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Microbiological examination for milk and milk products hygiene. Part 3: Colony-count method of yeast and moulds
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SN/T 2552.3-2010
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Basic data | Standard ID | SN/T 2552.3-2010 (SN/T2552.3-2010) | | Description (Translated English) | Microbiological examination for milk and milk products hygiene. Part 3: Colony-count method of yeast and moulds | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 18,146 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Quoted Standard | SN/T 2552.1; SN/T 2552.2 | | Adopted Standard | ISO 6611-2004, MOD | | Regulation (derived from) | National Quality Inspection [2010] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the conditions at 25��C detection and counting in milk and dairy yeasts, molds seedlings colony forming units approach. This standard applies to milk, liquid milk, powdered milk, dried sweet whey, dried cheese, milk, lactose, cheese, acid casein, lactic casein, rennet casein, acid whey powder, butter, frozen dairy products (including frozen foods), cream puddings, desserts, fermented milk and cream kind of yeast, mold counts. This section does not apply to fresh cheese in yeast detection. | SN/T 2552.3-2010: Microbiological examination for milk and milk products hygiene. Part 3: Colony-count method of yeast and moulds
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Microbiological examination for milk and milk products hygiene.Part 3. Colony-count method of yeast and moulds
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Milk and dairy hygiene microbiology test methods
Part 3. yeast, mold colony count
Part 3. Colony-countmethodofyeastandmoulds
(ISO 6611.2004, Milkandmilkproducts-
Colony-counttechniqueat25 ℃, MOD)
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2552 "Milk and dairy products Hygienic science test methods" is divided into thirteen sections.
--- Part 1. Guidance on sampling;
--- Part 2. Preparation of test sample dilution;
--- Part 3. yeast, mold colony count;
--- Part 4. Psychrophile microbial colony counts;
--- Part 5. Salmonella testing;
--- Part 6. Citrobacter inspection;
--- Part 7. Enterobacter cloacae inspection;
--- Part 8. Proteus mirabilis and inspection;
--- Part 9. Klebsiella test;
--- Part 10. Enterobacter sakazakii immunofluorescence test;
--- Part 11. Bacillus cereus Isolation and counting;
--- Part 12. monocytogenes Listeria detection and counting;
--- Part 13. Pseudomonas Isolation and counting.
This section is SN/T 2552 Part 3.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This part of ISO 6611.2004 "Milk and dairy products yeast, mold 25 ℃ colony forming units counting method" (ISO 6611.2004
Milkandmilkproducts-Enumerationofcolony-formingunitsofyeastsand/ormoulds-Colony-
counttechniqueat25 ℃) unanimously adopted a degree of modification, the main differences are as follows.
--- According to GB/T 1.1 standard and the Chinese habit for some formatting has been modified;
--- Some of the statements apply to international standards to apply to our standard formulation;
--- The content of "normative references" in accordance with national standards were revised;
--- Delete ISO 6611.2004 "defined."
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This part of the Chinese Academy of Inspection and Quarantine, People's Republic of China Shandong Exit Inspection and Quarantine, People's Republic of China Jiangsu
Exit Inspection and Quarantine is responsible for drafting.
The main drafters of this section. Paul Room Sea Ray quality text, Zhaogui Ming, Jiang Yinghui, Jia Juntao, Zhao Liqing, LIU Yun-guo, Yanxian Hui, Lee Ming,
Wang Jing, Han Qing, Zhu Changqing, Zhao Jun, Linxiu Guang, Zhang Xiumei.
Milk and dairy hygiene microbiology test methods
Part 3. yeast, mold colony count
1 Scope
This section SN/T 2552 provides for the detection and enumeration of milk and dairy products yeast, mold colony forming units at 25 ℃
Methods.
This section applies to milk, liquid milk, powdered milk, dried sweet whey powder, cheese and dried milk, lactose, cheese, acid casein, lactic cheese
Su, rennet casein, acid whey powder, butter, frozen dairy products (including frozen foods), cream pudding, desserts, fermented milk and cream yeast species
Mother mold count.
This section does not apply to fresh cheese yeast detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
SN/T 2552.1 milk and dairy hygiene microbiology test methods - Part 1. Guidance on sampling
SN/T 2552.2 milk and dairy hygiene Microbiological test method - Part 2. Preparation of test sample dilution
Principle 3
3.1 test sample if the initial product is a liquid or in other products in the suspended solids, using a predetermined selective medium needs and provisions
Amount to prepare pour plates.
Test samples or other samples tenfold dilutions suspension, under the same conditions to prepare pour plates.
3.2 tablet under aerobic conditions, 25 ℃ ± 1 ℃ culture 5d.
3.3 In order to obtain satisfactory results, select the appropriate level of dilution to dilution levels and the number of colonies on agar plates obtained from the calculated per gram
Product per ml or yeasts, molds colony forming units (CFU).
4 dilution and media
4.1 Diluent
Accordance with the provisions SN/T 2552.2 of.
Aliquot 4.2 dilution, sterilization and storage
Accordance with the provisions SN/T 2552.2 of.
4.3 yeast extract, glucose, oxytetracycline, agar
See Appendix A A.1 chapter.
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