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SN/T 2702-2010: Quarantine protocol for swine vesicular disease
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| SN/T 2702-2010 | English | 679 |
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Quarantine protocol for swine vesicular disease
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SN/T 2702-2010
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Basic data | Standard ID | SN/T 2702-2010 (SN/T2702-2010) | | Description (Translated English) | Quarantine protocol for swine vesicular disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 26,216 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | Terrestrial animal diagnostic tests and vaccines Manual (mammals, birds and bees (OIE 2008 Edition)) | | Adopted Standard | OIE " terrestrial animal diagnostic tests and vaccines Manual (mammals, birds and bees), " 2008 edition Chapter 2.8.9, the reference | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the clinical diagnosis of swine vesicular disease, virus isolation, agar diffusion test, reverse indirect hemagglutination test, neutralization test, monoclonal antibody blocking enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction and fluorescence inverted record polymerase chain reaction procedures. This standard applies to the import and export of animals, swine vesicular disease quarantine. | SN/T 2702-2010: Quarantine protocol for swine vesicular disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for swine vesicular disease
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Swine vesicular disease quarantine Technical Specifications
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard reference to the OIE (OIE) "Terrestrial Animals Manual of diagnostic tests and vaccines (mammals, birds and bees)"
2008 Version 2.8.9 chapter.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Guangdong Entry-Exit Inspection and Quarantine
Bureau, the Shenzhen People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. salicin, sand talent, Zhu Hong thing, Liao Xiuyun, flower Qun Yi, Lin Zhixiong, Luo Qiong, Xu Nie.
Swine vesicular disease quarantine Technical Specifications
1 Scope
This standard specifies the clinical diagnosis of swine vesicular disease, virus isolation, agar diffusion test, reverse indirect hemagglutination test, neutralization test, blocking mAb
Off ELISA, reverse transcription - polymerase chain reaction procedures - polymerase chain reaction and fluorescence reverse transcription.
This standard applies to the import and export of animals swine vesicular disease quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Terrestrial animal diagnostic tests and vaccines Manual (mammals, birds and bees (OIE2008 Edition))
3 Abbreviations
The following abbreviations apply to this document.
BSL-3 (biologysafetylaboratorylevelⅢ). three laboratory biosafety
CPE (cytopathiceffect). induced cytopathic effect
When the number of cycles of each reaction tube fluorescent signal reaches the threshold set by experienced. Ct values
DEPC. Coke ethylene carbonate
ELISA (enzymelinkedimmunosorbentassay). enzyme-linked immunosorbent assay
FMDV (footandmouthdiseasevirus). FMD virus
LH (lactalbuminhydrolysate). hydrolyzed milk protein solution
PCR (polymerasechainreaction). Polymerase chain reaction
RIHA (reverseindirecthemagglutinationassay). reverse indirect hemagglutination
RT-PCR (RT-polymerasechainreaction). reverse transcription - polymerase chain reaction
SN T (serumneutralizationtest). neutralization test
SVDV (swinevesiculardiseasevirus). Swine vesicular disease virus
TCID50 (50% tissuecultureinfectivedose). half the amount of tissue infection
TTE (1,1,2-trichorotrifluoro-ethane). 1,1,2- trichlorotrifluoroethane
Fluorescent RT-PCR (fluorogenicreversetranscription-polymerasechainreaction). fluorescence reverse transcription - polymerase chain
reaction
4 clinical diagnosis
Swine vesicular disease related information, see Appendix A.
Swine vesicular disease virus (SVDV) is a small RNA virus family, enterovirus single-strand RNA viruses. Naturally infected with SVD
Only pigs and human viruses. SVD incubation period of 2d ~ 7d, a sharp rise in the incidence of swine body temperature can reach 41 ℃, less likely to cause death. blister
SVD injury is the most typical and most representative of visible pathological changes, damage occurred in the hoof crown, especially hoof fork and causes hooves
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