SN/T 2757: Evolution and historical versions  
      
    
  
	
		
			| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF | 
		 
			| SN/T 2757-2022 | English | RFQ | 
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                    3 days [Need to translate]
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                    (Specification for the detection of plant nematodes)
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                    SN/T 2757-2022
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			| SN/T 2757-2011 | English | 559 | 
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                    3 days [Need to translate]
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                    Rules for detection of plant parasitic nematodes
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                    SN/T 2757-2011
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			| SN/T 2757-2010 | English | RFQ | 
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                    The frontier port histoplasmosis inspection method
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                    SN/T 2757-2010
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       PDF similar to SN/T 2757-2022 
    
   
    
     
          
  
  
    
  Basic data             |  Standard ID  |          SN/T 2757-2022 (SN/T2757-2022) |               |  Description (Translated English)  |          (Specification for the detection of plant nematodes) |               |  Sector / Industry  |          Commodity Inspection Standard (Recommended) |               |  Classification of Chinese Standard  |          B16 |               |  Classification of International Standard  |          65.020.20 |               |  Date of Issue  |          2022-07-07 |               |  Date of Implementation  |          2023-02-01 |               |  Older Standard (superseded by this standard)  |          SN/T 2757-2011 |               |  Issuing agency(ies)  |          General Administration of Customs |         
  SN/T 2757-2011: Rules for detection of plant parasitic nematodes---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.  
Rules for detection of plant parasitic nematodes
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Plant nematode testing standards
Issued on. 2011-02-25
2011-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard is drafted by. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
Participated in the drafting of this standard. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, Ningbo Entry-Exit Inspection and People's Republic of China
Quarantine Bureau.
The main drafters of this standard. Li Wan, Jiang Lihui, Gu Jianfeng, gold fire Peng, Jiang Li, Li Jiang, Wangyou Fu, Wang Wanchun, Fu Haibin, Hao Hou,
Yan Chaojie.
Plant nematode testing standards
1 Scope
This standard specifies the export plant nematode testing standards.
This standard applies to the entry and exit of plants and plant products, quarantine and identification of vector insects, soil and growing media in nematodes.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 27402 laboratory quality control standards for phytosanitary
SN/T 1724 entry coniferous logs, wood and wood packaging material in the pine wood nematode quarantine procedures
SN/T 1848 plant pest identification specification
Principle 3
The use of nematodes trend aqueous and gravity, buoyancy, the nematode isolated from the host plant, soil or growing medium, packaging materials, etc.
Years, according to the morphological characteristics, combined with biology, molecular biology and other methods to determine its species.
4 appliances, instruments and reagents
4.1 Instrument
Biological microscope (100 × ~ 1000 ×), stereo microscope (10 × ~ 80 ×), incubator, low-speed centrifuge (1000r/min ~
5000r/min), clean benches, water bath, incubator, temperature electric heating plate, floating cysts, refrigerator.
4.2 Appliances
Woodworking saw or chainsaw, ax, scalpel, dissecting needle, scissors, tweezers, separation sieve (10 mesh to 20 mesh, 60 mesh to 100 mesh, 325 mesh ~
400 mesh, 500 mesh), mortar, nematodes filter paper, gauze, funnel, platter, improved Berman funnel, latex tubes, clips, straws, beakers, flasks, steamed
Fat dish or dish, slides, cover slips, slides depression, alcohol lamp, test tubes, finger-shaped tube, hole punch, dryer, marker, brush, brush, glass
Filaments, homemade nematode pick needle.
4.3 Reagents
40% formaldehyde, triethanolamine, glycerin, glacial acetic acid, a solution of sucrose (484 g Sucrose. 1L of distilled water), kaolin, paraffin, vaseline, ethanol
(Concentration of 70%, 95%, 100%), picric acid, neutral gum, nail polish, gum arabic, clove oil, 45% lactic acid, 40% hydrogen peroxide,
Potato dextrose agar, water agar.
5 Laboratory tests
5.1 Pre-treatment samples
5.1.1 Plant Tissue
The plant material (roots, stems, fruits, etc.) cut into small pieces or thin (thickness < 0.5cm), or the smaller seeds crushed plant tissue,
   
   
  
  
    
  
    
   
        
       
          
               
 
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