GB/T 23879-2009 PDF in English
GB/T 23879-2009 (GB/T23879-2009, GBT 23879-2009, GBT23879-2009)
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Feed additive -- Inositol
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Standards related to (historical): GB/T 23879-2009
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GB/T 23879-2009: PDF in English (GBT 23879-2009) GB/T 23879-2009
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.120
B 46
Feed additive - Inositol
饲料添加剂 肌醇
ISSUED ON: MAY 26, 2009
IMPLEMENTED ON: OCTOBER 1, 2009
Issued by: General Administration of Quality Supervision, Inspection and
Quarantine of PRC;
Standardization Administration of PRC.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Requirements ... 5
4 Test method ... 5
5 Inspection rules ... 10
6 Labeling, packaging, transportation, and storage ... 11
Appendix A (Informative) Inositol standard spectrum ... 12
Feed additive - Inositol
1 Scope
This standard specifies the technical requirements, test methods, inspection rules,
labeling, packaging, transportation, storage, etc. of the feed additive inositol.
This standard applies to inositol produced by hydrolysis of phytic acid or calcium
(magnesium) phytate, which is used as a nutritional feed additive in the feed industry.
Chemical Name: Inositol
Molecular formula:C6H12O6
Relative molecular mass: 180.16 (according to the 2007 International Relative Atomic
Mass)
Structural formula:
2 Normative references
The provisions in the following documents become the provisions of this standard
through reference in this standard. For the dated references, the subsequent amendments
(excluding corrections) or revisions do not apply to this standard, however, parties who
reach an agreement based on this standard are encouraged to study if the latest versions
of these documents are applicable. For undated references, the latest edition of the
referenced document applies to this standard.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
GB 10648 Feed label
GB/T 14699.1 Animal feeding stuffs - Sampling
2005 Edition of the Pharmacopoeia of the People’s Republic of China
4.2.1 Gravimetric method
4.2.1.1 Method principle
Inositol reacts with acetic anhydride under acidic conditions to form hexaacetyl inositol,
which is soluble in chloroform and insoluble in water, and the mass of hexaacetyl
inositol formed is then converted into the mass of inositol.
4.2.1.2 Reagents and solutions
4.2.1.2.1 Acetic anhydride dilute sulfuric acid solution: Add 1 mL of acetic anhydride
to 50 mL of 0.1 mol/L sulfuric acid solution.
4.2.1.2.2 Chloroform.
4.2.1.3 Instruments and equipment
4.2.1.3.1 General laboratory equipment.
4.2.1.3.2 Rotary evaporator.
4.2.1.4 Determination method
Weigh about 0.2 g of the sample (the weight shall be accurate to 0.0001 g) into a 250
mL beaker, add 5 mL of acetic anhydride dilute sulfuric acid solution (4.2.1.2.1), cover
it with a watch glass, and heat and dissolve in a water bath for 20 min; take it out to
cool, slowly add 100 mL of water, heat to boil for 20 min, and let it cool. Transfer it to
a separating funnel, wash the beaker with a little chloroform (4.2.1.2.2), pour the
washing liquid into the separating funnel; shake and extract 5 times with chloroform
(4.2.1.2.2), and the dosages of chloroform are 30 mL, 25 mL, 20 mL, 15 mL, and 10
mL, respectively. Wash the separatory funnel with 10 mL of chloroform, then wash
once with 10 mL of water, filter the chloroform layer with absorbent cotton, and wash
the water layer, filter and absorbent cotton with 10 mL of chloroform; combine the
filtrate and washing liquid in a pear-shaped flask with known weight (105°C), then
evaporate it on a rotary evaporator to remove the chloroform, and dry it at 105°C to
constant weight.
4.2.1.5 Calculation of results
Inositol content w1, in terms of mass fraction, is calculated according to the formula (1):
where:
m1 --- the mass sum of the pear-shaped flask and hexa-acetyl inositol after drying to
constant weight, in grams (g);
m2 --- The constant weight of the pear-shaped flask after drying, in grams (g);
m --- The mass of the sample, in grams (g);
0.4167 --- The coefficient of conversion from hexaacetyl inositol into inositol.
4.2.1.6 Repeatability
The difference between the absolute values of the two parallel determination results
shall be not more than 2.0%.
4.2.2 High-performance liquid chromatography (arbitration method)
4.2.2.1 Reagents and solutions
4.2.2.1.1 Double-distilled water: It shall meet the first-grade water specified in GB/T
6682.
4.2.2.1.2 Standard solution: Weigh 1.0 g (the weight shall be accurate to 0.0001 g) of
inositol dry reference substance (the purity shall be ≥98.5%) into a 100 mL volumetric
flask, add an appropriate amount of distilled water, dissolve it with ultrasonic waves,
and then use distilled water to dilute it to the mark and shake well.
4.2.2.2 Instruments and equipment
4.2.2.2.1 General laboratory equipment.
4.2.2.2.2 Ultrasonic cleaner.
4.2.2.2.3 High-performance liquid chromatograph.
4.2.2.3 Preparation of sample solution
Weigh about 1.0 g of the sample (the weight shall be accurate to 0.0001 g) into a 100
mL volumetric flask, add an appropriate amount of distilled water, dissolve it by
ultrasonication, then dilute to the mark with distilled water, and shake well for later
testing.
4.2.2.4 Determination
4.2.2.4.1 Chromatographic conditions
Chromatographic column: a strong cation exchange column (calcium ion type).
4.5 Determination of heavy metals (by Pb)
The determination shall be carried out according to the regulations of “Heavy metal
inspection method - Method I”, Appendix VIII H of Part 2 of the 2005 edition of
“Pharmacopoeia of the People’s Republic of China”.
4.6 Determination of arsenic (As)
The determination shall be carried out according to the regulations of “Arsenic salt
inspection method”, Appendix VIII J of Part 2 of the 2005 edition of “Pharmacopoeia
of the People’s Republic of China”.
4.7 Determination of melting point
The determination shall be carried out according to the regulations of “Melting point
determination method”, Appendix VI C of Part 2 of the 2005 edition of “Pharmacopoeia
of the People’s Republic of China”.
5 Inspection rules
5.1 Sampling method
Sampling shall be carried out according to GB/T 14699.1.
5.2 Factory inspection
5.2.1 Batch formation
Products with the same raw material, same formula, and same shift are regarded as a
batch, and each batch of products is subject to factory inspection.
5.2.2 Factory inspection items
Sensory characteristics, loss on drying, inositol content, melting point.
5.2.3 Judgment method
Based on the relevant test methods and requirements of this standard, the selected
samples shall be inspected according to the factory inspection items. If one of the
indicators of the inspection results does not meet the requirements of this standard, re-
inspection shall be carried out by sampling from double packaging units. If there is still
any one of the re-inspection results that does not meet the requirements of the standard,
the batch of products shall be judged as unqualified and cannot leave the factory.
5.3 Type inspection
5.3.1 In one of the following situations, type inspection shall be carried out:
a) The formula or production process is changed;
b) During every six months of normal production; or, when the production resumes
after six months of suspension;
c) When the national technical supervision department requests an inspection.
5.3.2 Type inspection includes all items in Chapter 3 of this standard.
5.3.3 Judgment method: Based on the relevant test methods and requirements of this
standard, the selected samples shall be inspected according to the type inspection items.
If one of the indicators of the inspection results does not meet the requirements of this
standard, re-inspection shall be carried out by sampling from double packaging units.
If there is still any one of the re-inspection results that does not meet the requirements
of this standard, the type inspection shall be judged as unqualified.
6 Labeling, packaging, transportation, and storage
6.1 Labeling
The labeling shall be carried out according to GB 10648.
6.2 Packaging
The inner packaging of this product shall be made of food-grade polyethylene film, and
the products shall be sealed in an appropriate container. The packaging shall meet the
requirements of transportation and storage. The quality of each packaging shall be able
to be determined according to customer requirements.
6.3 Transportation
Exposure to sun, rain, heat, and impact shall be avoided. It shall be handled with care
when loading and unloading and not be mixed with toxic, harmful, or other polluting
items during packing and transportation.
6.4 Storage
This product shall be stored in a ventilated, dry, and pollution-free place.
Under the specified storage conditions, the product has a shelf life of 48 months in the
original package (after being opened, it shall be used as soon as possible to avoid
deterioration).
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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