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WS/T 693-2020: (Guide to mosquito biological control technology Bacterial larvicides)
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(Guide to mosquito biological control technology Bacterial larvicides)
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WS/T 693-2020
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Basic data | Standard ID | WS/T 693-2020 (WS/T693-2020) | | Description (Translated English) | (Guide to mosquito biological control technology Bacterial larvicides) | | Sector / Industry | Health Industry Standard (Recommended) | | Classification of Chinese Standard | C05 | | Word Count Estimation | 6,666 | | Date of Issue | 2020-08-17 | | Date of Implementation | 2021-02-01 | | Regulation (derived from) | State-health communication (2020) No. 17 | | Issuing agency(ies) | National Health Commission | WS/T 693-2020: (Guide to mosquito biological control technology Bacterial larvicides)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technique guide of bio-control for mosquito--Bacterial larvicide
ICS 11.020
C 05
WS
People's Republic of China Health Industry Standard
Guide to Mosquito Biological Control Technology Bacterial Larvicides
2020-08-17 released
2021-02-01 implementation
Issued by the National Health Commission of the People's Republic of China
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Drafting organizations of this standard. Institute of Microbiology and Epidemiology, Academy of Military Medicine, Chinese Academy of Military Sciences, Beijing Municipality
Prevention and Control Center, Jiangsu Provincial Center for Disease Control and Prevention.
The main drafters of this standard. Zhao Tongyan, Dong Yande, Li Chunxiao, Zeng Xiaopeng, Tong Ying, Zhang Yong, Chu Hongliang.
Guide to Mosquito Biological Control Technology Bacterial Larvicides
1 Scope
This standard specifies the principle of using bacterial larvicides (Bacillus thuringiensis subsp. Israel and Bacillus sphaericus) to control mosquito larvae,
Insecticidal spectrum, formulation, application method and effect evaluation.
This standard applies to the use of bacterial insecticides (Bacillus thuringiensis subsp. Israel and Bacillus sphaericus) to control mosquito larvae.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document.
For undated references, the latest version (including all amendments) applies to this document.
GB/T 23797 Vector density monitoring method for mosquitoes
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Bacillus thuringiensis Israel subspecies Bacillusthuringiensis·israelensis; Bti
Belongs to gram-positive bacilli, which produce insecticidal parasporal crystal protein called delta-endotoxin during the sporulation process.
Mosquito larvae such as Mosquitoes and Anopheles have stomach poisoning effects and are a kind of bacterial insecticide.
3.2
Bacillus sphaericus; Bs
It belongs to aerobic bacillus that forms subterminal expanded sporangia and spherical spores. 9 of the 49 flagellar serotypes have been found
Strains of type (H1, H2, H3, H5, H6, H9, H25, H26 and H48) have a certain toxic effect on mosquito larvae and are a kind of bacterial killing
Insect repellent.
3.3
Biological activity unit
It is the unit of biologically active substance of Bacillus thuringiensis subsp. Israel and Bacillus sphaericus, expressed as ITU/mg.
4 Principle of action of bacterial juvenile
4.1 Bacillus thuringiensis subsp. Israel
The parasporal crystals of Bacillus thuringiensis subsp. Israel are degraded by the alkaline environment in the midgut of mosquito larvae and released by enzymatic hydrolysis of intestinal proteases
Toxic peptides are released, paralyzing the larvae, destroying the potassium ion (K) balance in the mosquito larvae and poisoning to death.
4.2 Bacillus sphaericus
The toxic effect of Bacillus sphaericus on different mosquito larvae is mainly realized by the toxin protein it produces. It has been proven that in its growth
Two types of toxin proteins can be produced during the breeding process, one is the crystal toxin protein that exists in all highly virulent strains; the other is
Low-virulence strains neutralize the Mtx toxin protein in some high-virulence strains.
5 Insecticidal spectrum of bacterial juveniles
5.1 Bacillus thuringiensis subspecies Israel is the most toxic to Aedes larvae. The toxic effect to Culex larvae is second, and the toxic effect to Anopheles larvae
The effect is poor.
5.2 Bacillus sphaericus has a relatively narrow mosquito-killing spectrum. It has a good toxic effect on the larvae of Culex and Anopheles, but it has a good effect on the larvae of Aedes.
The effect is poor.
6 dosage forms
6.1 Bacillus thuringiensis subsp. Israel
6.1.1 Suspending agent
Applicable to various water bodies, suitable for open water bodies and water bodies without aquatic vegetation.
6.1.2 Water dispersible granules
This formulation can be used to spray large areas of liquid on typical hidden larval breeding sites in open water bodies, water bodies with little or no aquatic vegetation.
fog. Directly control water accumulation in artificial or natural containers. For the types of surface activities such as Anopheles, in actual operation, the treatment is repeated
The timing is 4th instar larvae.
6.1.3 Wettable powder
This type of dosage form has a variety of biologically active units prepared, and water should be mixed before use.
6.1.4 Granules
This type of dosage form has a variety of substrates, and has the characteristics of being able to release Bacillus thuringiensis subspecies Israel into the water to the maximum extent. Granule bleaching
The shift is smaller, it can target the most targets, and has a better effect of penetrating water vegetation than liquid spray.
6.1.5 Block
The biologically active unit of this type of dosage form varies with the manufacturer and technical matrix, and generally depends on the type of block and the biological activity that can release Su
Bacillus aureus subsp. Israel is 30 days to 180 days, and its effect will not be changed due to moisture and dryness. For both permanent and temporary breeding grounds
Suitable for. The block has a ring shape and usually floats, and a brick shape usually sinks to the bottom. This type of formulation is less effective in open water, and the wind may blow it
To the edge. Therefore, it is generally used in small water bodies, such as ponds, pools, and sinks. Blocking agent is easy to form lumps in sewage, so it should be used
Relatively clean water.
6.2 Bacillus sphaericus
The dosage forms that can be used include granules (generally bioactive unit is 50 BsITU/mg), water dispersible granules (generally labeled bio
The unit of material activity is 650 BsITU/mg). Suspending agents, blocks and water-soluble granules are also used.
6.3 Mixing agent
The dosage form mixed with Bacillus thuringiensis subsp. Israel and Bacillus sphaericus can be used in the breeding grounds of all larvae.
7 Application method
7.1 Bacillus thuringiensis species Israel
7.1.1 Liquid spray
Suspending agents, water dispersible granules, and wettable powders can be used for liquid spraying. The spray speed depends on the type, efficacy,
Vector mosquito species and breeding grounds. The suspension can be used diluted or undiluted, and the treatment interval is determined according to the volume of the water body and the coverage area of the breeding ground
(7 d ~ 14 d). Water dispersible granules are usually mixed with water before use. For artificial or natural containers, use them directly without mixing water beforehand.
The treatment interval is 7 to 14 days, and the Aedes mosquitoes are controlled in the container. The treatment interval is 3 months. Wettable powders need to be in water for all water bodies
Medium mixing, interval treatment is generally 7 d to 14 d. All dosage forms can use manual compression pumps or other sprayers, aiming at world health
The recommended dosage of Bacillus thuringiensis water dispersion recommended by the organization is 125 g/ha～750 g/ha for open water and 1 mg～5 mg/L for container.
7.1.2 Spreading granules
Manually or with a portable blower to release into the water body, according to the World Health Organization recommended dose of Bacillus thuringiensis, open water recommendations
The dosage is 5 kg/ha～20 kg/ha, and the interval treatment is generally 7 d～14 d.
7.1.3 Blocking agent
The floating ring-shaped block agent is manually released (10 ITU/m2) and needs to be fixed on plants or other objects with a rope to avoid wind interference.
No special treatment is required for the brick block agent to be put into the water bottom.
7.2 Bacillus sphaericus
7.2.1 Liquid spray
Suspending agents and water-dispersible granules can be used for liquid spraying, and the spraying speed depends on the type of dosage form, efficiency, vector mosquito species and breeding place.
The suspension can be used diluted or undiluted, depending on the volume of the water body and the coverage of the breeding ground. Water dispersible granules are usually mixed before use
Water, the preparation should be thoroughly mixed for 48 h to 72 h before use. Generally effective for 28 days, and the treatment frequency depends on the target mosquito species, breeding ground conditions and retention
The effect is determined by the time limit. See Appendix A for the dosage used.
7.2.2 Spreading granules
Release into the water body by manual or portable blower. The effective period of the preparation is generally 21 to 35 days, depending on the target mosquito species and breeding
The time of re-administration is determined by local conditions and retention time. Refer to Appendix A for dosage.
8 Effect evaluation
Before and after the use of Bacillus thuringiensis subsp. Israel and Bacillus sphaericus to control mosquito larvae, it should be carried out in accordance with the GB/T 23797 standard
The density of larvae is monitored, and the rate of density decrease is calculated to evaluate the control effect.
Appendix A
(Informative appendix)
Reference dosage of Bacillus thuringiensis subsp. Israel and Bacillus sphaericus
Table A.1 shows the reference dosage of Bacillus thuringiensis subsp. Israel and Bacillus sphaericus as larvicides.
Table A.1 Reference dosage of Bacillus thuringiensis subsp. Israel and Bacillus sphaericus
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