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GB/T 24800.4-2009 English PDF

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GB/T 24800.4-2009: Determination of chlortalidone and phenothiazine in cosmetics by high performance liquid chromatography method
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GB/T 24800.4-2009English189 Add to Cart 3 days [Need to translate] Determination of chlortalidone and phenothiazine in cosmetics by high performance liquid chromatography method Valid GB/T 24800.4-2009

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Basic data

Standard ID GB/T 24800.4-2009 (GB/T24800.4-2009)
Description (Translated English) Determination of chlortalidone and phenothiazine in cosmetics by high performance liquid chromatography method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 8,85
Date of Issue 2009-11-30
Date of Implementation 2010-05-01
Regulation (derived from) National Standard Approval Announcement 2009 No.14 (Total No.154)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the HPLC determination of chlorthalidone in cosmetics and phenothiazine. This standard applies to the determination of chlorthalidone in cosmetics and phenothiazines content. This standard for chlorthalidone and phenothiazines detection limit of 2mg/kg, the limit of quantification for the 8mg/kg.

GB/T 24800.4-2009: Determination of chlortalidone and phenothiazine in cosmetics by high performance liquid chromatography method


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Determination of chlortalidone and phenothiazine in cosmetics by high performance liquid chromatography method ICS 71.100.70 Y42 National Standards of People's Republic of China Determination of cosmetics chlorthalidone and phenothiazine High Performance Liquid Chromatography Posted 2009-11-30 2010-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by China National Light Industry Council. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard was drafted. Chinese Academy of Inspection and Quarantine, the Shanghai Daily Chemical Industry Research Institute, Shanghai Institute of spices. The main drafters of this standard. Xiao Haiqing, Zhang, Wang Chao, Wu Ting, Guang Xi Cheng, Wang Xing, Wu Ying, Kang Wei.

Introduction

The standard test substance is banned substances of "Hygienic Standard for Cosmetics" shall consist of not as a cosmetic raw material, component Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should follow the "Cosmetics Health standards "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health. At present, China has not yet specified the substance of limited value, the present standards, the determination of these substances in cosmetics only provide detection methods. Determination of cosmetics chlorthalidone and phenothiazine High Performance Liquid Chromatography

1 Scope

This standard specifies the method for determination by high performance liquid cosmetics chlorthalidone and phenothiazine. This standard applies to the determination of cosmetics chlorthalidone and phenothiazine content. The standard for chlorthalidone and phenothiazines detection limit of 2mg/kg, the limit of quantification for the 8mg/kg. Principle 2 After acetone as solvent extraction, ultrasonic water extraction, centrifugation, organic 0.45μm membrane filter, take 20μL solution was injected with There are UV detector performance liquid chromatography detection, external standard.

3 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade, high pure water. 3.1 acetone. 3.2 Anhydrous sodium dihydrogen phosphate. 3.3 sodium hydroxide. 3.4 Methanol (HPLC grade). 3.5 chlorthalidone, purity of not less than 98.0%. 3.6 phenothiazine, a purity of not less than 99.0%. 3.7 0.5% sodium hydroxide solution. Weigh 2.5g of sodium hydroxide (3.3), to the nearest 0.001g, dissolved in 500mL of water, stored in plastic Bottle. 3.8 30mmol/L sodium dihydrogen phosphate solution. Weigh 3.60g of anhydrous sodium dihydrogen phosphate (3.2), to the nearest 0.001g, added 1000mL Dissolved in water, with 0.5% sodium hydroxide solution (3.7) adjusted to pH 5.60, over the organic membrane 0.45μm. 3.9 Weigh chlorthalidone (3.5) and phenothiazine (3.6) 0.1g, accurate to 0.0001g, dissolved in methanol (3.4), respectively, set the volume to 100mL flask, sealed, dark, cold storage, shelf life of six months. 3.10 chlorthalidone and phenothiazines mixed standard stock solution (200mg/L). Pipette respectively chlorthalidone and phenothiazines standard stock solution (3.9) 10mL In 50mL volumetric flask with methanol (3.4) constant volume, sealed, dark, cold storage, shelf life of six months. 3.11 standard solution. take a certain amount of mixed standard stock solution (3.10), with methanol (3.4) and 30mmol/L sodium dihydrogen phosphate solution (3.8) (55 + 45, v/v), and diluting the concentration of 0.2mg/L, 0.5mg/L, 2mg/L, 5mg/L, 20mg L solution/active Now equipped.

4 Instrument

4.1 high performance liquid chromatography with UV detection. 4.2 micro-injector, 50μL. 4.3 ultrasonic cleaner. 4.4 centrifuge, the maximum speed of not less than 15000r/min. 4.5 solvent filter pore size can be placed in the organic filtration membrane 0.45μm. 4.6 plug colorimetric tube 10mL, 25mL.

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