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SN/T 4624.13-2016: Methods for examination of import microbial blends in the environmental protection—Part 13: Phanerochaete chrysosporium
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4624.13-2016 | English | 229 |
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Methods for examination of import microbial blends in the environmental protection—Part 13: Phanerochaete chrysosporium
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SN/T 4624.13-2016
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Basic data | Standard ID | SN/T 4624.13-2016 (SN/T4624.13-2016) | | Description (Translated English) | Methods for examination of import microbial blends in the environmental protection��Part 13: Phanerochaete chrysosporium | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Z04 | | Word Count Estimation | 10,128 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4624.13-2016: Methods for examination of import microbial blends in the environmental protection—Part 13: Phanerochaete chrysosporium
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Methods for examination of import microbial blends in the environmental protection-Part 13. Phanerochaete chrysosporium
People's Republic of China Exit Inspection and Quarantine standards
Inbound environmental testing methods for microbial agents
Part 13. Phanerochaete chrysosporium
protection-Part 13.Phanerochaetechrysosporium
2016-08-23 released
2017-03-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Exit Inspection and Quarantine of People's Republic of China
Industry Standard
Inbound environmental testing methods for microbial agents
Part 13. Phanerochaete chrysosporium
China Standard Press
First edition of November.2017
Foreword
SN/T 4624 "inbound environmental testing methods using microbial agents" is divided into 17 parts.
--- Part 1. Bacillus licheniformis;
--- Part 2. Bacillus pumilus;
--- Part 3. Bacillus megaterium;
--- Part 4. Acidithiobacillus ferrooxidans;
--- Part 5. β-hemolytic streptococcus;
--- Part 6. Staphylococcus aureus;
--- Part 7. Salmonella;
--- Part 8. Shigella;
--- Part 9. Diarrhea caused by Escherichia coli;
--- Part 10. Paecilomyces lilacinus;
--- Part 11. Elegant small ginkgo mildew;
--- Part 12. Trichoderma harzianum;
--- Part 13. Phanerochaete chrysosporium;
--- Part 14. Curvularia;
--- Part 15. Bacillus amyloliquefaciens;
--- Part 16. Alkaloid-like-producing Pseudomonas;
--- Part 17. Pseudomonas putida.
This section is part 13 of the SN/T 4624.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This part of the national certification and accreditation supervision and management committee proposed and centralized.
This part of the drafting unit. People's Republic of China Liaoning Exit Inspection and Quarantine.
The main drafters of this section. Li Junhuan, Li Shu, Fu Haibin, Wu Miao Miao, Wang Fang, Jia Jinsheng, Lee Sung-chan.
Inbound environmental testing methods for microbial agents
Part 13. Phanerochaete chrysosporium
1 Scope
SN/T 4624 provisions of this part of the entry of environmental Microbacterium compatibility test the morphology of Phanerochaete chrysosporium morphological identification,
PCR detection method.
This section applies to the entry of environmental Microbiological Agents compliance test of Phanerochaete chrysosporium detection and identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 main reagents and medium
3.1 Experimental water should be consistent with a water GB/T 6682 specifications.
3.2 Potato Dextrose Agar See Appendix A, A.1.
3.3 Potato Dextrose Broth See A.2.
3.4 CTAB buffer. See A.3.
3.5 Protease K.
3.6 Tris saturated phenol.
3.7 trichloromethane.
3.8 Isoamyl alcohol.
3.9 isopropanol.
3.10 TE buffer (pH 8.0). See A.4.
3.11 10 × PCR buffer,.200 mmol/L Tris-HCl (pH 8.4),.200 mmol/L KCl 15 mmol/L Magnesium chloride
(MgCl2).
3.12 dNTP.dATP, dTTP, dGTP, dCTP.
3.13 TBE.54gTris, 27.5g boric acid, dissolved in 800mL deionized water, 20mL0.5mol/LEDTA (pH8.0) fixed volume to 1L.
3.14 Taq DNA Polymerase.
3.15 Fungal genomic DNA extraction kit.
3.16 50 × TAE buffer (pH 8.5). See A.5.
3.17 agarose.
3.18 ethidium bromide.
3.19 DNA molecular weight marker .100bp DNAladder.
3.20 liquid nitrogen.
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