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SN/T 3767.4-2014: Loop-mediated isothermal amplification detection method for genetically modified components in food for export. Part 4: MaizeBt 176
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| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 3767.4-2014 | English | 239 |
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Loop-mediated isothermal amplification detection method for genetically modified components in food for export. Part 4: MaizeBt 176
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Basic data
| Standard ID | SN/T 3767.4-2014 (SN/T3767.4-2014) |
| Description (Translated English) | Loop-mediated isothermal amplification detection method for genetically modified components in food for export. Part 4: MaizeBt 176 |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | X04 |
| Classification of International Standard | 67.050 |
| Word Count Estimation | 9,940 |
| Quoted Standard | GB/T 6682; GB/T 19495.2; GB/T 19495.3; GB/T 19495.5; GB/T 19495.7; SN/T 3767.2-2014 |
| Regulation (derived from) | AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This Standard specifies the food transgenic maize Bt176 strain-specific loop-mediated isothermal amplification (LAMP) screening test methods. This section applies to the corn and processed products transit qualitative detection of gene maize Bt176 strain- |
SN/T 3767.4-2014: Loop-mediated isothermal amplification detection method for genetically modified components in food for export. Part 4: MaizeBt 176
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
. Loop-mediated isothermal amplification detection method for genetically modified components in food for export Part 4. MaizeBt 176
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Genetically Modified food exports loop-mediated
Isothermal amplification (LAMP) detection method
Part 4. Bt176 maize lines
Part 4. MaizeBt176
Issued on. 2014-01-13
2014-08-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 3767 "genetically modified food for export loop-mediated isothermal amplification (LAMP) detection method" is divided into 30 parts.
Part 1. General requirements and definitions;
Part 2. The screening method;
Part 3. maize Bt-11 strain;
Part 4. Bt176 maize lines;
Part 5. GA21 maize lines;
Part 6. MIR162 Maize Lines;
Part 7. MIR604 maize lines;
Part 8. maize MON810 strain;
Part 9. maize MON863 strain;
Part 10. maize MON88017 strain;
Part 11. maize MON89034 strain;
Part 12. Corn T-25 strain;
Part 13. 3272 maize lines;
Part 14. Lines of corn 59122;
Part 15. A2704-12 soybean lines;
Part 16. Soybean A5547-127 lines;
Part 17. Soybean DP356043 lines;
Part 18. Soybean GTS40-3-2 lines;
Part 19. Soybean MON89788 strain;
Part 20. Rice Bt-63 strain;
Part 21. Rice KF6 lines;
Part 22. Rice KF8 lines;
Part 23. KMD rice strains;
Part 24. Rice LLRICE62 lines;
Part 25. Rice M12 strain;
Part 26. Rice T1C-19 strain;
Part 27. Rice T2A-1 strain;
Part 28. Lines wheat B73-6-1
Part 29. beet H7-1 strain;
Part 30. Rape RT-73 strain.
This section SN/T Section 43767 of.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Shantou People's Republic of China
Bureau, People's Republic of China Beijing Entry-Exit Inspection and Quarantine People's Republic of China Shanghai, China Inspection and Quarantine Bureau
Graduate School, People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Tianjin People's Republic of China, the Chinese people
Republic of Zhuhai CIQ, Zhongkai University of Agriculture and Engineering, Guangzhou Di Australian biotechnology company.
The main drafters of this section. east micro, Ling Li, Liu Jin, Cai Ying, Yi Min Ying, Xie force, Zeng Jing, Li Xiaohong, Chen Ying, Cao occasion Juan, Kit Cheng,
Wang Feng, high Su Juan, Li Zhiyong, Shi Lei, Guan Li Jun, Zhang Jun, Chen Yuan tree, Li Ting.
Genetically Modified food exports loop-mediated
Isothermal amplification (LAMP) detection method
Part 4. Bt176 maize lines
1 Scope
SN/T 3767 provisions of this part of the food of transgenic corn Bt176 strain specific loop-mediated isothermal amplification (LAMP) screening
Detection method.
This section applies to corn and its processed products transit qualitative detection of gene maize Bt176 strain-specific components.
Qualitative detection limit of this method was 0.5% (mass fraction).
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 19495.2 technical requirements for GMO testing laboratories
GB/T 19495.3 GMO detection of nucleic acid extraction and purification method
GB/T 19495.5 GMO quantitative PCR method for detecting nucleic acid
GB/T 19495.7 GMO detection methods of sampling and sample preparation
SN/T 3767.2-2014 exports of genetically modified foods loop-mediated isothermal amplification (LAMP) detection methods - Part 2. Sieve
The method of election
3 anti-pollution measures
Loop-mediated isothermal amplification detection process of pollution prevention measures should be consistent with the provisions of GB/T 19495.2 of.
4 Sampling and sample preparation
Accordance with the provisions of GB/T 19495.7 of.
5 nucleic acid extraction and purification
Accordance with the provisions of GB/T 19495.3 of.
6 LAMP detection method
6.1 Principle
6.1.1 General principles
According to internal and corn border sequences exogenous gene transgenic maize lines Bt176 design, the ring of each primer pair specific target

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