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SB/T 10386-2004: Determination of chloramphenicol in meat of livestock and poultry
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Determination of chloramphenicol in meat of livestock and poultry
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SB/T 10386-2004
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Basic data | Standard ID | SB/T 10386-2004 (SB/T10386-2004) | | Description (Translated English) | Determination of chloramphenicol in meat of livestock and poultry | | Sector / Industry | Domestic Trade Industry Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.120 | | Word Count Estimation | 5,547 | | Date of Issue | 2004-08-04 | | Date of Implementation | 2004-09-01 | | Issuing agency(ies) | People Republic of China Ministry of Commerce | | Summary | This standard specifies: meat, poultry, chloramphenicol Determination, This standard applies to: meat, poultry, chloramphenicol test. | SB/T 10386-2004: Determination of chloramphenicol in meat of livestock and poultry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chloramphenicol in meat of livestock and poultry
ICS 67.120
X04
Record number. 14167-2004
People's Republic of China domestic trade industry standard
Determination of chloramphenicol in livestock and poultry meat
Released on.2004-08-04
2004-09-01 implementation
Published by the Ministry of Commerce
Foreword
This standard was proposed by the Ministry of Commerce of the People's Republic
This standard was drafted by the Department of Commerce's Slaughter Technology Appraisal Center. The Center for Nutrition and Food Safety of the Chinese Center for Disease Control and Prevention, Hebei Province
The Center for Disease Control and Prevention participated in the drafting.
The main drafters of this standard. Liu Wenjuan, Yang Zuying, Song Shufeng, Han Huixin, Zhang Xinling, Liu Hucheng, Wang Guiji.
This standard is entrusted to the Ministry of Commerce's Slaughter Technology Appraisal Center for interpretation.
Determination of chloramphenicol in livestock and poultry meat
1 Scope
This standard specifies the method for determination of chloramphenicol in livestock and poultry.
This standard applies to the detection of chloramphenicol in livestock and poultry.
2 Principle
The residual chloramphenicol in the sample was extracted with ethyl acetate, concentrated under reduced pressure, and the residue was dissolved with 0.5 mol/L perchloric acid, and the mixture was treated with n-hexane.
In addition to fat, after filtration through a microporous membrane, the filtrate was analyzed by high performance liquid chromatography.
3 reagents and materials
The reagents used below are analytically pure reagents unless otherwise specified, and the water is re-distilled water.
3.1 Methanol. chromatographically pure.
3.2 n-hexane.
3.3 Chloramphenicol is equivalent to 994 units per milligram, content ≥ 99%.
3.4 Ethyl acetate.
3.5 Perchloric acid.
3.6 Chloramphenicol standard stock solution. accurately weigh 0.010g chloramphenicol standard, put it in a 100mL volumetric flask, add methanol to dissolve and dilute
To the scale, shake well. The concentration of this solution was 0.10 mg/mL, which was stored in a refrigerator and was valid for 3 months.
3.7 Chloramphenicol standard working solution. Accurately absorb 5.0mL of stock solution in a 50mL volumetric flask and dilute to the mark with methanol. This solution is thick
The temperature was 10 μg/mL and stored in the refrigerator. Valid for half a month. Before use, take this liquid and dilute it with 0.5 mol/L perchloric acid to the appropriate concentration.
Quasi-working fluid.
4 Instruments and equipment
4.1 High Performance Liquid Chromatograph (with UV detector).
4.2 Rotary evaporator.
4.3 Eddy current mixer.
4.4 Ultrasonic generator.
4.5 Rotate the evaporator.
4.6 Microfiltration membrane filtration.
5 Determination steps
5.1 extraction
Take.200g of sample and grind it, weigh 20g (accurate to 0.01g) of the ground sample, place it in a 100mL stoppered flask, and add
40 mL of ethyl acetate, shaken, sonicated for 30 min, filtered, and the filtrate was placed in another flask. Add 20mL of ethyl acetate to try again
In the sample, ultrasonic extraction for 15 min, filtration, the filtrate was combined, and the filter residue was further extracted with 20 mL of ethyl acetate for 15 min, filtered, and the filtrate was combined.
The filtrate was combined and transferred to a concentrate bottle.
5.2 Purification
The combined filtrate was concentrated to dryness on a rotary bath of 52 ° C to 55 ° C. Accurately add 1.0mL0.5mol/L perchloric acid solution
The residue in the concentrate bottle was washed, 1 mL of n-hexane was added, shaken for 1 min, and the whole solution was transferred to a 5 mL test tube, and the layer was static.
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