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SN/T 4624.9-2016 English PDF

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SN/T 4624.9-2016: Methods for examination of import microbial blends in the environmental protection—Part 9: Diarrheogenic Escherichia coli
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SN/T 4624.9-2016English419 Add to Cart 4 days [Need to translate] Methods for examination of import microbial blends in the environmental protection—Part 9: Diarrheogenic Escherichia coli Valid SN/T 4624.9-2016

PDF similar to SN/T 4624.9-2016


Standard similar to SN/T 4624.9-2016

HG/T 5871   GB/T 27611   GB/T 20861   SN/T 4624.20   SN/T 4624.18   SN/T 4624.10   

Basic data

Standard ID SN/T 4624.9-2016 (SN/T4624.9-2016)
Description (Translated English) Methods for examination of import microbial blends in the environmental protection��Part 9: Diarrheogenic Escherichia coli
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard Z04
Word Count Estimation 18,145
Date of Issue 2016-08-23
Date of Implementation 2017-03-01
Regulation (derived from) State-Quality-Inspection-Certification (2016)438
Issuing agency(ies) General Administration of Customs

SN/T 4624.9-2016: Methods for examination of import microbial blends in the environmental protection—Part 9: Diarrheogenic Escherichia coli


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Methods for examination of import microbial blends in the environmental protection-Part 9. Diarrheogenic Escherichia coli People's Republic of China Exit Inspection and Quarantine standards Inbound environmental testing methods for microbial agents Part 9. diarrhea caused by Escherichia coli protection-Part 9.DiarrheogenicEscherichiacoli 2016-08-23 released 2017-03-01 Implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 4624 "inbound environmental testing methods using microbial agents" is divided into 17 parts. --- Part 1. Bacillus licheniformis; --- Part 2. Bacillus pumilus; --- Part 3. Bacillus megaterium; --- Part 4. Acidithiobacillus ferrooxidans; --- Part 5. β-hemolytic streptococcus; --- Part 6. Staphylococcus aureus; --- Part 7. Salmonella; --- Part 8. Shigella; --- Part 9. Diarrhea caused by Escherichia coli; --- Part 10. Paecilomyces lilacinus; --- Part 11. Elegant small ginkgo mildew; --- Part 12. Trichoderma harzianum; --- Part 13. Phanerochaete chrysosporium; --- Part 14. Curvularia; --- Part 15. Bacillus amyloliquefaciens; --- Part 16. Alkaloid-like-producing Pseudomonas; --- Part 17. Pseudomonas putida. This section is part 9 of the SN/T 4624. This section drafted in accordance with GB/T 1.1-2009 given rules. This part of the national certification and accreditation supervision and management committee proposed and centralized. This part of the drafting unit. People's Republic of China Liaoning Exit Inspection and Quarantine. The main drafters of this section. Li Chengyu, Wang Qiuyan, Yu Li, Jiang Lingling, Kang Kai, Wang Fang. Inbound environmental testing methods for microbial agents Part 9. diarrhea caused by Escherichia coli

1 Scope

This part of SN/T 4624 specifies the morphological identification of diarrhea Escherichia coli hygienic test of microbial inoculant for entry into the environment, Biochemical identification, general PCR, real-time fluorescence PCR detection method. This section applies to inbound environmental Microbial agents in diarrhea Escherichia coli detection and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 main reagents and medium

3.1 Experimental water should be consistent with a water GB/T 6682 specifications. 3.2 Saline. See A.1 in Appendix A. 3.3 Nutritious broth. See A.2. 3.4 intestinal bacteria bacteria broth see A.3. 3.5 MacConkey Agar. See A.4. 3.6 Eosin Blue agar. See A.5. 3.7 trisaccharide iron agar. See A.6. 3.8 Cladisilide iron agar See A.7. 3.9 Sugar fermentation tubes (lactose, rhamnose, xylose and mannitol). See A.8. 3.10 Lysine decarboxylase test medium. See A.9. 3.11 Urea agar. See A.10. 3.12 Potassium cyanide See A.11. 3.13 peptone water, indigo reagent see A.12. 3.14 Semi-solid agar See A.13. 3.15 Oxidase Reagents. See A.14. 3.16 Gram staining solution See A.15. 3.17 pathogenic Escherichia coli diagnosis of serum, invasive diagnosis of Escherichia coli serum, enterotoxigenic Escherichia coli diagnosis of blood Qing, hemorrhagic Escherichia coli diagnosis of serum. 3.18 bacterial genomic DNA extraction kit. 3.19 TE buffer (pH 8.0). See A.16. 3.20 Lysozyme (10 mg/mL). See A.17. 3.21 Proteinase K (20 mg/mL). See A.18. 3.22 10% SDS. See A.19.

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