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SN/T 3137-2012 English PDF

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SN/T 3137-2012: Determination of deoxynivalenol, 3-acetyl-deoxynivalenol, 15-O-acetyl-4-deoxynivalenol and their metabolite in food for export. LC-MS/MS method
Status: Valid
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SN/T 3137-2012English629 Add to Cart 5 days [Need to translate] Determination of deoxynivalenol, 3-acetyl-deoxynivalenol, 15-O-acetyl-4-deoxynivalenol and their metabolite in food for export. LC-MS/MS method Valid SN/T 3137-2012

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Basic data

Standard ID SN/T 3137-2012 (SN/T3137-2012)
Description (Translated English) Determination of deoxynivalenol, 3-acetyl-deoxynivalenol, 15-O-acetyl-4-deoxynivalenol and their metabolite in food for export. LC-MS/MS method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 24,212
Regulation (derived from) National Quality Inspection (2012) 237
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the export of food Deoxynivalenol DON 3 acetyl Deoxynivalenol DON and 15 deoxy snow acetyl DON and its metabolite epoxy Deoxynivalenol DON residual liquid chromatography mass spectrometry/mass spectrometry. This standard applies to

SN/T 3137-2012: Determination of deoxynivalenol, 3-acetyl-deoxynivalenol, 15-O-acetyl-4-deoxynivalenol and their metabolite in food for export. LC-MS/MS method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of deoxynivalenol, 3-acetyl-deoxynivalenol, 15-O-acetyl-4-deoxynivalenol and their metabolite in food for export.LC-MS/MS method People's Republic of China Entry-Exit Inspection and Quarantine Standards Food exports deoxynivalenol fungi alcohol, 3-acetyl Deoxynivalenol fungi alcohol, 15-acetyl deoxynivalenol Determination of Fusarium alcohol and its metabolites Liquid chromatography - mass spectrometry/mass spectrometry Determinationofdeoxynivalenol, 3-acetyl-deoxynivalenol, LC-MS/MSmethod Issued on. 2012-05-07 2012-11-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shaanxi Exit Inspection and Quarantine, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Zou Yang, Li Jianhua, Ouyang Shan Yue Zhenfeng, love mountain music, Kong Xianghong, HE Qiang, Zhang Ying, Zhang Lu. Food exports deoxynivalenol fungi alcohol, 3-acetyl Deoxynivalenol fungi alcohol, 15-acetyl deoxynivalenol Determination of Fusarium alcohol and its metabolites Liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard provides the food for export deoxynivalenol fungi alcohol, 3-acetyl deoxynivalenol fungi alcohol, 15-acetyl deoxynivalenol sickle Enol knife bacteria and their metabolites to epoxy deoxynivalenol bacteria alcohol residues LC - MS/MS detection method. This standard applies to wheat, rice, corn, beer, biscuits deoxynivalenol fungi alcohol, 3-acetyl deoxynivalenol fungi alcohol, 15- acetyl deoxynivalenol bacteria alcohol and chicken, liver, kidney, milk, eggs metabolites to epoxy deoxynivalenol fungi alcohol residues Determination of the amount of the stay and confirmed.

2 Method summary

Sample of deoxynivalenol fungi alcohol, 3-acetyl deoxynivalenol fungi alcohol, 15-acetyl deoxynivalenol bacteria enolase and their representatives Epoxide metabolite go deoxynivalenol fungi alcohol extracted with acetonitrile, defatted with hexane and after solid phase extraction (SPE), liquid chromatography - mass Spectrum/MS detection, external standard.

3 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water for ultra-pure water. 3.1 acetonitrile. HPLC grade. 3.2 Methanol. HPLC grade. 3.3 hexane. pesticide grade. 3.4 sodium chloride. 3.5 anhydrous sodium sulfate. After burning 650 ℃ 4h, placed in a desiccator. 3.6 acetonitrile saturated n-hexane solution. n-hexane in the amount of 200mL 250mL separating funnel, adding a small amount of acetonitrile, shaken vigorously for a few minutes Bell, still hierarchical, discarding the lower acetonitrile layer, that is, too. 3.7 methanol - water. Measure 20mL of methanol was added 80mL of water, mix well. 3.8 standards. deoxynivalenol bacteria enolase (deoxynivalenol, DON), a purity of greater than or equal to 96.0%; 3-acetyl deoxynivalenol Fusarium enol (3-acetyldeoxynivalenol, 3-Ac-DON), a purity of greater than or equal to 94.0%, 15-acetyl deoxynivalenol bacteria ene Alcohol (15-O-acetyl-4-deoxynivalenol, 15-Ac-DON), a purity of greater than or equal to 94.0%. Standard information Table A.1. 3.9 to epoxy deoxynivalenol bacteria enolase (deepoxy-deoxynivalenol, DOM-1) in acetonitrile. a concentration of 50μg/mL. 3.10 standard stock solution. Weigh accurately were a certain amount of DON, 3-Ac-DON and 15-Ac-DON standard (3.8), dissolve with acetonitrile Solution, dubbed 0.5mg/mL standard stock solution stored in the dark at less -18 ℃. 3.11 intermediate standard solution. accurate Pipette each standard stock solution (3.9,3.10) 1mL to 10mL brown volumetric flask, constant volume with acetonitrile To the mark, to the following -18 ℃ stored. 3.12 Standard working solution. Depending on needs, accurately draw a certain amount of intermediate standard solution (3.11), were formulated with a suitable concentration of acetonitrile

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