GB/T 22246-2025 PDF English
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| GB/T 22246-2025 | English | 215 |
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Determination of pantothenic acid in health foods
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| GB/T 22246-2008 | English | 90 |
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Determination of calcium pantothenate in health foods
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GB/T 22246-2025: Determination of pantothenic acid in health foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT22246-2025
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 67.050
CCS X 83
Replacing GB/T 22246-2008
Determination of Pantothenic Acid in Health Foods
Issued on: JANUARY 24, 2025
Implemented on: AUGUST 1, 2025
Issued by. State Administration for Market Regulation;
Standardization Administration of the People’s Republic of China.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative References... 4
3 Terms and Definitions... 4
4 Principle... 4
5 Reagents or Materials... 4
6 Instruments and Equipment... 6
7 Analytical Procedures... 6
8 Calculation and Expression of Results... 8
9 Precision... 9
10 Detection Limit and Quantification Limit... 9
Appendix A (informative) High-performance Liquid Chromatograms of Pantothenic
Acid Standard Solution and Specimen... 10
Determination of Pantothenic Acid in Health Foods
1 Scope
This document describes a high-performance liquid chromatography (HPLC) for the
determination of pantothenic acid in health foods.
This document is applicable to the determination of pantothenic acid in health foods in dosage
forms, such as tablets, hard capsules, soft capsules, granules, powders, oral liquids and jelly
candies, etc.
2 Normative References
The contents of the following documents constitute indispensable clauses of this document
through the normative references in the text. In terms of references with a specified date, only
versions with a specified date are applicable to this document. In terms of references without a
specified date, the latest version (including all the modifications) is applicable to this document.
GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods
3 Terms and Definitions
This document does not have terms or definitions that need to be defined.
4 Principle
Pantothenic acid in the specimen is extracted with hot water, separated by high-performance
liquid chromatography, and detected by ultraviolet light. Qualitative determination is based on
retention time, and quantitative determination is based on the external standard method.
5 Reagents or Materials
Unless otherwise specified, only analytically pure reagents shall be used.
5.1 Reagents
5.1.1 Water, Grade 1 water as specified in GB/T 6682.
5.1.2 Hydrochloric acid (HCl).
5.1.3 Sodium hydroxide (NaOH).
5.1.4 Phosphoric acid (H3PO4).
100 mL volumetric flasks, add water to dilute to the scale and mix them well to obtain
pantothenic acid series standard working solutions with concentrations of 1 g/mL, 2 g/mL,
4 g/mL, 8 g/mL, 16 g/mL and 32 g/mL. Prepare immediately prior to use.
5.4 Materials
Filter membrane. 0.45 m, aqueous.
6 Instruments and Equipment
6.1 High-performance liquid chromatograph. equipped with UV detector or equivalent.
6.2 Balance. with a division value of 0.001 g and 0.1 mg.
6.3 Constant-temperature oscillating water bath. with an oscillating frequency of 100 r/min
20 r/min.
6.4 Ultrasonic cleaner.
6.5 pH meter. with an accuracy of 0.01.
6.6 High-speed centrifuge. speed not less than 8,000 r/min.
7 Analytical Procedures
7.1 Specimen Preparation
7.1.1 Solid specimens (hard capsules, tablets and powders, etc.). take at least 20 capsules or at
least 5 g of sample (for hard capsules, remove the shells and take the contents; for tablets, crush
them), finely grind (if necessary), mix well and set aside.
7.1.2 Solid specimens (jelly candies, etc.). take at least 10 capsules (for mixed specimens of
different colors or flavors, evenly conduct the sampling by color or flavor) or at least 10 g of
sample, cut into small pieces and set aside.
7.1.3 Semi-solid specimens (soft capsules, etc.). take at least 20 capsules or at least 5 g of
sample, remove the shells, take the contents, mix well and set aside.
7.1.4 Liquid specimens (oral liquids, etc.). take 5 minimum-size packages or at least 50 mL of
sample (if the sample contains carbonic acid, use ultrasound to remove carbon dioxide), mix
well and set aside.
7.2 Specimen Preparation
Weigh-take 1 g ~ 3 g of specimen (accurate to 0.001 g), or transfer-take 1 mL ~ 3 mL of
specimen, or a volume that obtains approximately 1 mg of pantothenic acid in the specimen.
Place it in a 100 mL conical flask. Add 30 mL of warm water at 40 °C ~ 50 °C; vortex or stir it,
until the sample is thoroughly dissolved. Perform ultrasound for 20 minutes, and cool to room
temperature. Use 0.1 mol/L hydrochloric acid solution (5.1.8), 1.0 mol/L hydrochloric acid
solution (5.1.9), 0.01 mol/L sodium hydroxide solution (5.1.10) or 0.1 mol/L sodium hydroxide
solution (5.1.11) to adjust the pH to 5.0.Add 5 mL of 0.5 mol/L zinc sulfate solution (5.1.12)
and mix it thoroughly. Transfer to a 50 mL volumetric flask, use water to dilute to the scale,
mix it thoroughly, and transfer to a centrifuge tube. At 8,000 r/min, centrifuge for 2 min. Pass
the supernatant through a 0.45 m filter membrane (5.4) and prepare the filtrate for
determination on the machine.
NOTE. if necessary, based on the content of the components in the specimen, appropriately increase
the dilution factor f to ensure that the mass concentration of pantothenic acid in the
specimen is within the range of 1.0 g/mL ~ 32.0 g/mL.
7.3 Chromatographic Reference Conditions
The chromatographic reference conditions are as follows.
a) Chromatographic column. C18 (particle size 5 m, 250 mm 4.6 mm), or equivalent
chromatographic column;
b) Mobile phase. potassium dihydrogen phosphate solutionacetonitrile (5.1.14);
c) Flow rate. 1 mL/min;
d) Column temperature. 35 °C;
e) Injection volume. 10 L or 20 L;
f) Detection wavelength. 200 nm.
7.4 Standard Curve Determination
Successively inject the pantothenic acid standard working solution into the high-performance
liquid chromatograph and determine the peak area of each component. With the mass
concentration of the corresponding standard working solution as the x-coordinate and the peak
area as the y-coordinate to draw a standard curve. The high-performance liquid chromatogram
of the pantothenic acid standard solution (16 g/mL) is shown in Figure A.1 in Appendix A.
7.5 Determination of Specimen Solution
Inject the specimen solution into the high-performance liquid chromatograph to obtain the
corresponding peak area. In accordance with the standard curve, use the external standard
method to calculate the mass concentration of pantothenic acid in the specimen solution to be
determined. The high-performance liquid chromatogram of pantothenic acid in the specimen
(tablets) is shown in Figure A.2.
Perform two tests in parallel.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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