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GB/T 21330-2007: Method for the determination of streptomycin residues in animal original food -- Enzyme-linked immunosorbent assay
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21330-2007 | English | 209 |
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Method for the determination of streptomycin residues in animal original food -- Enzyme-linked immunosorbent assay
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GB/T 21330-2007
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Basic data | Standard ID | GB/T 21330-2007 (GB/T21330-2007) | | Description (Translated English) | Method for the determination of streptomycin residues in animal original food -- Enzyme-linked immunosorbent assay | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 67.050 | | Word Count Estimation | 8,817 | | Date of Issue | 2007-10-31 | | Date of Implementation | 2008-04-01 | | Quoted Standard | GB/T 6379 | | Regulation (derived from) | China National Standard Approval Announcement2007 No.13 (Total No.113) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the meat, enzyme-linked immunoassay streptomycin residues in milk and milk powder volume method, viscera, aquatic products. This standard specifies the meat, offal, fish, enzyme-linked immunoassay milk and milk powder streptomycin residues. This standard method detection limit: meat and aquatic products as 50. 0ug/kg, viscera, milk and milk powder as 20. 0ug/kg. | GB/T 21330-2007: Method for the determination of streptomycin residues in animal original food -- Enzyme-linked immunosorbent assay
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Method for the determination of streptomycin residues in amminal original food. Enzyme-linked immunosorbent assay
ICS 67.050
C53
National Standards of People's Republic of China
Foodstuffs of animal origin determination of streptomycin residues in
ELISA
Posted 2007-10-31
2008-04-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The Standard Appendix A, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Shi Weiliang, Zhang Xiaofeng, Zhu Zhenjiang, Lihao Yan, Wu Shan.
This standard is a national standard was first published.
Foodstuffs of animal origin determination of streptomycin residues in
ELISA
1 Scope
This standard specifies the method of enzyme-linked immunosorbent assay of streptomycin residues in meat, offal, fish, milk and milk powder.
This standard applies to meat, offal, fish, milk and milk powder Determination of streptomycin residues.
The standard method detection limit. meat, offal and fish as 50.0μg/kg; milk and milk powder as 20.0μg/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently
Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6379 (all parts) of measurement methods and results Accuracy (trueness and precision)
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
Principle 3
Microplate coated with sheep anti-rabbit IgG antibody was added specific antibody (anti-rabbit antibody streptomycin), enzyme-labeled streptomycin, streptomycin standard
After registration or sample extract specific antibody coated sheep anti-rabbit IgG antibody, while the free enzyme-labeled streptomycin and streptomycin
And specific antibody binding competitive. Remove unbound enzyme-labeled streptomycin and streptomycin by washing, followed by addition of chromogenic substrate, enzyme
Standard absorbance was measured according to the content of the sample absorbance worth streptomycin.
4 Reagents and materials
Remove otherwise stated, all reagents were of analytical grade water as a water GB/T 6682 regulations.
4.1 streptomycin detection kit (composition see Appendix A).
4.2 TCA.
4.3 sodium chloride.
4.4 potassium chloride.
4.5 potassium dihydrogen phosphate.
4.6 dibasic sodium phosphate.
4.7 sodium dihydrogen phosphate.
4.8 Tween-80.
4.9 3% TCA. weigh 3g TCA (4.2), water volume to 100mL.
4.10 PBS buffer. 8.5g sodium chloride, 1.15g disodium hydrogen phosphate, 0.27g sodium dihydrogen phosphate, water volume to 1L.
4.11 SDB buffer. 1.15g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, 0.2g potassium chloride, 30g sodium chloride, 0.5mL spit
-80 Temperature, water volume to 1L.
4.12 hexane.
4.13 streptomycin standard substance. purity greater than or equal to 98%.
4.14 streptomycin preparation of standard solution. sterile water as the solvent formulated as 25mg/mL stock solution, at -20 ℃ conditions
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