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SN/T 2552.11-2010: Microbiological examination for milk and milk products hygiene. Part 11: Isolation and enumeration of Bacillus cereus
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| SN/T 2552.11-2010 | English | 369 |
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Microbiological examination for milk and milk products hygiene. Part 11: Isolation and enumeration of Bacillus cereus
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SN/T 2552.11-2010
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Basic data | Standard ID | SN/T 2552.11-2010 (SN/T2552.11-2010) | | Description (Translated English) | Microbiological examination for milk and milk products hygiene. Part 11: Isolation and enumeration of Bacillus cereus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 14,163 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | SN/T 2552.1; SN/T 2552.2; SN/T 1538.2-2007 | | Adopted Standard | ISO 7932, NEQ | | Regulation (derived from) | National Quality Inspection [2010] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method for separating and counting milk and dairy products of Bacillus cereus. This standard applies to milk and dairy products Bacillus cereus isolated and counted. | SN/T 2552.11-2010: Microbiological examination for milk and milk products hygiene. Part 11: Isolation and enumeration of Bacillus cereus
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Microbiological examination for milk and milk products hygiene.Part 11. Isolation and enumeration of Bacillus cereus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Milk and dairy hygiene microbiology test methods
Part 11. Bacillus cereus Isolation and counting
Part 11. IsolationandenumerationofBacilluscereus
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2552 "Milk and dairy products Hygienic science test methods" is divided into thirteen sections.
--- Part 1. Guidance on sampling;
--- Part 2. Preparation of test sample dilution;
--- Part 3. yeast, mold colony count;
--- Part 4. Psychrophile microbial colony counts;
--- Part 5. Salmonella testing;
--- Part 6. Citrobacter inspection;
--- Part 7. Enterobacter cloacae inspection;
--- Part 8. Proteus mirabilis and inspection;
--- Part 9. Klebsiella test;
--- Part 10. Enterobacter sakazakii immunofluorescence test;
--- Part 11. Bacillus cereus Isolation and counting;
--- Part 12. monocytogenes Listeria detection and counting;
--- Part 13. Pseudomonas isolation count.
This section is SN/T 2552 Part 11.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The section referring to the ISO 7932 "Microbiology of food and animal feed suspected of Bacillus cereus count level method 30 ℃ colonies
Counting technique "(ISO 7932 Microbiologyoffoodandanimalfeedingstuffs-Horizontalmethodforthee-
numerationofpresumptiveBacilluscereus-Colony-counttechniqueat30degreesC) and the US FDA "fine
Analysis Handbook Chapter 14 bacteria Bacillus cereus "(BacteriologicalAnalyticalManual-chapter14Bacilluscereus).
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Jilin Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine.
The main drafters of this section. Wang Zhenguo, Luo Yan Fei, Cai Yang, Zhaogui Ming, Liu Jinhua.
Milk and dairy hygiene microbiology test methods
Part 11. Bacillus cereus Isolation and counting
1 Scope
This section SN/T 2552 specifies the method for separating and counting milk and dairy products of Bacillus cereus.
This section applies to the separation and counting milk and dairy products of Bacillus cereus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
SN/T 2552.1 milk and dairy hygiene microbiology test methods - Part 1. Guidance on sampling
SN/T 2552.2 milk and dairy hygiene Microbiological test method - Part 2. Preparation of test sample dilution
SN/T 1538.2-2007 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Bacillus cereus Bacilluscereus
One kind of aerobic gram-positive bacillus, commonly found in soil, vegetables and raw products and cooked food products, the ability to propagate, it is leading to
Food poisoning pathogenic microorganisms.
Principle 4
4.1 use of Bacillus cereus and does not ferment mannitol produce characteristic lecithin, forming a typical colonies on plates were MYP
Separation.
4.2 Hemolytic use Bacillus cereus inoculated sheep blood agar plates for secondary screening.
4.3 The preliminary biochemical confirmation of suspected colonies after purification, to identify Bacillus cereus group.
4.4 biochemical identification by the group, identified Bacillus cereus species.
4.5 according to the number of confirmed positive for Bacillus cereus per gram of Bacillus cereus number or per milliliter sample.
5 medium and reagents
5.1 mannitol yolk polymyxin B Agar (MYP). See Appendix A A.1 chapter.
5.2 sheep blood agar. See Appendix A, Section A.2 chapter.
5.3 nutrient agar (NA). See Appendix A A.3 chapter.
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