cosmetic tetracycline antibiotics
High Performance Liquid Chromatography
1 Scope
This standard specifies a high performance liquid chromatographic method cosmetics of nine tetracycline antibiotics.
This standard applies to the determination of skin care cosmetics nine kinds of tetracycline antibiotics.
This standard minocycline detection limit of 25mg/kg, oxytetracycline, tetracycline, Demeclocycline detection limit of 10mg/kg,
Chlortetracycline, Metacycline, doxycycline detection limit of 5mg/kg, detectable difference to dehydration and dehydration tetracycline tetracycline limit
2.5mg/kg.
Minocycline quantification limit of 50mg/kg, oxytetracycline, tetracycline, Demeclocycline quantification limit of 25mg/kg, gold mildew
Su, Metacycline, doxycycline quantification limit of 10mg/kg, the difference between the dewatering and dehydration tetracycline tetracycline quantification limit of 5mg/kg.
Principle 2
Methanol as solvent, ultrasonic extraction, centrifugation, 0.45μm organic membrane filter, the solution was injected with a diode array detector
(DAD) liquid chromatography detection, external standard.
3 Reagents and materials
Unless otherwise specified, reagents were of analytical grade.
3.1 Methanol. chromatographically pure.
3.2 acetonitrile. chromatography.
3.3 nine kinds of tetracycline, purity of not less than 97.0%; oxytetracycline, purity of not less than 97.9%; chlortetracycline, purity of not less than 99.0%; Dorsey
Prostacyclin, purity of not less than 96.5%; Metacycline, purity of not less than 99.6%; Demeclocycline, purity of not less than 99.0%; dimethylamine four
Prostacyclin, a purity of not less than 99.0%; dehydration tetracycline, purity of not less than 90.0%; difference to dehydration tetracycline, purity of not less than 90.0%.
3.4 nine kinds of tetracyclines standard stock solution. Weigh accurately all types of tetracycline (3.3) 0.1g, accurate to 0.0001g, were placed
50mL beaker, it adds appropriate amount of methanol dissolved quantitatively solution into 100mL volumetric flask, diluted with methanol to the mark, mix, that was a concentration
To 1000mg/L standard stock solution.
Nine kinds of tetracyclines mixed standard stock solution. Pipette were above standard stock solution (3.4) each 10mL to 100mL volumetric flask
With methanol to volume, that was a concentration of 100mg/L mixed standard stock solution.
3.5 nine kinds of tetracyclines standard solution. methanol the mixed standard stock solution (3.5) respectively dubbed a series of concentration
1mg/L, 2mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L of standard working solution, stored in the refrigerator can be used for one week.
3.6 0.01mol/L oxalic acid solution. Weigh oxalic acid (C2H2O4 · 2H2O) 1.26g, accurate to 0.001g, in 50mL beaker, add
Dissolved in water and transferred to 1000mL volumetric flask with water to volume, mix, that was 0.01mol/L of oxalic acid solution.
4 Instrument
4.1 liquid chromatograph equipped with a diode array detector.
4.2 micro-injector, 10μL.
4.3 ultrasonic cleaner.
4.4 Centrifuge, greater than 5000r/min.
4.5 and 0.45μm organic solvent filter filtration membranes.
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