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GB 25535-2010: National food safety standards of food additives gellan gum
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National food safety standards of food additives gellan gum
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GB 25535-2010
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Basic data | Standard ID | GB 25535-2010 (GB25535-2010) | | Description (Translated English) | National food safety standards of food additives gellan gum | | Sector / Industry | National Standard | | Classification of Chinese Standard | X40 | | Classification of International Standard | 67.220.20 | | Word Count Estimation | 9,966 | | Date of Issue | 2010-12-21 | | Date of Implementation | 2011-02-21 | | Regulation (derived from) | Ministry of Health Bulletin No. 19 of 2010 | | Issuing agency(ies) | Ministry of Health of the People's Republic of China | | Summary | This Chinese standard applies to Elodea aeruginosa (Pseudomonas elodea) for pure culture fermentation of carbohydrates, processed food additives made ??of gellan gum. | GB 25535-2010: National food safety standards of food additives gellan gum---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National food safety standards of food additives gellan gum
National Food Safety Standard
Food Additives gellan gum
Issued on. 2010-12-21
2011-02-21 implementation
National Standards of People's Republic of China
People's Republic of China Ministry of Health issued
Foreword
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
National Food Safety Standard
Food Additives gellan gum
1 Scope
This standard applies to the Elodea aeruginosa (Pseudomonas elodea) of carbohydrates pure culture fermentation, by adding
Food Additives workers made gellan gum.
2 Normative references
The standard file referenced in the application of this standard is essential. For cited documents with dates, only the date of
Version applies to this standard. For undated references, the latest edition (including any amendments) applies to this standard.
3 molecular structure and molecular weight
3.1 Molecular Structure
The basic unit consists of one molecule of glucuronic acid molecule rhamnose and two molecules of glucose repeating polymeric composition.
3.2 relative molecular mass
4 × 105 ~ 6 × 105 (according to 2007 international relative atomic mass)
4. Technical Requirements
4.1 Sensory requirements. comply with Table 1.
Table 1 Sensory requirements
Project requires test methods
Color white take appropriate sample is placed in a clean, dry white porcelain dish, in natural light
Under observe its color and texture. Organization Status powder
4.2 Physical indicators. to comply with Table 2.
Table 2. Physical and chemical indicators
4.3 microbial indicators. to comply with Table 3.
Item Index Test Method
Gellan gum, w /% 85.0 ~ 108.0 Appendix A A.3
Loss on drying, w /% ≤ 15.0 GB 5009.3-2010 a direct drying
Lead (Pb)/(mg/kg) ≤ 2 GB 5009.12
Isopropanol b/(mg/kg) ≤ 750 Appendix B
a drying temperature and time are 105 ℃ and 2.5h.
b limited to non-ethanol processing gellan gum products.
Table 3 microbiological indicators
Item Index Test Method
Cfu/(CFU/g) ≤ 10000 GB 4789.2
Coliform/(MPN/100g) ≤ 30 GB 4789.3
Salmonella 0/25g GB 4789.4
Molds and yeasts/(CFU/g) ≤ 400 GB 4789.15
Appendix A
(Normative)
Testing method
A.1 General Provisions
Unless otherwise indicated in the analysis using only recognized as analytical reagents and GB/T 6682-2008 specified in the water. In the analysis
Standard titration solution, impurities measured by standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601, GB/T
602, the provisions of preparation GB/T 603's. This solution was used in the test does not indicate what is formulated with solvent, it refers to an aqueous solution.
A.2 Identification Test
A.2.1 Solubility Test
Dissolved in water, forming a viscous solution; insoluble in ethanol.
A.2.2 calcium gel test
1g samples were dissolved in 99mL water, formulated as a 1% solution. With a mechanical stirrer and a propeller-shaped stirring blades, stirring the
Solution 2h (left portion of the solution when used in A.2.3), with a wide bore pipette to take a small amount of the solution, which was moved CaCl2 solution (100g/L), and made
I.e. form a gel.
A.2.3 sodium gel test
A.2.2 formulated to 1% of the sample was added 0.5g NaCl, and the solution is heated to 80 ℃, with constant stirring and at 80 ℃ condition
1min, to stop heating and stirring, cooled to room temperature to form a hard gel.
A.3 Determination of cold glue knot
A.3.1 Reagents and materials
a) diatomaceous earth. chromatography.
b) ethanol.
c) ethanol. 7822.
A.3.2 Instruments and Equipment
a) glass filter.
b) a dryer (180 mm).
A.3.3 Analysis step
Weigh about 1.0g HPLC grade diatomaceous earth, placed in a glass filter, uniform paving, 105 ℃ dried 5h, in a desiccator
Accurately weighed after cooling. It weighs about 0.2g after drying (105 ℃, 2.5h) sample (accurate to 0.001g), add 50mL of water at about 80 ℃
Water bath and stirred to dissolve 30min. Added 200mL pre-heated to 60 ℃ ~ 70 ℃ absolute ethanol, mixed, allowed to stand for 12h, spend
Said glass containing celite filter, and the filter residue was washed 5 times with ethanol, washed 3 times before each dosage 20mL, twice with
The amount of each wash 10 mL. The residue at 105 ℃ drying 5h, accurately weighed after cooling in a desiccator.
A.3.4 Calculation Results
Gellan gum content X1 according to formula (A.1) Calculated.
1 × = m
mX% (A.1)
Where.
X1-- gellan gum content,%;
m1-- residue mass in grams (g);
m0-- sample mass, in grams (g).
The results parallel arithmetic mean of the measurement results shall prevail. Twice in the same condition absolutely independent determination results
The difference is not more than 2% of the arithmetic mean.
Appendix B
(Informative)
Determination of isopropanol
B.1 Reagents and materials
a) isopropanol. chromatography.
b) tert-butanol. HPLC grade.
B.2 instruments and equipment
a) Gas chromatograph. with hydrogen flame ionization detector.
b) Column. 1.8m × 2.3mm (id) stainless steel column or an equivalent efficacy column filled 0.2mm ~ 0.15 mm of Porapak QS
Fillers or other equivalents.
B.3 reference chromatographic conditions
a) carrier gas. helium.
b) flow rate. 80 mL/min.
c) Inlet temperature.200 ℃.
e) Column temperature. 165 ℃.
f) Detector temperature.200 ℃.
g) Injection volume. 5μL.
h) isopropanol retention time of approximately 2min, t-butanol retention time of approximately 3min.
B.4 analysis step
B.4.1 Preparation of standard solutions
B.4.1.1 isopropanol (IPA) standard solution
Accurately weigh 500.0 mg of isopropanol, transferred to a 50mL volumetric flask, and diluted with water to volume, and mix. Draw 10mL
The solution was transferred to a 100mL volumetric flask, and diluted with water to volume, and mix.
B.4.1.2 t-butanol (TBA) standard solution
Accurately weigh 500.0 mg of tert-butanol and transferred to a 50mL volumetric flask, and diluted with water to volume, and mix. Draw 10mL
The solution was transferred to a 100mL volumetric flask, and diluted with water to volume, and mix.
B.4.1.3 mixed standard solution
Pipette 4mL isopropyl alcohol (IPA) and tert-butyl alcohol (TBA) standard solution was transferred to a 125 mL Erlenmeyer flask, add water to dilute
Diluted to about 100mL, and mix. Each ml of this solution contains about 40 μg and 40 μg of isopropanol tert-butanol.
B.4.2 samples were prepared
In 200 mL of water containing 1000 mL round-bottomed distillation flask, 1mL Suitable defoamers (such as Dow-Corning G-10 or equivalent
Thereof), and dispersed. Join accurately weighed sample of about 5g gellan gum, shaking 1h. The bottle was connected to a distillation column, adjust the temperature,
So that the foam can not enter the column, then the distillate of about 100mL. Added 4.0mL of tert-butanol in the distillate (TBA) standard solution to prepare test
Sample solution.
B.4.3 Determination
5μL injection mixed standard solution was measured at reference chromatographic conditions, record isopropanol peak areas AIPA, tert-butyl alcohol
Peak areas ATBA, calculate the relative correction factor f (f = AIPA/ATBA).
Similarly, injection 5μL sample solution, under reference chromatographic conditions were measured peak area values recorded isopropanol aIPA, tert-butanol
The peak areas aTBA.
B.5 Calculation Results
Isopropanol content X according to the formula (B.1) Calculated.
maf
aX
TBA
IPA
××
× = 4000 (B.1)
Where.
X-- isopropyl alcohol content in milligrams per kilogram (mg/kg);
aIPA-- sample liquid chromatographic isopropanol mean peak area;
f-- relative correction factor (f = AIPA/ATBA);
aTBA-- sample liquid t-butanol mean peak area;
m-- saying that the quality of the sample taken, in grams (g).
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