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GB/T 24800.5-2009 English PDF

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GB/T 24800.5-2009: Determination of nitrofurantoin and furazolidone in cosmetics by high performance liquid chromatography method
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GB/T 24800.5-2009English189 Add to Cart 3 days [Need to translate] Determination of nitrofurantoin and furazolidone in cosmetics by high performance liquid chromatography method Valid GB/T 24800.5-2009

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Basic data

Standard ID GB/T 24800.5-2009 (GB/T24800.5-2009)
Description (Translated English) Determination of nitrofurantoin and furazolidone in cosmetics by high performance liquid chromatography method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 8,848
Date of Issue 2009-11-30
Date of Implementation 2010-05-01
Regulation (derived from) National Standard Approval Announcement 2009 No.14 (Total No.154)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the nitrofurantoin and high performance liquid chromatographic method furazolidone in cosmetics. This standard applies to cosmetics nitrofurantoin and furazolidone determined. This standard for nitrofurantoin and furazolidone detection limit of 2mg/kg, the limit of quantification for the 8mg/kg.

GB/T 24800.5-2009: Determination of nitrofurantoin and furazolidone in cosmetics by high performance liquid chromatography method


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Determination of nitrofurantoin and furazolidone in cosmetics by high performance liquid chromatography method ICS 71.100.70 Y42 National Standards of People's Republic of China Cosmetics measured by nitrofurantoin and furazolidone High Performance Liquid Chromatography Posted 2009-11-30 2010-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by China National Light Industry Council. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard was drafted. Chinese Academy of Inspection and Quarantine, the Shanghai Daily Chemical Industry Research Institute, Shanghai Institute of spices. The main drafters. Zhang, Xiao Haiqing, Wu Ting, Guang Xi Cheng, Wang Chao, Wang Xing, Cui Jian Jie Kang Wei.

Introduction

The standard of the test substance is banned substances "Hygienic Standard for Cosmetics" provisions can not be added to the make-up as a cosmetic ingredient In the product. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should meet the "Hygienic Standard for Cosmetics" General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health. At present, China has not yet specified the substance of limited value, the present standards, the determination of these substances in cosmetics only provide detection methods. Cosmetics measured by nitrofurantoin and furazolidone High Performance Liquid Chromatography

1 Scope

This standard specifies the nitrofurantoin and HPLC method furazolidone in cosmetics. This standard applies to cosmetics nitrofurantoin and furazolidone determination. The standard for nitrofurantoin and furazolidone detection limit of 2mg/kg, the limit of quantification for the 8mg/kg. Principle 2 Acetonitrile - methanol (1 + 1, v/v) mixed solution of the solvent extraction, ultrasonic extraction, centrifugation, dried organic 0.45μm membrane filter, the solution Injected with a diode array detector liquid chromatography detection, external standard.

3 Reagents and materials

Unless otherwise specified, reagents were of analytical grade. 3.1 acetonitrile. chromatography. 3.2 Methanol. chromatographically pure. 3.3 nitrofurantoin. purity of not less than 99%. 3.4 furazolidone. purity of not less than 99%. 3.5 acetic acid. 3.6 Extraction solvent. acetonitrile (3.1). methanol (3.2) the volume ratio of (1 + 1, v/v). 3.7 0.4% acetic acid solution. Pipette 2mL glacial acetic acid (3.5), dissolved in water after the transfer to a 500mL volumetric flask, constant volume. 3.8 mobile phase. acetonitrile (3.1). 0.4% acetic acid solution (3.7) by weight (30 + 70, v/v). 3.9 standard stock solution (200μg/mL). Weigh accurately respectively nitrofurantoin (3.3) and furazolidone (3.4) 0.02g, accurate to 0.0001g, In 50mL beaker, add appropriate amount of acetonitrile was dissolved moved 100mL brown volumetric flask with acetonitrile to volume, at 4 ℃ dark, sealed, insurable Save more than three months. 3.10 standard solution. take a certain amount of stock solution (3.9), phase (3.8) diluted to brown volumetric flask with mobile, preparation of a concentration 0.2μg/mL, 0.5μg/mL, 1.0μg/mL, 5μg/mL, 10μg/mL, a solution of 20μg/mL of. 3.11 pore size of 0.45μm organic filtration membrane. Note. Due to nitrofurantoin and furazolidone highly sensitive to light, see light easily decomposed metamorphic, and thus preparation of standard solution must use brown volumetric flask, other Also in need of treatment dark glassware, all the operations should be carried out in the absence of light. Operation should avoid inhalation and exposure to toxic standards Reagents.

4 Instrument

4.1 liquid chromatograph equipped with a diode array detector. 4.2 micro-injector, 10μL. 4.3 ultrasonic cleaner. 4.4 Centrifuge, speed not less than 5000r/min. 4.5 solvent filter pore size can be placed in the organic filtration membrane 0.45μm. 4.6 plug cuvette 25mL.

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