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SN/T 2497.21-2010 English PDF

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SN/T 2497.21-2010: Test method of import and export dangerous chemicals. Part 21: Agarose gel electrophoresis
Status: Valid
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SN/T 2497.21-2010English139 Add to Cart 2 days [Need to translate] Test method of import and export dangerous chemicals. Part 21: Agarose gel electrophoresis Valid SN/T 2497.21-2010

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Basic data

Standard ID SN/T 2497.21-2010 (SN/T2497.21-2010)
Description (Translated English) Test method of import and export dangerous chemicals. Part 21: Agarose gel electrophoresis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard G09
Classification of International Standard 13.300
Word Count Estimation 5,517
Date of Issue 2010-03-02
Date of Implementation 2010-09-16
Regulation (derived from) National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the import and export of dangerous chemicals by agarose gel electrophoresis and definitions of terms and operating procedures. This standard applies to import and export of dangerous chemicals agarose gel electrophoresis assay.

SN/T 2497.21-2010: Test method of import and export dangerous chemicals. Part 21: Agarose gel electrophoresis


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of import and export dangerous chemicals.Part 21. Agarose gel electrophoresis Exit inspection and quarantine industry standard book People's Republic of China Import and export of dangerous chemicals - Test methods Part 21. agarose gel electrophoresis Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts. --- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test; --- Part 2. plaque-forming cells (PFC) test; --- Part 3. Large Zhuan reproduction test; --- Part 4. Saccharomyces cerevisiae mitotic recombination test; --- Part 5. testicular cells UDS test; --- Part 6. mammalian cells in vitro sister chromatid exchange test; --- Part 7. mouse ear swelling test; Part --- Article 8.  fossa lymph node assay; --- Part 9. Determination of serum hemolysin test; Part --- Article 10. T lymphocyte proliferation assay test; --- Part 11. germline mutation assay; --- Part 12. single cell gel electrophoresis test; --- Part 13. fluorescence in situ hybridization; Part --- Article 14. SDS- polyacrylamide gel electrophoresis; --- Part 15. PCR-SSCP experiment; Part --- Article 16. Western-Blot test; --- Part 17. Behavior of mammalian toxicology tests; --- Part 18. Detection of DNA adducts; --- Part 19. NorthernBlot experiment; --- Part 20. Bradford protein assay; --- Part 21. agarose gel electrophoresis; --- Part 22. Tm value determination method of DNA; --- Part 23. organelle separation experimental methods; --- Part 24. cellular immunity in vitro assay method; --- Part 25. humoral immune function tests; --- Part 26. macrophage function tests; --- Part 27. Flow cytometry of apoptosis; --- Part 28. Application shuttle plasmid mutations Inspection; --- Part 29. Biochemical Oxygen Demand (BOD) was measured. This is Part 21 SN/T 2497 series of standards. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau. The main drafters of this section. Lu Gang, Wangli Bing, Li Jing, Zhang Ying, Zhao Haolibao, Pang Zhen. The first part of the Department of Inspection and Quarantine issued by industry standards. Import and export of dangerous chemicals - Test methods Part 21. agarose gel electrophoresis

1 Scope

This section SN/T 2497 provisions of the import and export of hazardous chemicals by agarose gel electrophoresis and definitions of terms and procedures. This section applies to the import and export of hazardous chemicals by agarose gel electrophoresis.

2 Terms and definitions

The following terms and definitions apply to this section. 2.1 A simple and efficient method for isolation and purification of DNA fragments. Since both sides of the double helix DNA molecule containing a skeleton with a negative charge The phosphate residues and thus move to the cathode in an electric field. Under certain field intensity, the migration velocity depends on the DNA molecule sieve effect. DNA mobility rate has a different molecular weight is not the same, which can be based on the size of the DNA molecule to make it separate. During electrophoresis can be obtained by detecting the tracer dye or relative molecular mass and standard reference samples were electrophoresed together.

3 Reagents and materials

3.1 electrophoresis buffer. buffer can be appropriately selected according to Table 1 in accordance with the test requirements and the actual situation. Table 1 Common electrophoresis buffer Buffer stock solution (1L) Tris- acetate (TAE) 50 × 242gTris base, 57.1mL glacial acetic acid, 100mL0.5mol/LEDTA (pH 8.0) Tris- phosphate (TPE) 10 × 108gTris base, 15.5mL85% phosphoric acid, 40mL0.5mol/LEDTA (pH 8.0) Tris- borate (TBE) 5 × 54gTris base, 27.5g boric acid, 20mL0.5mol/LEDTA (pH 8.0) Basic buffer 1 × 5mL10mol/LNaOH, 2mL0.5mol/LEDTA (pH 8.0) Note. The basic buffer should now use the existing. 3.2 ethidium bromide solution. working solution 0.5μg/mL, stored. Ethidium bromide is a strong mutagen, must wear gloves when using. 3.3 Electrophoresis grade agarose. according to the size of DNA fragments separated by agarose gel of an appropriate concentration formulated in accordance with Table 2 selection. Table 2 content agarose gel separation range Effective separation range of agarose content /% linear DNA molecules (kb) 0.3 5 ~ 60 0.6 1 ~ 20 10 0.7 0.8 7 0.9 0.5 1.2 0.4 ~ 6 3 0.2 ~ 1.5 2 2.0 0.1 3.4 loading buffer.

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