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GB/T 23385-2009 English PDF

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GB/T 23385-2009: Determination of ampicillin in feed -- High performance liquid chromatography
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PDF similar to GB/T 23385-2009


Standard similar to GB/T 23385-2009

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Basic data

Standard ID GB/T 23385-2009 (GB/T23385-2009)
Description (Translated English) Determination of ampicillin in feed -- High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B46
Classification of International Standard 65.120
Word Count Estimation 6,648
Date of Issue 2009-03-26
Date of Implementation 2009-07-01
Regulation (derived from) National Standard Approval Announcement 2009 No.4 (Total No.144)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard provides high-performance liquid chromatography (HPLC) method for the determination of ampicillin feed. This standard applies to feed, concentrated feed and feed additives determination premixed ampicillin content. This standard detection limit of 1. 0 mg/kg, the limit of quantification of 5. 0 mg/kg.

GB/T 23385-2009: Determination of ampicillin in feed -- High performance liquid chromatography

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Determination of ampicillin in feed. High performance liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China Determination of ampicillin Feed High Performance Liquid Chromatography Posted 2009-03-26 2009-07-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points. This standard was drafted. Heilongjiang Province to monitor the feed, veterinary drugs. The main drafters of this standard. Jin Hui However, Ye Jianmin, Fan Xilong, Guo Wenxin, Tao Ya, Jiang Miao, Li Chunhui. Determination of ampicillin Feed High Performance Liquid Chromatography

1 Scope

This standard specifies a high performance liquid chromatography (HPLC) method for determination of feed ampicillin. This standard applies to feed, concentrated feed and feed additive Determination of Ampicillin Content premixed. The standard detection limit of 1.0mg/kg, the limit of quantification of 5.0mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT) GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT) Principle 3 Extracted with phosphate buffer sample ampicillin, HPLC reverse phase chromatography separation system, UV detector or diode Tube array detector at a wavelength of 220nm at the qualitative and quantitative determination.

4 Reagents and solutions

Unless otherwise specified in the analysis using only recognized as an analytical reagent and water in line with GB/T 6682 regulations. 4.1 acetonitrile. chromatography. 4.2 potassium dihydrogen phosphate, Ba (KH2PO4) = 0.02mol/L. Weigh accurately 2.72g of potassium dihydrogen phosphate in 1000mL volumetric flask, Dissolved in water, dilute to the mark. Extract 4.3. potassium dihydrogen phosphate solution (4.2) + acetonitrile (4.1) = 19 + 1. 4.4 HPLC mobile phase. 920mL take potassium dihydrogen phosphate solution (4.2) and 80mL acetonitrile (4.1), mixed by the 0.45μm Solvent microfiltration membrane filter, degassed prior to use ultrasound. 4.5 ampicillin stock standard solution. Weigh accurately ampicillin standard (content of not less than 86.5%) 50mg (accurate to 0.00001g), In 50mL volumetric flask with ultrapure water to dissolve and dilute to volume, shake. Its concentration of 1.0mg/mL, stored at 4 ℃ ~ 6 ℃ ice Box, valid 2d. 4.6 ampicillin standard working solution. accurate Pipette ampicillin stock standard solution (4.5) diluted with an appropriate amount of water to concentrations 1.0μg/mL, 5.0μg/mL, 10.0μg/mL, 25.0μg/mL, 50.0μg/mL, 100.0μg/mL, using now. 5. Apparatus 5.1 Analysis of balance. a sense of the amount of 0.0001g 0.00001g and one each in Taiwan. 5.2 High performance liquid chromatograph. with UV detector or diode array detector. 5.3 ultrasonic cleaning machine. 5.4 oscillator.

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