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SN/T 2584-2010: Protocol of real time polymerase chain reaction for detecting genetically modified components in rice and its derived products
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2584-2010 | English | 259 |
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Protocol of real time polymerase chain reaction for detecting genetically modified components in rice and its derived products
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SN/T 2584-2010
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Basic data | Standard ID | SN/T 2584-2010 (SN/T2584-2010) | | Description (Translated English) | Protocol of real time polymerase chain reaction for detecting genetically modified components in rice and its derived products | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X11 | | Classification of International Standard | 67.060 | | Word Count Estimation | 10,120 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | GB 6682; SN/T 1193; SN/T 1194 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the rice and rice products in GMO detection methods. This standard applies to transgenic Bt rice (TT51-1, Kemingdao, �� excellent Kefeng the 6th) and herbicide resistant transgenic rice (LLRICE62, LLRICE601) detection, but also for GMO detection in rice products. | SN/T 2584-2010: Protocol of real time polymerase chain reaction for detecting genetically modified components in rice and its derived products
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Protocol of real time polymerase chain reaction for detecting genetically modified components in rice and its derived products
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Genetically Modified Rice and Its Products
Real-time PCR detection method
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China
And Beijing, China Entry-Exit Inspection and Quarantine Hubei People's Republic of China.
The main drafters of this standard. Chen Yun, Liang Miao, Chen Shuangya, Wang Zhenhua, Li Ling, Huang Wensheng, Hu small bell, water Zhu Fang, Chen Hongjun.
Genetically Modified Rice and Its Products
Real-time PCR detection method
1 Scope
This standard specifies the method for the detection of rice and rice products genetically modified ingredients.
This standard applies to transgenic Bt rice (TT51-1, Kemingdao, Ⅱ excellent Kefeng 6) and herbicide-tolerant transgenic rice
(LLRICE62, LLRICE601) detection, but also for the detection of genetically modified rice products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
GB 6682 analytical laboratory use specifications and test methods
SN/T 1193 genetic testing laboratory technical requirements
Detection sampling and sample preparation method SN/T 1194 in genetically modified plants and their products
3 Abbreviations
The following abbreviations apply to this document.
3.1
crylAc gene crylAcgene
Encoding Bacillus thuringiensis (Bacillusthuringiensis) gene CrylAc insecticidal crystal protein.
3.2
crylAb gene crylAbgene
Encoding Bacillus thuringiensis (Bacillusthuringiensis) gene CrylAb insecticidal crystal protein.
3.3
crylAb/crylAc fusion gene crylAb/crylAcfusiongene
crylAb artificial gene and gene splicing crylAc formation genes.
3.4
sps gene sucrosephosphatesynthase
Sucrose phosphate synthase gene, the rice used in this standard within the standard gene.
3.5
gos9 gene gosgene
The roots of a rice gene expression in this standard is used as internal standard gene in rice.
3.6
PLD gene phospholipaseDgene
Phospholipase D gene.
3.7
Ct values cyclethreshold
When the number of cycles for each reaction tube fluorescent signal reaches the set threshold experienced by some brands of quantitative PCR instrument, also known as Cp
(Crosspoint) value.
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