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GB/T 24800.2-2009 English PDF

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GB/T 24800.2-2009: Determination of 41 glucocorticoids in cosmetics by LC-MS-MS and TLC method
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Basic data

Standard ID GB/T 24800.2-2009 (GB/T24800.2-2009)
Description (Translated English) Determination of 41 glucocorticoids in cosmetics by LC-MS-MS and TLC method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 20,280
Date of Issue 2009-11-30
Date of Implementation 2010-05-01
Quoted Standard GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2009 No.14 (Total No.154)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard provides 41 kinds of cosmetics glucocorticoids liquid chromatography/tandem mass spectrometry and thin layer chromatography two kinds of measuring methods. This standard liquid chromatography/tandem mass spectrometry method is suitable for the quantitative determination of glucocorticoids in cosmetics. The detection limit was 0. 03��g/g, limit of quantification was 0. 1��g/g. This standard method is suitable for cosmetic TLC glucocorticoid qualitative screening. Spotting amount of 10mg, its detection limit of 50��g/g, the amount of time spotting 20mg, its detection limit of 25��g/g.

GB/T 24800.2-2009: Determination of 41 glucocorticoids in cosmetics by LC-MS-MS and TLC method

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Determination of 41 glucocorticoids in cosmetics by LC-MS-MS and TLC method ICS 71.100.70 Y42 National Standards of People's Republic of China Cosmetics forty-one kinds of glucocorticoids Determination Liquid chromatography/tandem mass spectrometry and thin layer chromatography Posted 2009-11-30 2010-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard Annex A, Annex B and Annex C is informative appendix. The standard proposed by China National Light Industry Council. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard was drafted. Dalian City Product Quality Supervision, Inspection, Dalian standard detection technology research center, the national daily chemical products quality supervision Governor Inspection Center. The main drafters of this standard. Pan Wei, Li Peng, Maoxi Qin, Wang Chunyan, Zheng Shunli, Guan Cheng, Yu Lijun, Dongguang Bin.

Introduction

The standard test substance is banned substances of "Hygienic Standard for Cosmetics" shall consist of not as a cosmetic raw material, component Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should follow the "Cosmetics Health standards "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health. At present, China has not yet specified the substance of limited value, the present standards, the determination of these substances in cosmetics only provide detection methods. Cosmetics forty-one kinds of glucocorticoids Determination Liquid chromatography/tandem mass spectrometry and thin layer chromatography

1 Scope

This standard specifies the 41 kinds of cosmetics glucocorticoids liquid chromatography/tandem mass spectrometry and thin layer chromatography two kinds of measuring methods. This standard liquid chromatography/tandem mass spectrometry method used in cosmetics glucocorticoid quantitative determination, the detection limit 0.03μg/g, the limit of quantification was 0.1μg/g. The standard method is suitable for cosmetic tlc glucocorticoid qualitative screening. Spotting an amount of 10mg, the detection limit is 50μg/g; when spotting an amount of 20mg, the detection limit of 25μg/g.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1 -2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Glucocorticoid drugs belong to steroids, topical corticosteroids basic chemical structure as hydrocortisone structure. Namely containing Cyclopentane 17 carbon atoms, and hydrogen-phenanthrene nucleus, a methyl group on the C10 and C13 position, there are two carbon side chain at position C17, C3 and location keto C4-C5 Double bond. On this basis, the C1-C2 position dehydrogenation, C6-α methylate position, C9-α position fluoride, C11-hydroxyl modified and so form a Different types of applications, the strength of the different effects of glucocorticoid drugs. 4 liquid chromatography - tandem mass spectrometry 4.1 Principle Cosmetic Cream with saturated sodium chloride solution, dispersion, dispersed oil cosmetics with n-hexane, acetonitrile extract hormones from the dispersion Drugs, with zinc acetate and potassium ferrocyanide precipitate from the extract matrix macromolecules by solid phase extraction, purified by reverse phase high performance liquid chromatography / Tandem mass spectrometry, external standard. 4.2 Reagents and Standards 4.2.1 Methanol. chromatographically pure. 4.2.2 acetonitrile. chromatography. 4.2.3 acetic acid. Chromatographic pure. 4.2.4 n-hexane. AR.

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