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SN/T 2974-2011: Quarantine protocol for bovine babesiosis
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Quarantine protocol for bovine babesiosis
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Basic data
| Standard ID | SN/T 2974-2011 (SN/T2974-2011) |
| Description (Translated English) | Quarantine protocol for bovine babesiosis |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 9,950 |
| Date of Issue | 2011-09-30 |
| Date of Implementation | 2012-04-01 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | National Quality Inspection (2011) 492; industry standard filing Notice 2012 No. 4 (No. 148 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the bovine babesiosis pathogen identification, enzyme-linked immunosorbent assay and indirect fluorescent antibody test. This standard applies to bovine babesiosis quarantine. |
SN/T 2974-2011: Quarantine protocol for bovine babesiosis
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for bovine babesiosis
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Bovine babesiosis quarantine Technical Specifications
Issued on. 2011-09-09
2012-04-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard and OIE "terrestrial animal disease diagnostic tests and vaccines Standards Manual" (2008 edition) the first chapter 2.4.2 "cow Babesia
Disease "non-equivalent.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine of Sichuan People's Republic of China
Bureau of Sichuan Agricultural University.
The main drafters of this standard. Jiang Hong, Wang Yuling, Zhao Xiangping, Yu Hua, Yangguang You, Ebel Yan, Hu Juan.
Bovine babesiosis quarantine Technical Specifications
1 Scope
This standard specifies the pathogen identification of bovine babesiosis, ELISA and indirect fluorescent antibody test.
This standard applies to bovine babesiosis quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Disease Overview
Cattle from bovine babesiosis Babesia (Babesiabovis), double bud Babesia (B.bigemina), differences Babesia (B.
divergens), etc. caused by parasitic protozoa. The disease can cause cattle fever, systemic circulatory shock, hemoglobinuria and certain neurological disorders
Like, severe can lead to death. See Appendix A.
Bovine babesiosis diagnostic methods include. pathogen identification and serology. Pathogen Identification of the traditional method is microscopy
check. This method can detect as low as 107 red blood cells infected with a parasite. Serological test include indirect immunofluorescence assay and enzyme-linked immunosorbent
Assay. Among them, the indirect immunofluorescence test has been widely used to detect Babesia antibodies, but a prominent drawback is the number of samples detected
Less, with a certain degree of subjectivity.
4 Diagnosis
4.1 microscopic examination
4.1.1 instruments and equipment
Ordinary microscope.
4.1.2 Reagents and materials
Giemsa staining solution, method of preparation see Appendix B. Test water should comply with GB/T 6682 requirements.
4.1.3 Sampling sites
Cattle are usually distributed by Babesia in blood capillaries, the best samples taken from live cattle ear tip or tail tip capillary; cattle Babesia bigemina
Adams insects and differences Babesia evenly distributed in the systemic blood, such as capillary blood collection can not make fresh smears can also be collected aseptically jugular
Vein blood anticoagulants EDTA (1mg/mL), 5 ℃ preservation, laboratory service within a few hours. Samples of dead cattle in addition to thin blood smears, should also
There are taken from the cerebral cortex, kidney, liver, lung and bone marrow tissue smears.
4.1.4 Preparation of blood smears
In clean glass slide moistened with a third of a small drop of blood to one edge smooth slide to push the film, push one end of the sheet placed in the blood drop
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