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SN/T 4632-2016 English PDF

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SN/T 4632-2016: Detection and identification of Zucchini yellow mosaic virus
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Basic data

Standard ID SN/T 4632-2016 (SN/T4632-2016)
Description (Translated English) Detection and identification of Zucchini yellow mosaic virus
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Word Count Estimation 14,177
Date of Issue 2016-08-23
Date of Implementation 2017-03-01
Regulation (derived from) State-Quality-Inspection-Certification (2016)438
Issuing agency(ies) General Administration of Customs

SN/T 4632-2016: Detection and identification of Zucchini yellow mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Zucchini yellow mosaic virus China's entry-exit inspection and quarantine industry standards Small squash yellow mosaic virus quarantine and identification method Published on.2016-08-23 2017-03-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued Inspection and Quarantine of the People's Republic of China Industry Standard Small squash yellow mosaic virus quarantine and identification method China Standard Press Publishing December.2017 first edition

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by the People's Republic of China. Henan Entry-Exit Inspection and Quarantine Bureau and Xiamen Entry-Exit Inspection and Quarantine Bureau, Guangxi University, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences. Drafters of this standard. Zhao Zhongyu, Han Ying, Li Meng, Song Nan, Liao Furong, Zhu Yingzhi, Peng Faqing, Dai Yuhua, Wu Huijie. Small squash yellow mosaic virus quarantine and identification method

1 Scope

This standard specifies the quarantine and identification methods for the small zucchini yellow mosaic virus. This standard applies to inward and outward seeds, seedlings, tissue culture seedlings and other plant-based propagation materials and fresh or preserved fruits in small zucchini yellow mosaic disease Quarantine identification of poisons.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. SN/T 1840 Plant Virus Immunoelectron Microscopy Assay SN/T 2122 Sampling methods for quarantine of entry and exit plants and plant products

3 Basic Information of Zucchini Yellow Mosaic Virus

Scientific name. Zucchiniyellowmosaicvirus Abbreviation. ZYMV Taxonomic status. Potyviridae, Potyvirus See Appendix A for additional information.

4 Methodology

The serological and molecular biological characteristics of Zucchini yellow mosaic virus are the main basis for quarantine and identification.

5 Equipment and main reagents

5.1 Instruments and Equipment Enzyme-Linked Detector, Balance (Sense. 1/10000g), pH Meter (Measurement Accuracy. ±0.05pH), Micro Juice Extractor, High Speed Refrigerated Centrifuge Machine, plant light incubator (temperature adjustable range 0°C~50°C), isolation greenhouse, transmission electron microscope (magnification..200,000 times), PCR Instrument, electrophoresis apparatus, electrophoresis tank, gel imaging system, constant temperature water bath, low temperature refrigerator, etc. 5.2 Appliances Micropipettes (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL), enzyme-linked plates, mortar, eppendorf tube, copper mesh (400 meshes), dropper, tipped tweezers, Whatman filter paper, plastic petri dish, clean glass sheet, Parafilm, flowerpot, sterilized soil, and the like. 5.3 Main Reagents Enzyme-linked immunosorbent assay reagents (see Appendix B), RT-PCR detection reagents (see Appendix C).

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