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Microbiological examination method for milk and milk products hygiene. Part 5: Detection of Salmonella spp
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SN/T 2552.5-2010
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Basic data | Standard ID | SN/T 2552.5-2010 (SN/T2552.5-2010) | | Description (Translated English) | Microbiological examination method for milk and milk products hygiene. Part 5: Detection of Salmonella spp | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 14,138 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | SN/T 2552.1; SN/T 2552.2 | | Adopted Standard | ISO 6785-2001, MOD | | Regulation (derived from) | National Quality Inspection [2010] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies test methods of Salmonella in milk powder. This standard applies to testing of Salmonella in milk, other dairy products may refer to the use. | SN/T 2552.5-2010: Microbiological examination method for milk and milk products hygiene. Part 5: Detection of Salmonella spp
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Microbiological examination method for milk and milk products hygiene.Part 5. Detection of Salmonella spp
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Milk and dairy hygiene microbiology test methods
Part 5. Salmonella test
Part 5. DetectionofSalmonellaspp.
(ISO 6785.2001, Milkandmilkproducts-DetectionofSalmonellaspp, MOD.)
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2552 "Milk and dairy products Hygienic science test methods" is divided into thirteen sections.
--- Part 1. Guidance on sampling;
--- Part 2. Preparation of test sample dilution;
--- Part 3. yeast, mold colony count;
--- Part 4. Psychrophile microbial colony counts;
--- Part 5. Salmonella testing;
--- Part 6. Citrobacter inspection;
--- Part 7. Enterobacter cloacae inspection;
--- Part 8. Proteus mirabilis and inspection;
--- Part 9. Klebsiella test;
--- Part 10. Enterobacter sakazakii immunofluorescence test;
--- Part 11. Bacillus cereus Isolation and counting;
--- Part 12. monocytogenes Listeria detection and counting;
--- Part 13. Pseudomonas Isolation and counting.
This section is SN/T 2552 Part 5.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The partial modification using ISO 6785. ". Milkandmilkproducts-DetectionofSalmonellaspp" 2001, with its
The main differences are as follows.
--- According to GB/T 1.1 standard and some formatting Chinese customs were modified.
--- Some of the statements apply to international standards to apply to our standard formulation.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This part of the Chinese Academy of Inspection and Quarantine is responsible for drafting.
The main drafters of this section. Zhaogui Ming, Liu, Li Jin, in Huan, Zhao Yongsheng, Liu Pei, Yang Hairong, Fei Yuan.
Milk and dairy hygiene microbiology test methods
Part 5. Salmonella test
1 Scope
This section SN/T 2552 provisions of the milk powder Salmonella test.
This section applies to milk powder Salmonella test, you can refer to the use of other dairy products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
SN/T 2552.1 milk and dairy hygiene microbiology test methods - Part 1. Guidance on sampling
SN/T 2552.2 milk and dairy hygiene Microbiological test method - Part 2. Preparation of test sample dilution
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Salmonella Salmonellaspp.
On selective plates inoculated growth, and it complies with the typical colonies Salmonella biochemical and serological characteristics of microorganisms.
3.2
Salmonella chromogenic medium ChromogenicSalmonellaMedium
A method according C8 esterase producing Salmonella specific enzyme or other characteristics, added to the medium in appropriate chromogenic substrate, Salmonella
Growth decomposition of colonies of the substrate so that it appeared a class of unique color selective media.
4 Equipment and Materials
4.1 Balance. a sense of the amount of 0.1g.
4.2 homogenizer. slap or peristaltic type.
4.3 Incubator. 37 ℃ ± 1 ℃.
4.4 autoclaving pot. 115 ℃ ~ 121 ℃.
4.5 Water bath. 41.5 ℃ ± 1 ℃, 37.5 ℃ ± 1 ℃, 55 ℃ ± 1 ℃.
4.6 pH meter. 25 ℃ measurement accuracy of ± 0.1pH units.
4.7 scale straw or automatic pipette. Volume 1mL and 10mL, indexing were 0.5mL and 0.1mL.
4.8 sterile petri dish. diameter 90mm or 100mm.
4.9 cylinder.
5 medium and reagents
5.1 buffered peptone water. See Appendix A A.1 chapter.
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