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SN/T 2660-2010: Preservation methods for culture collection in foodstuff microbiology laboratory
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Preservation methods for culture collection in foodstuff microbiology laboratory
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SN/T 2660-2010
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Basic data | Standard ID | SN/T 2660-2010 (SN/T2660-2010) | | Description (Translated English) | Preservation methods for culture collection in foodstuff microbiology laboratory | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 36,391 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB 19489; pathogenic microbiology laboratory bio- safety regulations; human infectious pathogenic microorganisms list; animal pathogenic microorganisms Category List | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the food microbiology laboratory culture collections of commonly used definitions, technical principles, technical requirements and technical methods. This standard applies to food microbiology experiments declared strains preserved. | SN/T 2660-2010: Preservation methods for culture collection in foodstuff microbiology laboratory---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Preservation methods for culture collection in foodstuff microbiology laboratory
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Food Microbiology Laboratory Culture Collection Methods
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters. Zhang Haibin, Gao Qi Li, Zhang Xia, Liu Pei, Wangnai Fu, Zhang Haiying Wu winter snows.
Food Microbiology Laboratory Culture Collection Methods
1 Scope
This standard specifies the food microbiology laboratory common definition of culture collections, technical principles, technical requirements and technical methods.
This standard applies to food microbiology laboratory species preservation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
Pathogenic microbiology laboratory bio-safety regulations
List of pathogenic organisms transmissible between humans
Animal pathogens classified directory
3 Definitions
The following terms and definitions apply to this document.
3.1
Culture Collection culturepreservation
The microbial strains with a variety of suitable methods proper preservation, to avoid death, pollution, maintaining its original traits stable.
3.2
Regular transplantation periodicalsubculturing
Also known as subculture preservation law, including slant culture, stab culture, liquid culture and so on. The inoculated in a suitable medium, optimum
Cultured under conditions, sufficient to be grown after save at 4 ℃ ~ 6 ℃ certain time interval and a transplantation of cultured Culture Collection
method.
3.3
Nujol preservationinliquidparaffin
Preservation method, also known as mineral oil, regular transplant preservation adjunct law. The inoculated slant on a suitable medium, in optimum conditions
After cultured to grow healthy bacteria colonies inject sterile liquid paraffin, it covers the entire slope, and then placed upright in a low temperature (4 ℃ ~
6 ℃) dry place to save a species preservation methods.
3.4
Porcelain beads preservation method porousbeadspreservation
The cultured microbial cells or spores made good suspension, into the porous containing sterile glass beads (or ceramic beads) in a sterile vial to suck
Glass beads attached to the surface, removal of one strain preservation method excess suspension, Cryopreservation.
3.5
Freeze drying preservation method of freeze-drying
The principle is the microbial cells in the frozen, reduced pressure, the occurrence of "sublimation" dehydration, cell physiological activity tends to stop, long-term maintenance
Viable state.
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