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GB 31624-2014: National Food Safety Standard -- Food Additive -- Natural carotene
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| GB 31624-2014 | English | 189 |
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National Food Safety Standard -- Food Additive -- Natural carotene
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GB 31624-2014
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Basic data | Standard ID | GB 31624-2014 (GB31624-2014) | | Description (Translated English) | National Food Safety Standard -- Food Additive -- Natural carotene | | Sector / Industry | National Standard | | Classification of Chinese Standard | C53 | | Classification of International Standard | 67.020 | | Word Count Estimation | 8,820 | | Date of Issue | 1/28/2015 | | Date of Implementation | 7/28/2015 | | Regulation (derived from) | National Health and Family Planning Committee Announcement 2015 No. 2 | | Issuing agency(ies) | National Health and Family Planning Commission of the People's Republic of China | | Summary | This Standard applies to carrots (Daucus carota), oil palm fruit (Elaeis guinensis), sweet potatoes (Ipomoea batatas) or other edible plants as raw material, solvent extraction, refining of food additives natural carotene. The main coloring substance ��- c | GB 31624-2014: National Food Safety Standard -- Food Additive -- Natural carotene---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(National food safety standards for food additives natural carotene)
National Standards of People's Republic of China
National Food Safety Standard
Natural carotene food additives
Published 2015-01-28
2015-07-28 implementation
People's Republic of China
National Health and Family Planning Commission issued
National Food Safety Standard
Natural carotene food additives
1 Scope
This standard applies to carrot (Daucuscarota), palm fruit oil (Elaeisguinensis), sweet potatoes (Ipomoeabatatas) or
Other edible plants as raw material, solvent extraction, refining natural carotene food additives. The main coloring substances β- carrot
Su and α- carotene, β- carotene majority.
Molecular formula, structural formula and relative molecular mass of the main component 2
Formula 2.1
C40H56 (β- carotene)
2.2 Structure
2.3 Molecular Weight
536.88 (β- carotene, according to international relative atomic mass in 2007)
3 Technical requirements
3.1 Sensory requirements
Shall comply with the requirements in Table 1.
Table 1 Sensory requirements
Project requires test methods
Brown or red-brown color to orange to dark orange
Solid or liquid state
The sample is placed in the appropriate uniform white porcelain plate, its color was observed under natural light.
And state
3.2 Physical and Chemical Indicators
Shall be in accordance with Table 2.
Table 2. Physical and chemical indicators
Item Index Test Method
Carotene content (in terms β- carotene, w) /% conform claimed in Appendix A A.4
Residual solvent (acetone, hexane, methanol, ethanol and isopropanol)/(mg/kg) ≤ 50 (alone or in combination) A.4
Lead (Pb)/(mg/kg) ≤ 5 GB 5009.12
NOTE. commercial product should be natural carotene natural carotene compliance with this standard as a raw material, may be added antioxidants, emulsifiers and the like materials, which
Formulated as a suspension in an aqueous suspension or oil-type powder.
Appendix A
Testing method
A.1 General Provisions
Unless otherwise specified in this standard, the reagents were of analytical grade, standard titration solution used, the impurity determined by standard solution, and made preparations
Product, should GB/T 601, GB/T 602, GB/T 603 provisions prepared test water shall meet the GB/T 6682 in a predetermined three water.
The test does not indicate when the solution which is formulated with a solvent, refer to the aqueous solution.
A.2 Identification Test
A.2.1 solubility test
Insoluble in water.
A.2.2 Spectrophotometers
Measured using a spectrophotometer, a cyclohexane solution of the sample (5mg/L) in the most 440nm ~ 457nm 470nm ~ 486nm and at
Large absorption values.
A.2.3 color reaction
A sample of a toluene solution (containing about β- carotene 400μg/mL) on the filter paper points, antimony into a toluene solution with 200g/L trichloride
Spray line, after 2min ~ 3min, blue spots.
A.3 Determination of carotene content
A.3.1 Reagents and materials
A.3.1.1 chloroform.
A.3.1.2 cyclohexane.
A.3.2 Instruments and Equipment
Spectrophotometer.
A.3.3 Analysis step
Preparation of sample solution A.3.3.1
Sample Weigh 0.08g ± 0.01g, placed in a 100mL volumetric flask, add 20mL sample dissolution chloroform, diluted with cyclohexane
To the mark. Take 5.0 mL of this sample solution, diluted further with cyclohexane 100mL flask to the mark. Take this dilution
5.0 mL solution, diluted in a third 100mL flask with cyclohexane, shaken to give a final sample solution.
A.3.3.2 Determination
The sample solution was placed in a 1cm cuvette with cyclohexane as a blank using a spectrophotometer at wavelength of maximum absorption (440nm ~
) Measuring the absorbance 457nm. Absorbance should be controlled between 0.2 and 0.8, or the amount of sample should be adjusted by adjusting the concentration of the sample solution, said weight and then
The new absorbance was measured.
Note. The above operation should be completed as soon as possible to avoid exposure to air, should ensure that all operations are to avoid direct sunlight.
A.3.4 calculation results
Carotene content (in terms β- carotene) by mass fraction w1 of formula (A.1) is calculated.
w1 =
A × 40000
m1 × 100
2500 × 100%
(A.1)
Where.
A --- the diluted sample absorbance of the solution;
--- 40000 dilution;
M1 --- mass of sample, grams (G);
100 --- conversion factor;
2500 --- β- carotene absorption coefficient in cyclohexane.
The test result to the arithmetic mean of replicates results.
A.4 Determination of residual solvent (acetone, hexane, methanol, ethanol and isopropanol) of
A.4.1 Reagents and materials
A.4.1.1 Component Reference Sample tested. acetone, hexane, methanol, ethanol and isopropanol.
A.4.1.2 blank. almost no sample solvent.
A.4.1.3 3- methyl-2-pentanone.
A.4.1.4 methanol.
A.4.1.5 water. a water GB/T 6682-2008 specified.
A.4.2 Instruments and Equipment
Gas chromatograph with flame ionization detector (FID) and a headspace sampler.
A.4.3 Reference chromatographic conditions
A.4.3.1 Column. fused Shi Ying, 0.8m long, 0.53mm inner diameter, the coating is 100% dimethyl polysiloxane, a coating thickness of 1μm.
Supporting. fused Shi Ying, length 30m, internal diameter 0.53mm, a coating of 10% dimethyl polysiloxane coating thickness of 5μm. Or other equivalent
Separation column and the chromatographic conditions.
A.4.3.2 carrier gas. helium.
A.4.3.3 flow rate. 208kPa, 5mL/min.
A.4.3.4 Column temperature. 35 ℃ maintained 5min, at 5 ℃/min temperature was raised to 90 ℃, maintained 6min.
A.4.3.5 Inlet temperature. 140 ℃.
A.4.3.6 Detector temperature. 300 ℃.
A.4.4 headspace sampler reference condition
A.4.4.1 Sample heating temperature. 60 ℃.
A.4.4.2 sample heating time. 10min.
A.4.4.3 Injector temperature. 70 ℃.
A.4.4.4 mass transfer temperature. 80 ℃.
A.4.4.5 sample gas injection. splitless mode, 1.0mL.
A.4.5 Analysis step
Preparation of internal standard solution A.4.5.1
50.0mL of methanol pipetted into a 50mL sample vial, capped vials weighed to the nearest 0.0001g. Pipette 15μL of internal standard
Was 3-methyl-2-pentanone, which is injected through the septum into the sample vial, mixed, and weighed vials, accurate to 0.0001g.
Preparation of blank solution A.4.5.2
Weigh 0.20g blank, placed in an injection vial. Was added 5.0mL of methanol and 1.0mL internal standard solution. For 10min at 60 ℃,
Shake vigorously 10s, and mix.
Preparation of sample solution A.4.5.3
0.20g sample was weighed, placed into vials. Was added 5.0mL of methanol and 1.0mL internal standard solution. For 10min at 60 ℃,
Shake vigorously 10s, and mix.
Preparation of calibration solution A.4.5.4
Solution A. 50.0mL of methanol pipetted into a 50mL sample vial, capped vials weighed to the nearest 0.0001g. Pipette 50μL
Reference component to be measured, which is injected through the septum into the sample vial, mixed, and weighed vials, accurate to 0.0001g.
Weigh 0.20g blank, placed in an injection vial. Was added 4.9mL of methanol and 1.0mL internal standard solution. 0.1mL solution injected through the septum
Liquid A. Uniformly mixed for 10min at 60 ℃, shake vigorously 10s.
A.4.6 Determination
A.4.3 and A.4.4 in the reference operation conditions, respectively, of the sample solution, calibration solution and a blank solution chromatographed.
A.4.7 calculation results
A.4.7.1 calibration factor fi
Fi calibration factor according to equation (A.2) is calculated.
fi =
mi
m0 × (Af-Ag) × 10
(A.2)
Where.
--- mass mi The solution A and the reference component to be measured in milligrams (mg);
--- the mass of the internal standard solution M0 standard material, in milligrams (mg);
Peak area ratio of component to be measured and the internal standard peak area of the AF calibration solution chromatogram ---;
Peak area ratio of component to be measured and the internal standard peak area in blank solution AG --- chromatogram;
10 --- concentration conversion coefficient.
A.4.7.2 component to be tested
Content of the component to be measured (acetone, hexane, methanol, ethanol and isopropanol) wi of milligrams per kilogram (mg/kg) basis, according to equation (A.3)
Calculation.
mi =
Ai × m0 × fi × 1000
50 × m2
(A.3)
Where.
Peak area ratio of component to be measured and the internal standard peak area of sample solution --- AI in the chromatogram;
--- the mass of the internal standard solution M0 standard material, in milligrams (mg);
--- Fi calibration factor;
1000 --- mass conversion factor;
50 --- volume conversion factor;
--- M2 sample mass, in grams (g).
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