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SN/T 2976-2011: Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction
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| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 2976-2011 | English | 239 |
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Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction
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Basic data
| Standard ID | SN/T 2976-2011 (SN/T2976-2011) |
| Description (Translated English) | Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 9,913 |
| Date of Issue | 2011-09-09 |
| Date of Implementation | 2012-04-01 |
| Quoted Standard | GB/T 6682 |
| Adopted Standard | OIE Aquatic Animal Disease Diagnostic Manual -2006, NEQ |
| Regulation (derived from) | National Quality Inspection (2011) 492; industry standard filing Notice 2012 No. 4 (No. 148 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the salmon rickettsial nested polymerase chain reaction detection methods. This standard applies to salmon rickettsial quarantine. |
SN/T 2976-2011: Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for piscirickettsia salmonis by nested polymerase chain reaction
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Salmon rickettsia quarantine Technical Specifications
Nested polymerase chain reaction
Issued on. 2011-09-09
2012-04-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard and the International Organization for Animal Health (OIE) "aquatic animal disease diagnostic manual" (2006 edition) the first chapter 2.1.13 degree of consistency
Not equivalent.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Bureau Technology Center, Shenzhen People's Republic of China
Entry-Exit Inspection and Quarantine Beijing People's Republic of China.
The main drafters of this standard. Xie star, Peng Xiaoli, Liu Tong, Chen Shuangya, Orientin Liu, Jia Peng, Zhang Lifeng, THE STAFF, Wang Qun force, Wang Jingming.
Salmon rickettsia quarantine Technical Specifications
Nested polymerase chain reaction
1 Scope
This standard specifies the salmon rickettsial nested polymerase chain reaction detection method.
This standard applies to salmon rickettsial quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
bp (basepair). bp
DNA (deoxyribonucleicacid). DNA
dNTP (deoxyribonucleosidetriphosphate). deoxynucleotide triphosphate
EDTA (ethylenediaminetetraaceticacid). ethylenediaminetetraacetic acid
Nested-PCR (nestedpolymerasechainreaction). nested polymerase chain reaction
SDS (sodiumdodecylsulfate). sodium dodecyl sulfate
Taq (Thermusaquaticu). aquatic habitat heat strain
TE. Tris-Cl, EDTA buffer
Tris [tris (hydroxymethyl) aminomethane]. tris (hydroxymethyl) aminomethane
4 Equipment and Reagents
4.1 Device
4.1.1 -20 ℃ cryogenic refrigerator.
4.1.2 tissue homogenizer.
4.1.3 High-speed refrigerated centrifuge.
4.1.4 biological safety cabinet.
4.1.5 incubator.
4.1.6 PCR instrument.
4.1.7 electrophoresis.
4.1.8 gel imager or ultraviolet light observation.
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