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                    Specifications for oceanographic survey -- Part 6: Marine biological survey
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  Basic data             |  Standard ID  |          GB/T 12763.6-2007 (GB/T12763.6-2007) |               |  Description (Translated English)  |          Specifications for oceanographic survey -- Part 6: Marine biological survey |               |  Sector / Industry  |          National Standard (Recommended) |               |  Classification of Chinese Standard  |          A45 |               |  Classification of International Standard  |          07.060 |               |  Word Count Estimation  |          169,147 |               |  Date of Issue  |          2007-08-13 |               |  Date of Implementation  |          2008-02-01 |               |  Older Standard (superseded by this standard)  |          GB/T 12763.6-1991 |               |  Quoted Standard  |          GB/T 14014; GB/T 12763.1; GB/T 12763.7; GB 17378.7 |               |  Regulation (derived from)  |          China Announcement of Newly Approved National Standards No. 8, 2007 (No. 108 overall) |               |  Issuing agency(ies)  |          General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |               |  Summary  |          This standard specifies the general requirements of marine biological investigations, technical requirements and investigation (measured) elements, sampling, sample analysis and data compilation of the basic requirements and methods. This section applies to the basic elements of the marine environment in marine survey survey.  |         
  GB/T 12763.6-2007: Specifications for oceanographic survey -- Part 6: Marine biological survey---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.  
Specifications for oceanographic survey Part 6. Marine biological survey
ICS 07.060
A45
National Standards of People's Republic of China
Replacing GB/T 12763.6-1991
Oceanographic survey
Part 6. Marine Biological Survey
Posted 2007-08-13
2008-02-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Table of Contents
Preface Ⅶ
1 Scope 1
2 Normative references 1
3 Terms and definitions
3 4 General provisions
4.1 Technical Design 3
4.2 Survey Requirements 3
4.3 Investigation and analysis equipment 4
4.4 Sample 4
4.5 Sample Analysis 5
4.6 data compilation and report preparation 5
5 Determination of chlorophyll, primary productivity and new production of 6
5.1 Determination of technical requirements and features 6
5.2 seawater phytoplankton pigments measuring 6
5.3 marine primary productivity (14C tracer method) 10
5.4 size of chlorophyll a and primary productivity grade determination 11
5.5 new marine productivity (15N tracer method) 12
5.6 data compilation 14
18 6 microorganisms survey
6.1 Technical requirements and investigation elements 18
6.2 Sampling 19
19 6.3 Sample Analysis
6.4 data compilation 29
7 pico, micro and small plankton surveys 30
7.1 Technical requirements and investigation elements 30
7.2 Sampling 30
7.3 Sample Analysis 32
7.4 data compilation 33
8 large and medium-sized plankton survey 34
8.1 Technical requirements and investigation elements 34
8.2 Sampling 35
36 8.3 Sample Analysis
8.4 data compilation 37
9 38 ICHTHYOPLANKTON survey
9.1 Technical requirements and investigation elements 38
9.2 Sampling 38
40 9.3 Sample Analysis
9.4 data compilation 40
10 large benthic surveys 41
10.1 Technical requirements and investigation elements 41
10.2 Sampling 41
43 10.3 Sample Analysis
10.4 data compilation 44
11 meiobenthos survey 44
11.1 Technical requirements and investigation elements 44
11.2 Sampling 45
46 11.3 Sample Analysis
11.4 data compilation 48
Between 12 intertidal Biological Survey 48
12.1 Technical requirements and investigation elements 48
12.2 Sampling 49
51 12.3 Sample Analysis
12.4 data compilation 51
13 52 Survey of fouling organisms
13.1 Technical requirements and investigation elements 52
13.2 Sampling 52
54 13.3 Sample Analysis
13.4 data compilation 55
14 nekton surveys 56
14.1 Technical requirements and investigation elements 56
14.2 Sampling 57
58 14.3 Sample Analysis
14.4 data compilation 61
Appendix A (normative) microorganism most probable number (MPN) retrieval table 63 and counting
Appendix B (normative) sample number plankton biomass measurement, counting fish and plankton nets 66
Annex C (normative) macrofauna fixative preparation, sample number and underwater photographic and video 71
Annex D (normative) meiobenthos sample separation, and several computing equipment 73
Appendix E (normative) intertidal biological surveys and several tidal measurement apparatus of FIG. 79
Annex F (normative) swimming animal gonad maturity, feeding intensity, fat content and trawl type 83
Annex G (informative) acoustic surveys and fishery resources assessment (OPTIONAL) 89
Appendix H (informative) Marine Biological Survey Sampling and analysis record sheet format 98
References 158
Figure B. 1 pair of drum net Figure 69
Figure B. 2 North Pacific Network 70
Figure D. 1 meiobenthos stars like structure government side schematic view and the lateral view 76
Figure D. 2 Schematic diagram of washing sandy samples closure 76
Figure D. 3 small ice Separating biological schematic 77
Figure D. 4 different size conversion factor of 77 copepods
Figure D. 5 divers carry matching scuba (SCUBA) devices such as schematic 78
Figure E. 1 tide level curve graphically curve 80
Figure E. 2 direct measurement curve 80
Figure E. 3a) covering an area of 80 count box
Figure E. 3b) Rock quantitative sampling frame 80
Figure E. 4 beach quantitative sampling frame 81
Figure E. 5 whirlpool sorter 81
Figure E. 6 sieved 82
Figure F. 1 pair of winged boat bottom trawl Type A single capsule 86
Figure F. Two pairs of winged ship bottom trawl single capsule type B 87
Figure F. 3 single-winged single boat trawl bag 88
Figure G. 1 bottom trawl survey dedicated FIG. 95
Figure G. 2 survey dedicated Water Trawl nets 96
Figure G. 3 acoustic instrument calibration standard target schematic 97 suspension
Table 1 water harvesting level 3
Table 2 HPLC gradient elution 10
Table 3 pico, micro and small plankton sampling aqueous layer vertical segment 30
Table 4 micro and small plankton nets specifications and application objects 31
Table 5 medium-sized plankton sampling aqueous layer vertical segment 34
Table 6 medium-sized plankton nets specifications and application objects 35
Table 7 fish and plankton nets specifications Applicable 39
Table 8 test plate type, size and quantity 53
Table A. 1 most probable number (MPN) counting (15) the number of bacteria retrieval table 63
Table B. 1 pair of drum configuration and network specifications 69
Table B. 2 North Pacific network configuration and specifications 70
Table D. 1 conversion factor meiobenthos table 74
Table F. 1 pair of winged unicystic boat bottom trawl nets type B, paragraph 7 to paragraph 24 of the knitting Size 87
Table G. 1 fish target strength and length relationship between the reference value of 94
Table H. 1 chlorophyll sampling record sheet 98
Table H. 2 chlorophyll (extracted fluorescence) measurement record table 99
Table H. 3 chlorophyll (Spectrophotometric Method) record table 100
Table H. 4 chlorophyll (HPLC) Determination record table 101
Table H. 5 primary productivity sampling, filtering, measurement recording table 102
Table H. 6 new production sampling, filtering, measurement recording table 103
Table H. 7 microbial sampling site record table 104
Table H. 8 marine waters viral, bacterial count of the number of direct recording table 105
Table H. 9 bacterial cell sizer record table 106
Table H. 10 water samples, soil samples and counting microbial culture isolates record table 107
Table H. 11 water samples bacterial productivity record table 108
Table H. 12 different bacteria in water samples Determination of record feed table 109
Table H. 13 samples of bacteria most probable number (MPN) record table 110
Table H. 14 sea plankton sample record table 111
Table H. 15 vertical stratification of plankton trawl sampling record table 112
Table H. 16 plankton samples registration table 113
Table H. 17 pico-cell photosynthetic plankton record number table 114
Table H. 18 micro and small plankton specimen count the number of records table 115
Table H. 19 water harvesting plankton count the number of sample record table 116
Table H. 20 phytoplankton (cysts) cell record table 117
Table H. 21 zooplankton volume fraction measurement record table 118
Table H. 22 zooplankton biomass wet weight measurement record table 119
Table H. 23 zooplankton biomass dry weight measurement record table 120
Table H. 24 zooplankton individual record count table 121
Table H. 25 Noctiluca individual record count table 122
Table H. 26 fish at sea plankton collected record table 123
Table H. 27 124 ICHTHYOPLANKTON specimens registration form
Table H. 28 ICHTHYOPLANKTON record count table 125
Table H. 29 Number of fish plankton tables 126
Table H. 30 large sea benthic sampling record table 127
Table H. 31 large-scale quantitative analysis of benthic record table 128
Table H. Qualitative analysis of 32 large benthic record table 129
Table H. 33 large-scale quantitative analysis of benthic species distribution record table 130
Table H. Qualitative analysis of 34 large benthic species distribution record table 131
Table H. 35 meiobenthos sea sampling record table 132
Table H. 36 meiobenthos quantitative analysis recording table 1133
Table H. 37 meiobenthos quantitative analysis log table 2134
Table H. 38 meiobenthos qualitative analysis table 135 records
Table H. Room 39 intertidal biological wild collection record table 136
Table H. 40 intertidal zone between quantitative analysis record table 137
Table H. 41 intertidal zone between the qualitative analysis record table 138
Table H. Room 42 intertidal species distribution table 139
Table H. Intertidal zone between the 43 main species vertical distribution table 140
Table H. 44 intertidal zone between tables 141
Table H. 45 micro-fouling organisms recording table 142
Table H. 46 Ship fouling record table 143
Table H. 47 buoy fouling record table 144
Table H. 48 docks, piling fouling record table 145
Table H. 49 Analysis of fouling organisms recording table 146
Table H. 50 fouling species record table 147
Table H. 51 nekton trawls card 148
Table H. 52 fish bioassays record table 149
Table H. 53 shrimp bioassays record table 150
Table H. 54 crabs biological assays record table 151
Table H. 55 foot class biological assays record table 152
Table H. 56 The number of animals swim tables 153
Table H. Determination of 57 fish from long tables 154
Table H. 58 fish weight measuring tables 155
Table H. 59 fish fecundity record table 156
Table H. 60 157 acoustic surveys observation record sheet
Foreword
GB/T 12763 "oceanographic survey" is divided into 11 parts.
--- Part 1. General;
--- Part 2. oceanographic observations;
--- Part 3. Marine meteorological observations;
--- Part 4. Marine Chemistry Elements investigation;
--- Part 5. Ocean Sound, light elements investigation;
--- Part 6. Marine Biological Survey;
--- Part 7. Marine survey data exchange;
--- Part 8. Marine Geological Geophysical Survey;
--- Part 9. Marine Ecological Survey Guidelines;
--- Part 10. seabed topography survey;
--- Part 11. Marine Engineering Geological Survey.
Wherein Section 9, Section 10 and Section 11 corresponds to GB/T 12763-1991 is a new section.
This section GB/T 612 763 Part of, and replaces GB/T 12763.6-1991 "specification for oceanographic survey marine life
survey".
This section compared with the GB/T 12763.6-1991 The main changes are as follows.
--- Adjust the general framework of this section, an increase of "survey among 12 intertidal zone" between normative appendix "intertidal biological survey tide
Level measurement method and apparatus several map "(see Appendix E) and information appendix" acoustic surveys and fishery resources assessments "(see Appendix G); the
1991 edition of the normative appendix "Seawater chlorophyll a, b, c of (Spectrophotometric Method)" "5 Leaf Green moved revised
Su, primary productivity and new production of Determination "(1991 edition of Appendix A; this edition 5.2.2); the original," Part IV
Plankton investigation "modified broken down into" 7 pico, micro and small plankton surveys "," 8 large and medium-sized plankton
SURVEY ON "and" 9 ICHTHYOPLANKTON investigation "three chapters, including" 7 pico, micro and small plankton surveys "for the new
Research project;
--- "2 Normative references", increasing the GB 17378.7 specification for marine monitoring Part 7. Ecological survey of offshore pollution
And biological monitoring;
--- "3 Terms and definitions", add and modify the related terms (1991 version 3.4 and 3.7; Excerpts of 3.2, 3.3,
3.4,3.5,3.8,3.9,3.10 and 3.11); at the same time each term are supplemented with the corresponding English name;
--- "4 general provisions", the survey projects and major equipment additions are two new items and new test equipment
(1991 version 5.1 and 6; edition 4.2.1 and 4.3); increased to 200m deep in this edition of "Table 1 mining water level" in the
Mining water level (see 4.2.4.1); further provides a number of investigations, detailed investigation quarter time (1991 version
5.5.2; 4.2.5 edition); modify and extend the scope of the laboratory (1991 version 6.2.1, this version 4.3.2);
According to this revised. provision "oceanographic survey - Part 1 General" GB/T 12763.1-2007, the voyage report
And the person in charge prepare a revised technical report on the survey results from the original respectively by the chief scientist and project leader chaired
(1991 version 9.2.1 and 9.2.2, this version of 4.6.1.4 and 4.6.1.5) and the like;
--- In "5 of chlorophyll, primary productivity and new production determination", the increase of the "high-performance liquid chromatography (HPLC) method," "chlorophyll a
And the size of the primary productivity of classifying determination "and" new marine productivity (15N tracer method) "(see 5.2.3,5.4 and 5.5);
--- In the "6 microbial survey", adding "SYBRGreenI direct counting method", "DAPI direct counting method", "bacterial volume
Determination "," 3H- leucine tracer method "Determination of bacterial production of" ecological respiration rate determination "," bacterial specific growth rate, doubling time and
Calculation of generation time "and" classification and identification of microorganisms "(see 6.3.4.1.1.1,6.3.4.1.1.3,6.3.4.1.1.4,
6.3.4.2.1.2,6.3.4.2.2.2,6.3.4.3 and 6.3.5);
--- A new chapter "7 pico, micro and small plankton investigation";
--- In the "8 large and medium-sized plankton investigation", increasing the gear type, modified continuous observation frequency, sample handling, sample ID
And mapping the value standard (1991 version 20.1.2,19.6,20.3.4,21.1 and 22.6; Excerpts 8.2.1.1,8.1.1. 3,
8.2.3.2,8.3.1 and 8.4.4);
--- In the "9 ICHTHYOPLANKTON investigation", modify the collection nets abundance of formulas (1991 version 20.1.2 and 22.4;
Excerpts of 9.2.1.1 and 9.4.1);
--- "10 large benthic survey", adding "deep drag of the optical system and deep-sea benthic trawl" and "macrofauna
Undersea photography, video and diving sampling "(see 10.2.1.2.5 and Appendix C.4);
--- In the "11 small benthic survey", adding "diving sampling", "intertidal collected core samples," "sol Ludox-TM from
Heart floating method "Diversity and multivariate statistical analysis and biological community structure (see 11.2.1.6 and 11.2.2.2,11.2.2.1,
11.3.4 and Appendix D. 4); modified sampling equipment (1991 version 28.1; edition 11.2.1);
--- "Between 12 intertidal Biological Survey" for the new chapter;
--- In the "13 fouling organisms investigation", modify the contents of the technical requirements (1991 version 31; Excerpts 13.1.1), an increase of tune
Charles elements and cement test panels for fouling survey Coast Port Engineering Construction (13.1.2 and 13.2.1.1.1c));
--- In the "14 animals swim investigation", the "GSI" and "full feeding factor" into the "data compilation" (1991 edition
The 37.3.1.5 and 37.3.1.6; Excerpts 14.4.2.4 and 14.4.2.5). Revised and added some important catches
Gonad maturity and fish fat content of the criteria for the classification (see Appendix Excerpts F.1.2, Appendix F.1.3, Appendix F.1.4, Appendix
F. 1.6, Appendix F. 1.7, Appendix F. 1.8 and Appendix F. 3); modified necton trawl type (1991 edition Appendix F7;
Excerpts of Appendix F. 4);
--- Modify and increase the informative appendix "Census of Marine Life, tabular analysis records" (1991 edition Appendix G; Excerpts
Appendix H).
--- This section should be GB/T 12763.1 and GB/T 12763.7 supporting the use.
This section of the Appendix A, Appendix B, Appendix C, Appendix D, Annex E and Annex F are normative appendices, Appendix G and Appendix H of data
Appendix.
This part by the State Oceanic Administration.
This part is administered by the National Oceanic Standards and Metrology Center.
This part by the Third Institute of Oceanography, State Oceanic Administration responsible for revising, First Institute of Oceanography, State Oceanic Administration, State Oceanic Administration, Second Marine
Institute, Ocean University of China, Chinese Academy of Fishery Sciences East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute
Participate amendment.
Drafting and revision of the main people in this section. Zhang Yusheng, Yang Qingliang, Chen Ruixiang, Zhu Mingyuan, Ip Tak Chan, Ningxiu Ren, Lin Jinghong, OCEANOGRAPHY
Jiang Jinxiang, Zhi-Nan, Li Rongguan, Huangzong Guo, Zhengcheng Xing Zheng Yuan A, Zhaoxian Yong, Lv Ruihua, Lu bucket set, Shijun Xian Cai Yu Ming, Lin and Zhou.
This part replaces the standard release case.
--- GB/T 12763.6-1991.
Oceanographic survey
Part 6. Marine Biological Survey
1 Scope
GB/T 12763 provisions of this part of the general provisions of the Census of Marine Life, technical requirements and survey (measurement) elements, sampling, sample
Basic requirements and methods of analysis and data compilation.
This section applies to the marine environment, the basic elements of the Census of Marine Life Survey.
2 Normative references
The following documents contain provisions which, through GB/T 12763 reference in this text, constitute provisions of this part. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB/T 14014 silk, synthetic fiber mesh
GB/T 12763.1 specification for oceanographic survey - Part 1. General
GB/T 12763.7 specification for oceanographic survey - Part 7. Marine survey data processing
GB 17378.7 specification for marine monitoring Part 7. Ecological survey of offshore pollution and biological monitoring
3 Terms and Definitions
GB/T 15919 and established the following terms and definitions apply GB/T 12763 in this section.
3.1
Autotrophic plant cells, an important class of pigments, which absorb and transfer light energy major substance plant photosynthesis.
Chlorophyll a (Chla) is one of the main pigment.
3.2
Autotrophic organisms produce organic matter through photosynthesis ability. Usually in unit time (days or years) per unit area (or volume) in the production
Health organics (generally represented organic carbon) mass, equivalent to the time within the same area (or volume) of the primary production.
[GB/T 15919-1995, the definition 2.210]
3.3
It means the photosynthetic efficiency of plants photosynthetic pigments. In CO2 and adequate illumination conditions, and the same unit mass of chlorophyll per hour
The ratio of the amount of carbon [conventional "carbon (mg)/chlorophyll (mg)/hr" indicates].
[GB/T 15919-1995, the definition 2.217]
3.4
In the euphotic recirculating nitrogen regeneration nitrogen, nitrogen provided by outside euphotic for the new nitrogen. First by the support that part of the regeneration of the nitrogen source
Productivity known as reclaimed productivity level, supported by the nascent nitrogen that part called the new production of primary productivity.
   
   
  
  
    
  
    
   
        
       
          
               
 
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