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GB 23200.54-2016: Food safety national standard -- Determination of residual methacrylic acid ester fungicides in foodstuffs by gas chromatography-mass spectrometry
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Food safety national standard -- Determination of residual methacrylic acid ester fungicides in foodstuffs by gas chromatography-mass spectrometry
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GB 23200.54-2016
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Basic data | Standard ID | GB 23200.54-2016 (GB23200.54-2016) | | Description (Translated English) | Food safety national standard -- Determination of residual methacrylic acid ester fungicides in foodstuffs by gas chromatography-mass spectrometry | | Sector / Industry | National Standard | | Classification of Chinese Standard | G25 | | Word Count Estimation | 17,150 | | Date of Issue | 2016-12-18 | | Date of Implementation | 2017-06-18 | | Older Standard (superseded by this standard) | SN/T 2237-2008 | | Regulation (derived from) | State Health Commission, Ministry of Agriculture, Food and Drug Administration Notice No. 16 of 2016 | | Issuing agency(ies) | National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration | GB 23200.54-2016: Food safety national standard -- Determination of residual methacrylic acid ester fungicides in foodstuffs by gas chromatography-mass spectrometry
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Food safety national standard - Determination of residues methacrylic acid ester fungicide residues in foodstuffs by gas chromatography - mass spectrometry
National Standard of the People 's Republic of China
GB
Instead of SN/T 2237-2008
National standards for food safety
The amount of methacrylic acid ester fungicide in food
Determination of gas chromatography - mass spectrometry
National food safety standards-
Determination of strobilurin fungicides residues in foods
Gas chromatography-mass spectrometry
2016-12-18 release
2017-06-18 Implementation
National Health and Family Planning Commission of the People 's Republic of China
Issued by the Ministry of Agriculture of the People 's Republic of China
State Administration of Food and Drug Administration
Foreword
This standard replaces SN/T 2237-2008 "Determination of Methoxy Acrylate Fungicide Residue in Import and Export Foods Gas Chromatography -
Mass spectrometry ".
Compared with SN/T 2237-2008, the main changes are as follows.
- Standard text format is modified to national standard text format for food safety;
- the name and scope of the "export food" to "food";
- increase the "other food reference implementation" in the standard range.
This standard replaced the previous version of the standard release.
-SN/T 2237-2008.
National standards for food safety
Determination of Residues of Methoxy Acrylate Fungicides in Food
Gas chromatography - mass spectrometry
1 Scope
This standard specifies the preparation and determination of 11 kinds of methacrylic acid ester fungicide residues in foodstuffs (see Appendix A).
This standard applies to apples, pears, grapes, cabbage, cucumbers, mushrooms, orange juice, tea drinks, rice, chestnut, beef, pork, chicken
Meat, eggs and milk and other foods in the determination of 11 kinds of methacrylic acid ester fungicide residues. Including azoxystrobin, ketamine, pyrimethanone,
(Z, E), oxime oxytetracycline, picolorate, pyrazole mushroom and oxytetracycline, other food
Goods can refer to the implementation.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this
file. For undated references, the latest edition (including all modifications) applies to this document.
GB 2763 National Standard for Food Safety - Maximum Residue Limit of Pesticides in Foodstuffs
GB/T 6682 Analytical laboratory water specifications and test methods
3 principle
The samples were extracted with organic solvent and purified by gel permeation chromatography (GPC). After the eluate was concentrated and constant volume, the gas chromatographic mass spectrometer
Determination of external standard method.
4 reagents and materials
Unless otherwise specified, all reagents are of analytical grade and water is in accordance with the primary water specified in GB/T 6682.
4.1 Reagents
4.1.1 Toluene (C7H8). Chromatographic pure.
4.1.2 Cyclohexane (C6H12). Chromatographic Purification.
4.1.3 Ethyl acetate (C4H8O2). Analytical purity.
4.1.4 acetonitrile (C2H3N). pure chromatography.
4.1.5 Methanol (CH4O). Chromatographic Purification.
4.1.6 Trichloromethane (CHCl3). analytical grade.
4.1.7 Sodium chloride (NaCl), analytical grade.
4.1.8 anhydrous sodium sulfate (Na2SO4), analytical grade. Before burning at 650 ℃ for 4h, placed in a dryer after cooling and standby.
4.2 solution preparation
4.2.1 Cyclohexane-ethyl acetate (1 1, V/V). Take 100 mL of cyclohexane, add 100 mL of ethyl acetate and shake well.
4.3 standards
4.3.1 Methoxy Acrylate Fungicides Standard Substance. See Appendix A.
4.4 standard solution preparation
4.4.1 Standard stock solution. accurately weighed fungicide standard 50 mg (accurate to 0.1 mg), dissolved in toluene, transferred to 50 mL brown
Volumetric flask and constant volume to scale. The solution concentration was 1 mg/mL. -18 ℃ below the dark preservation, the shelf life of 12 months.
4.4.2 Standard Intermediate. Draw 1.00 mL. Standard stock solution, move into 100 ml. Brown volumetric flask, with cyclohexane - ethyl acetate volume,
The solution concentration was 10 μg/mL. 0 ℃ ~ 4 ℃ dark preservation, the shelf life of 1 month.
4.4.3 standard working fluid. absorb a certain amount of standard intermediate, with cyclohexane - ethyl acetate diluted to the required concentration, the use of pre-preparation.
5 instruments and equipment.
5.1 Gas Chromatography Mass Spectrometer (GC-MS) with an electronically bombarded ion source (EI source).
5.2 Gel Chromatography Purification System (GPC).
5.3 Analysis of balance. 0.01 g and 0.0001 g.
5.4 Homogenizer. Maximum speed 20 000 r/min.
5.5 Centrifuge. Maximum speed 5 000 r/min.
5.6 Vortex Mixer.
5.7 chicken heart bottles. 100 mL.
5.8 Plastic centrifuge tube. 50 mL.
5.9 nitrogen blowing instrument.
6 Preparation and storage of samples
6.1 Preparation of the sample
6.1.1 Fruits and vegetables
Extract about 500 g of fruit such as apple, mushroom, cucumber and other fruits or vegetables, cut it, and then use the crusher to process the sample into a slurry.
Uniform, divided into two copies as a sample, into a clean sample bottle, sealed and marked.
6.1.2 Grains and nuts
Extract rice and other nuts and chestnuts and other nut samples about 500 g, and then crushed by crusher and through the 2.0 mm round hole sieve, mix,
Are divided into two as a sample, into the clean Sheng Sheng bag, sealed and marked.
6.1.3 meat and animal offal
Extraction of beef, chicken, pork and other samples of about 500 g, chopped, with a crusher to sample processed into a slurry, mix, are divided into two
As a sample, into a clean vial, sealed and marked.
6.1.4 Eggs
Take the sample of about 500 g of eggs, peel the shell, and use the crusher to process the sample into a slurry, mix it, and divide it into two pieces.
Clean the vials, sealed and marked.
6.1.5 Beverages and milk
Take about 500 g of the beverage and milk samples, mix them into two pieces, and separate them into a clean sample vial.
mark.
Note. The above sample sampling site according to GB 2763 Appendix A implementation.
6.2 Sample storage
Cereals, nuts and other samples stored at 0 ℃ ~ 4 ℃; fresh fruits, vegetables and meat, eggs, milk and beverages and other samples at -18 ℃
The following frozen storage.
During the operation of the sample preparation, the sample should be protected from contamination or changes in the content of the residue.
7 Analysis steps
7.1 Extraction
7.1.1 samples of fruits, vegetables and beverages
The prepared samples were thawed at room temperature and left for about 30 min. Weigh 15 g of the sample (accurate to 0.01 g) at 50 mL
Plastic centrifuge tube, add 5g sodium chloride, vortex mixed 1 min, add 30 ml cyclohexane - ethyl acetate, and then vortex mixed 1 min, super
Sonic extraction for 10 min, centrifuged at 4 000 r/min for 10 min. Take the supernatant to fill the funnel with 5 g of anhydrous sodium sulfate
20 mL of the filtrate (equivalent to 10 g of sample) into the heart of the bottle. 40 ℃ below the rotation concentrated to near dry.
7.1.2 Grain samples, nuts samples
The prepared sample was removed and allowed to stand at room temperature for about 30 min. Weigh 15 g of the sample (accurate to 0.01 g) at 50 mL. plastic
Centrifuge tube, add 5g sodium chloride, then add 30 ml of acetonitrile, vortex mixing 1 min, ultrasonic extraction 30 min, to 4 000 r/min
Centrifuge for 10 min. Take the supernatant filled with 2g anhydrous sodium sulfate funnel, take 20 ml of filtrate (equivalent to 10 g sample) into the
Heart of the bottle. 40 ℃ below the rotation concentrated to near dry.
7.1.3 Meat and animal offal
The prepared samples were thawed at room temperature and left for about 30 min. Accurately weigh 15 g of the sample (accurate to 0.01 g)
50 mL of plastic centrifuge tube, add 5 g sodium chloride vortex mixed 1 min, standing 10 min, then add 30 mL of acetonitrile, ultrasonic extraction 30 min,
Centrifuge at 4 000 r/min for 10 min. Take the supernatant filled with 5g anhydrous sodium sulfate funnel, take 20 ml of filtrate (equivalent to
10 g sample) into the heart of the bottle. 40 ℃ below the rotation concentrated to near dry.
7.1.4 eggs, milk samples
The prepared samples were thawed at room temperature and left for about 30 min. Accurately weigh 10 g of the sample (accurate to 0.01 g)
50 ml of plastic centrifuge tube, add 5 ml of methanol, about 10 g of anhydrous sodium sulfate and 20 ml of chloroform, vortex mixing 1min, oscillation
20 min, centrifuged at 4 000 r/min for 10 min. The lower layer of chloroform was taken with a pipette and the residue was filled with 5 g of anhydrous sodium sulfate
Bucket, collected in the heart of the chicken. Add 20 ml of trichloromethane and repeat the above steps for secondary extraction. Rinse the funnel with 20 ml of acetonitrile and water
Sodium sulfate several times. Combined filtrate in the heart of the bottle, 40 ℃ below the water bath under reduced pressure to near dry.
7.2 Purification
7.2.1 The residue in the heart of the heart was dissolved in 4 mL of cyclohexane-ethyl acetate and transferred to a GPC vial for GPC purification.
7.2.2 GPC conditions. Bio beads (S-X3) gel purification column, the mobile phase of cyclohexane ethyl acetate, flow rate 4 mL/min, detection wave
Long 254 nm. The injection volume was 2 mL. The eluate was collected from the 15 min to 20 min period, or the elution curve was taken according to the elution curve
Determine the collection time period.
7.2.3 Concentration. The eluate collected in the heart of the heart was concentrated below 40 ° C to near dryness.
7.2.4 Dissolution. Dissolve the residue in the heart of the heart with 1.0 mL of cyclohexane-ethyl acetate and transfer to a GC vial for GC-MS detection.
7.3 Determination
7.3.1 Gas Chromatographic Analysis Reference Conditions
A) Column. HP-5 ms quartz capillary column, 30 m × 0.25 mm (id), film thickness 0.25 μm, or equivalent;
B) Column temperature program. 160 ° C for 1 min, rise to 280 ° C at 15 ° C/min for 4 min at 10 ° C/min
Rose to 300 ° C for 10 min.
C) Carrier gas flow rate. 0.8 mL/min for 10 min, 0.5 mL/min to 1.5 mL/min for 12 min;
D) Inlet temperature. 280 ° C.
E) mass spectrometer interface temperature. 280 ℃.
F) Carrier gas. high purity helium, purity ≥99.999%.
G) Injection method. no split injection, 1.5 min after the opening valve.
H) Injection volume. 2.0 μL.
I) GC-MS interface temperature. 280 ° C.
7.3.2 Reference conditions for mass spectrometry analysis
A) Ionization mode. EI.
B) Ionization energy. 70 eV.
C) Quadrupole temperature. 150 ° C.
D) ion source temperature. 230 ° C.
E) Detection mode. Select ion mode (SIM).
F) Monitoring of ions (m/z). 11 kinds of fungicides to choose the monitoring of ions in Table 1.
G) solvent delay. 6 min.
Table 1 11 kinds of fungicide retention time, qualitative and quantitative ions
No. pesticide name
Reference retention time
/ Min
Quantitative ions
M/z
Qualitative ion
M/z
Qualitative ion 2
M/z
Qualitative ion 3
M/z
1 picolinate 7.99 335.1 303.1 173.1 145.1
2 E-phenoxylate 8.17 238.1 191.1 167.1 284.1
3 ether bacteria Ester 8.37 206.1 131.1 116.1 313.2
4 Z-phenoxylate 8.45 196 191 238 345.9
5 pyrimidone ester 8.89 189.1 204.1 426.2 352.1
6 oxime strain 9.14 131.1 116.1 172.1 377.2
7 ether bacteria 9.88 295.2 16.1 237.2 not selected
8 oxime ether amide 10.23 205.2 132.1 221.1 116.1
9 pyrazole mushroom 11.41 325.0 132.0 164.0 not selected
10 fluoxibenolide 13.15 363.3 313.1 306.1 398.2
11 Azoxystrobin 14.90 344 372.1 388.1 403.2
7.3.3 Qualitative determination
Under the above conditions, the reference retention time of 11 kinds of fungicides was about 7.9 min-15 min (see Table 1). standard
Product Selection Ion Monitoring GC-MS Figure See Appendix A, Figure A.1.
When the sample solution is measured according to the conditions determined by gas chromatography mass spectrometry, if the detected retention time of the chromatographic peaks is different from that in the standard solution
And all selected ions are present in the sample mass spectrum after subtracting the background, and the selected ion abundance is proportional to the standard
Of the ion abundance ratio within the allowable error range (see Table 2), you can determine the presence of such fungicides in Xiang products. If it can not be confirmed, should be heavy
The new injection is further confirmed by means of scanning (if sufficient sensitivity) or by the addition of other confirmed ions.
Table 2 The maximum allowable deviation of relative ion abundance when qualitative confirmation
Relative abundance (base) 50% 20% to 50% 10% to 20% ≤10%
Allowable relative deviation ± 20% ± 25% ± 30% ± 50%
7.3.4 Quantitative determination
Quantitative determination using external standard method. According to the content of fungicides, select the peak area similar to the standard working solution together for chromatography
Spectrum Analysis. The response values of the standard working solution and the fungicide in the sample to be measured should be within the linear range of the test. For standard working solutions and to be tested
Sample volume and other volume into the plug sample.
7.4 blank experiment
Unless the sample is not weighed, according to the above steps.
8 results are calculated and expressed
(1) to calculate the content of each fungicide in the sample by gas chromatography mass spectrometer workstation processing software,
X = error did not find the reference source. (1)
Where.
X - the content of the fungicide in the sample in milligrams per kilogram, mg/kg;
A - the chromatographic peak area of the fungicide in the sample solution;
Ao - the peak area of the fungicide in the blank experiment;
As - the chromatographic peak area of the fungicide in the standard solution;
Cs - the concentration of the fungicide in the standard solution, in micrograms per milliliter, μg/mL;
V - the final volume of the sample solution, in milliliters (mL);
M - the amount of sample represented by the final sample, in grams, g.
Note. The result of the calculation shall be deducted from the blank value. The result of the measurement shall be expressed as the arithmetic mean of the parallel measurement, and two valid digits shall be retained.
9 precision
9.1 The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with
Appendix D requirements.
9.2 The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with
Appendix E requirements.
10% limit and recovery rate
10.1 Quantitation limits
In this method, the limit of the 11 fungicides in different matrices is given in Appendix B.
10.2 Recovery rate
In this method, the recovery rates of 11 fungicides in various food matrices are given in Appendix C.
Appendix A
(Informative)
Eleven methacrylic acid ester fungicides and GC-MS
Table A.1 Molecular formula, molecular weight and American Chemical Abstracts for eleven methacrylic acid ester fungicides
Serial number
Pesticide Chinese
name
Pesticide English
name
CAS number Molecular formula Molecular weight retention time min
Monitor the ion abundance ratio
1 azoxystrobin 131860-33-8 C22H17N13O5 403.12 14.90
344, 372.1, 388.1, 403.2
2 ether bacteria
Dimoxystrobi
141961-52-4 C19H22N2O3 326.16 9.88
295.2, 116.1, 237.2
3 pyrimethanone fluacrypyrim 229977-93-9 C20H21F3N2O5 426.14 8.89
189.1, 204.1, 426.2, 352.1
4 fluoxastrobin fluoxastrobin 361377-29-9
C21H16CIFN4
O5
458.08 13.15
363.3, 313.1, 306.1, 398.2
5 ether bacteria
Kresoxim-me
Thyl
143390-89-0 C18H19NO4 313.13 8.37
206.1, 131.1, 116.1, 313.2
Z-phenoxy bacteria
Z-metominos
Trobin
133408-51-2 C16H16N2O3 284.12 8.45
196,191,238,345.9
E-phenoxy bacteria
E-metominos
Trobin
133408-50-1 C16H16N2O3 284.12 8.17
238.1, 191.1, 167.1, 284.1
8 oxime ether bacteria orysastrobin 248593-16-0 C18H25N5O5 405.2 10.23
205.2, 132.1, 221.1, 116.1
9 picolinate
Picoxystrobi
248593-16-0 C18H25N5O5 367.1 7.99
335.1, 303.1, 175.1, 145.1
Pyrazole bacteria
Pyraclostrobi
175013-18-0
C19H18CIN3O
387.82 11.41
325.0, 182.0, 164.0
11 oxime ester
Trifloxystrobi
142017-21-7 C20H19F3N2O4 408.13 9.14
131.1, 116.1, 172.1, 377.2
Note."
"Marked ions are quantitative ions
1 - picolamate; 7 -
2 - E - phenoxamine; 8 - oxime;
3 - ether bacteria; 9-1 - pyrazole bacteria 1);
4 - Z - phenoxybenzamine; 10 - fluoxazole;
5 - pyrimidium ester; 9-2 - pyrazole bacteria;
6 - oxime; 11 - azoxystrobin
Figure A.1 Eleven kinds of methacrylic acid ester fungicide standard gas chromatography-mass spectrometry total ion current
1) The GC-MS of the pyrazole bacteria showed bimodal (9-1, 9-2), the area of the two peaks under different concentration range and different chromatographic conditions
The proportions are different. When the pyrazole bacteria is determined, the selective ion abundance ratio of the two peaks can be used as a qualitative basis,
Under the conditions of the standard solution of the actual situation of the peak to choose a peak for the set. This standard selects the first peak during the experimental study
For quantitative basis.
Appendix B
(Informative)
The quantitative data of the sample is limited
Table B.1 Experimental data on the quantitative limits of the sample
Pesticide limit (mg/kg)
Picolinate
Bacteria
E-phenoxy
Fungi
Ether bacteria Z-phenoxy
Fungi
Pyrimethanone oxime fungus ether ketone oxime ether bacteria
Pyrazole
Ether bacteria
Fluorouracil
Bacteria
Azoxystrobin
Apple 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.050 0.010
Grape 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Pear 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Cabbage 0.010 0.005 0.005 0.010 0.005 0.05 0.008 0.005 0.010 0.005 0.010
Cucumber 0.010 0.005 0.05 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Mushroom 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Orange juice 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
green tea
Drink
0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Chestnut 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Hair 0.010 0.005 0.005 0.010 0.005 0.005 0.008 0.005 0.010 0.005 0.010
Chicken 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Pork 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Beef 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Eggs 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Milk 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Appendix C
(Informative)
Sample concentration and recovery of the experimental data
Table C.1 Recovery range of eleven fungicides in fruit and vegetable substrates
No. Pesticide Name Added Level/(mg/kg)
Recovery rate/%
Pear Mushroom Orange Juice Green Tea Drink Apple Grape Brassica
1 picolinate
0.01 87.56-104.17 72.70-94.70 80.39-87.01 82.69-109.77 73.15-101.85 78.31-111.62 80.46-109.08 80.92-112.15
0.02 79.14-97.68 72.50-96.35 79.79-98.48 88.38-98.23 72.35-98.12 76.15-101.96 73.00-94.27 72.69-94.38
0.06 71.87-100.88 86.77-104.29 70.96-99.62 83.26-99.05 82.31-104.28 74.65-102.28 76.25-100.06 78.65-95.97
E-phenoxy
Fungi
Lt;/RTI & gt; 88.53
Gt;
0.06 86.17-106.76 81.90-114.94 88.33-98.33 82.03-98.42 77.43-98.57 77.43-94.17 79.10-98.70 75.77-98.92
3 ether bacteria
0.01 83.44-108.73 81.02-96.84 88.62-96.35 81.23-111.92 78.69-99.38 78.69-107.08 81.08-111.69 80.77-104.23
0.02 87.21-97.65 88.81-101.66 88.12-98.66 81.69-99.42 91.96-101.23 81.23-101.62 79.85-100.96 80.92-103.27
0.06 88.83-102.75 78.57-104.52 87.89-101.66 83.83-103.12 78.49-96.54 78.49-92.88 79.88-102.55 79.88-102.08
Z-phenoxy
Fungi
Gt;
0.05 76.43-96.03 76.36-91.97 76.36-95.95 88.44-98.63 76.42-92.04 79.21-104.79 88.54-105.69 84.15-95.35
0.1 85.69-101.48 79.17-106.52 84.69-100.30 88.30-96.17 79.17-98.23 79.30-87.73 71.17-86.87 71.17-86.87
5 pyrimidium ester
0.01 93.50-108.14 84.56-106.47 84.56-116.47 80.10-118.40 85.60-106.20 76.20-95.60 80.70-113.60 82.70-115.60
0.02 89.14-100.20 88.50-105.40 91.85-103.25 80.30-98.40 85.35-104.80 77.25-93.85 77.45-96.20 72.20-96.00
0.05 95.85-102.10 81.23-112.43 96.60-102.90 88.08-103.98 81.22-98.02 81.22-98.02 83.98-99.02 80.08-99.98
Oxime ester
0.01 88.13-115.00 99.45-112.85 93.81-112.85 70.82-114.36 99.45-112.85 68.64-113.18 73.27-117.73 70.82-113.55
0.02 95.89-119.76 90.16-104.27 97.05-120.88 87.09-101.82 87.95-106.27 84.23-104.09 84.09-102.18 79.32-101.82
0.05 94.99-108.12 74.36-95.76 93.80-106.76 82.24-105.00 74.27-92.15 83.05-90.64 74.27-90.16 74.47-100.42
7 ether amine
0.02 91.12-115.60 81.20-102.08 81.20-102.08 83.88-109.24 81.41-102.00 84.24-99.06 83.53-110.82 83.88-109.24
0.09 95.99
0.08 94.33-102.12 85.21-105.47 94.00-101.76 83.12-100.76 90.76-105.52 85.28-95.08 86.35-97.44 83.94-98.14
8 oxime ether bacteria
0.01 91.22-101.44 80.20-97.36 80.20-97.36 72.50-111.60 80.40-97.60 70.20-91.50 77.20-117.00 77.50-110.70
0.02 96.03-103.21 86.05-95.65 90.10-97.00 80.80-97.65 76.80-95.50 76.80-95.70 73.50-99.20 72.85-97.65
0.05 91.22-106.87 73.95-99.46 90.90-106.50 84.42-97.08 73.52-99.56 77.86-96.82 75.68-97.81 77.08-96.82
9 pyrazole bacteria
0.02 90.17-105.70 91.40-108.40 91.40-103.20 82.14-110.50 81.48-99.22 81.48-17.08 83.52-111.00 83.40-110.10
0.05 90.09-96.98 86.20-105.14 84.40-103.98 83.37-96.02 80.35-102.30 80.35-98.35 86.29-98.08 77.77-98.23
0.08
10 fluoxazole ester
0.05 88.68-111.16 81.46-99.36 81.46-99.36 76.89-105.56 91.78-110.18 92.09-105.11 83.51-106.67 81.33-110.00
0.1 78.51-103.54 96.03-102.33 97.83-101.74 92.02-104.16 84.87-99.96...
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