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SN/T 4631-2016: Detection and identification of Narcissus common latent virus
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| SN/T 4631-2016 | English | 329 |
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Detection and identification of Narcissus common latent virus
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SN/T 4631-2016
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Basic data | Standard ID | SN/T 4631-2016 (SN/T4631-2016) | | Description (Translated English) | Detection and identification of Narcissus common latent virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,177 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4631-2016: Detection and identification of Narcissus common latent virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Narcissus common latent virus
People's Republic of China Exit Inspection and Quarantine standards
Narcissus common latent virus quarantine and identification methods
2016-08-23 released
2017-03-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted by Fujian Entry-Exit Inspection and Quarantine of People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of the People's Republic of China
Bureau, Institute of Biotechnology, Chinese Academy of Agricultural Sciences, China Academy of Inspection and Quarantine, Zhejiang Academy of Inspection and Quarantine Science and Technology, Fujian
Agriculture and Forestry University.
The main drafters of this standard. Shen Jianguo, Liao Fu Rong, Li Min, Li Weimin, Zhang Yongjiang, Shao Biying, Liu Zhengcai, Wu Ying, Gao Fangluan, Wu Zujian.
Narcissus common latent virus quarantine and identification methods
1 Scope
This standard specifies the Narcissus common latent virus quarantine methods.
This standard applies to immigration Narcissus scales corm, plants Narcissus common latent virus detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
SN/T 2122 Quarantine and sampling methods for entry and exit of plants and plant products
SN/T 2964 plant virus detection specification
SN/T 3457 Codex specification for plant virus molecular biology
3 principle
The serological and molecular biological properties of Narcissus common latent virus (see Appendix A for background information) are the main
To be based.
4 equipment, appliances and reagents
4.1 Equipment
High-speed refrigerated centrifuge, electronic balance (1/1000g), PCR instrument, electrophoresis apparatus, horizontal electrophoresis tank, -20 ℃ low temperature refrigerator and -80 ℃
Ultra-low temperature refrigerator, gel imaging analysis system, pH meter, microwave oven, magnetic stirrer, constant temperature water bath, autoclave, clean bench, enzyme
Marker and so on.
4.2 appliances
Adjustable pipettes of various ranges (1000 μL,.200 μL, 100 μL, 20 μL, 10 μL, 2 μL), PCR tubes, RNase-free
Eppendorf centrifuge tube (1.5 mL), mortar, ELISA plate and the like.
4.3 Reagents
Unless otherwise specified, all reagents are of analytical grade, the experimental water should be consistent with the relevant provisions of GB/T 6682. ELISA plate capture antigen
See Appendix B for plate-trapped antigen ELISA (PTA-ELISA) reagents, see RT-PCR Reagents see
Appendix C, real-time fluorescence RT-PCR detection reagents see Appendix D.
5 Sampling and sample preparation
5.1 Sampling
Try to extract the virus damage the symptoms of materials, NCLV hazards described in Appendix A; Sampling methods in accordance with SN/T 2122
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