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SN/T 2552.4-2010: Microbiological examination of milk and milk products hygiene. Part 4: Colony-count method of psychrotrophic microorganisms
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Microbiological examination of milk and milk products hygiene. Part 4: Colony-count method of psychrotrophic microorganisms
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SN/T 2552.4-2010
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Basic data | Standard ID | SN/T 2552.4-2010 (SN/T2552.4-2010) | | Description (Translated English) | Microbiological examination of milk and milk products hygiene. Part 4: Colony-count method of psychrotrophic microorganisms | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 18,125 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | SN/T 2552.1; SN/T 255.2 | | Adopted Standard | ISO 6730-2005/IDF 101, MOD; ISO 8552-2004/IDF 132, MOD | | Regulation (derived from) | National Quality Inspection [2010] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the 6. 5��C under the conditions of psychrotrophic microorganisms colony counting method, 2l��C under the conditions of psychrotrophic microorganisms assessment Zhuo quick way to count down. This standard applies to raw milk, pasteurized milk and other heat-treated milk psychrotrophic microorganism colony counts, milk and other dairy products may refer to the use. | SN/T 2552.4-2010: Microbiological examination of milk and milk products hygiene. Part 4: Colony-count method of psychrotrophic microorganisms
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Microbiological examination of milk and milk products hygiene.Part 4. Colony-count method of psychrotrophic microorganisms
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Milk and dairy hygiene microbiology test methods
Part 4. psychrophilic microbial colony counts
Part 4. Colony-countmethodofpsychrotrophicmicroorganisms
(ISO 6730.2005/IDF101, Milk-Enumerationofcolonyformingunitsof
psychrotrophicmicroorganisms-Colony-counttechniqueat6.5 ℃;
ISO 8552.2004/IDF132, Milk-Estimationofpsychrotrophic
microorganisms-Colony-counttechniqueat21 ℃, MOD)
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2552 "Milk and dairy products Hygienic science test methods" is divided into thirteen sections.
--- Part 1. Guidance on sampling;
--- Part 2. Preparation of test sample dilution;
--- Part 3. yeast, mold colony count;
--- Part 4. psychrophilic microbial colony counts;
--- Part 5. Salmonella testing;
--- Part 6. Citrobacter inspection;
--- Part 7. Enterobacter cloacae inspection;
--- Part 8. Proteus mirabilis and test methods;
--- Part 9. Klebsiella test;
--- Part 10. Enterobacter sakazakii immunofluorescence test methods;
--- Part 11. Bacillus cereus Isolation and counting;
--- Part 12. monocytogenes Listeria detection and counting;
--- Part 13. Pseudomonas Isolation and counting.
This section is SN/T 2552 Part 4.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This portion of the first modification method using ISO 6730.2005/IDF101 "psychrotrophic milk microbial colony counts 6.5 ℃ colony count technique"
(Milk-Enumeration ofcolonyforming unitsofpsychrotrophic microorganisms-Colony-count
techniqueat6.5 ℃), use the second method to modify the ISO 8552.2004/IDF132 assessment "milk psychrotrophic microorganism colony count of 21 ℃
Number of technology (Rapid method) "(Milk-Estimationofpsychrotrophicmicroorganisms-Colony-counttechniqueat
21 ℃), the main differences are as follows.
--- According to GB/T 1.1 standard and the Chinese habit for some formatting has been modified;
--- Some expression to international standards applicable to our standard way of expression;
--- Preamble has been modified;
--- Normative references cited the adoption of international standards in this series of standards, rather than international standards;
--- In order to practice more in line with our habits and requirements 5.2.4 will not use the scale straw volume requirements 11mL ± 0.2mL.
5.2.6 colony counting device will be modified as optional equipment;
--- In order to simplify the preparation of the medium, the first method and the second method used for the second medium unity with media law, namely plate count
Milk agar (see Appendix A).
--- 8.5.7 In order to avoid the spread of colonies, precautionary measures should be taken only recommended to take "after solidification, covered with medium" approach,
Without also recommended method, "a drop of glycerol on a Petri dish cover filter paper" in;
--- Unity "repetitive" requirement, the second law "repeatability" was changed to refer to the first law of "repetitive."
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section drafted by. China Inspection and Quarantine Science Research Institute, People's Republic of China Shandong Exit Inspection and Quarantine, the People's Republic of China
State and Jiangsu CIQ.
The main drafters of this section. Jiang Yinghui, Ray quality text, Zhaogui Ming, protect sea room, Zhao Liqing, Jia Juntao, Zhu Suzhen, Li Zhengyi, Tang Jing,
Ma Weixing, Zhang Jian, LIU Yun-guo, Zhu Changqing.
Milk and dairy hygiene microbiology test methods
Part 4. psychrophilic microbial colony counts
1 Scope
This section SN/T 2552 provisions under the colony counting method psychrotrophic microorganisms under conditions of 6.5 ℃, 21 ℃ condition of psychrotrophic microorganisms
To assess the number of colony counting fast method.
This section applies to raw milk, pasteurized milk and other heat-treated milk psychrophilic microbial colony counting, milk and dairy products to other
Referring use.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
SN/T 2552.1 milk and dairy hygiene microbiology test methods - Part 1. Guidance on sampling
SN/T 2552.2 milk and dairy hygiene Microbiological test method - Part 2. Preparation of test sample dilution
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Psychrophilic microorganisms psychrotrophicmicroorganisms
10d cultured under conditions specified in this 6.5 ℃, the formation of countable colonies of bacteria, yeasts and molds.
Principle 4
By plate count milk agar and a predetermined number of sample preparation pour plates. The test samples were tenfold dilutions, the same
Under the conditions of the preparation of other dilution plate. Plates under aerobic conditions, 6.5 ℃ cultured 10d, 21 ℃ culture 25h. To obtain satisfactory
Results, select the appropriate level of dilution to dilution levels and the number of colonies on agar plates obtained from the calculated per milliliter sample colony forming
Units (CFU).
This section includes the first normal 6.5 ℃ colony counting method and the second method 21 ℃ colony counting method (rapid method). Rapid second method described
Method is an approximate method. This is because under culture conditions of 21 ℃, except Psychrophile also other bacteria can grow. However, a large number of
The results showed that the first method and the resulting second method showed good correlation. The first method meet Psychrophile count more accurate requirements.
5 Equipment and Materials
5.1 General
5.1.1 SN/T 2552.2 requirements for all contact with the test sample, diluent and diluted sample or culture medium of the apparatus for uniform
Sterilization.
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