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SN/T 4624.15-2016 English PDF

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SN/T 4624.15-2016: Methods for examination of import microbial blends in the environmental protection—Part 15: Bacillus amyloliquifaciens
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PDF similar to SN/T 4624.15-2016


Standard similar to SN/T 4624.15-2016

HG/T 5871   GB/T 27611   GB/T 20861   SN/T 4624.20   SN/T 4624.18   SN/T 4624.10   

Basic data

Standard ID SN/T 4624.15-2016 (SN/T4624.15-2016)
Description (Translated English) Methods for examination of import microbial blends in the environmental protection��Part 15: Bacillus amyloliquifaciens
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard Z04
Word Count Estimation 14,148
Date of Issue 2016-08-23
Date of Implementation 2017-03-01
Regulation (derived from) State-Quality-Inspection-Certification (2016)438
Issuing agency(ies) General Administration of Customs

SN/T 4624.15-2016: Methods for examination of import microbial blends in the environmental protection—Part 15: Bacillus amyloliquifaciens


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Methods for examination of import microbial blends in the environmental protection-Part 15. Bacillus amyloliquifaciens People's Republic of China Exit Inspection and Quarantine standards Inbound environmental testing methods for microbial agents Part 15. Bacillus amyloliquefaciens protection-Part 15.Bacillus amyloliquifaciens 2016-08-23 released 2017-03-01 Implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released Exit Inspection and Quarantine of People's Republic of China Industry Standard Inbound environmental testing methods for microbial agents Part 15. Bacillus amyloliquefaciens China Standard Press First edition of November.2017

Foreword

SN/T 4624 "inbound environmental testing methods using microbial agents" is divided into 17 parts. --- Part 1. Bacillus licheniformis; --- Part 2. Bacillus pumilus; --- Part 3. Bacillus megaterium; --- Part 4. Acidithiobacillus ferrooxidans; --- Part 5. β-hemolytic streptococcus; --- Part 6. Staphylococcus aureus; --- Part 7. Salmonella; --- Part 8. Shigella; --- Part 9. Diarrhea caused by Escherichia coli; --- Part 10. Paecilomyces lilacinus; --- Part 11. Elegant small ginkgo mildew; --- Part 12. Trichoderma harzianum; --- Part 13. Phanerochaete chrysosporium; --- Part 14. Curvularia; --- Part 15. Bacillus amyloliquefaciens; --- Part 16. Alkaloid-like-producing Pseudomonas; --- Part 17. Pseudomonas putida. This section is part 15 of SN/T 4624. This section drafted in accordance with GB/T 1.1-2009 given rules. This part of the national certification and accreditation supervision and management committee proposed and centralized. This part of the drafting unit. People's Republic of China Liaoning Exit Inspection and Quarantine. The drafters of this section. Jia Jinsheng, Zhou Jiannan, Zhang Xinyuan, Wang Jinling, Zhang Ying, Wang Fang, Li Shu. Inbound environmental testing methods for microbial agents Part 15. Bacillus amyloliquefaciens

1 Scope

This part of SN/T 4624 specifies the morphological identification of Bacillus amyloliquefaciens in conformity with the inoculation microbial inoculum Identification, molecular biology detection methods. This section applies to the entry of environmental Microbacterium agent for the test of Bacillus amyloliquefaciens consistency test and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 main reagents and medium

3.1 Experimental water should be consistent with a water GB/T 6682 specifications. 3.2 Nutrient agar. See Appendix A, A.1. 3.3 Nutritious broth. See A.2. 3.4 Gram staining related reagents. See A.3. 3.5 Contact enzymes. See A.4. 3.6 Oxidase. See A.5. 3.7 Sugar fermentation tube. See A.6. 3.8 Citrate. See A.7. 3.9 Nitrate reduction See A.8. 3.10 Starch hydrolysis See A.9. 3.11 Gelatin See A.10. 3.12 TE buffer (pH 8.0). See A.11. 3.13 1% to 3% agarose gel, see A.12. 3.14 25 mmol/LEDTA. See A.13. 3.15 3 mol/L sodium acetate. See A.14. 3.16 dNTP.dATP, dTTP, dGTP, dCTP. 3.17 TBE. See A.15. 3.18 Taq DNA polymerase. 3.19 bacterial genomic DNA extraction kit. 3.20 agarose. 3.21 ethidium bromide. 3.22 DNA molecular weight marker .100bp DNAladder.

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