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SN/T 2497.12-2010: Test method of import and export dangerous chemicals. Part 12: Single cell gel electrophoresis (SCGE) assay
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2497.12-2010 | English | 159 |
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Test method of import and export dangerous chemicals. Part 12: Single cell gel electrophoresis (SCGE) assay
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SN/T 2497.12-2010
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Basic data | Standard ID | SN/T 2497.12-2010 (SN/T2497.12-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 12: Single cell gel electrophoresis (SCGE) assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 6,621 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Quoted Standard | GB 14924.1; GB 14925 | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals single cell gel electrophoresis test methods, test procedures, test results and results reporting. This standard applies to import and export of dangerous chemicals single cell gel electrophoresis test. | SN/T 2497.12-2010: Test method of import and export dangerous chemicals. Part 12: Single cell gel electrophoresis (SCGE) assay
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Test method of import and export dangerous chemicals.Part 12. Single cell gel electrophoresis (SCGE) assay
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 12. single cell gel electrophoresis test
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This section SN/T 2497 series of standards Part 12.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Zhang Yuan, Wangli Bing, Wang Hua, Zhao Zhuo, Han Wei, Xiong Zhongjiang.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 12. single cell gel electrophoresis test
1 Scope
SN/T 2497 provisions of this part of the test methods for the import and export of hazardous chemicals single cell gel electrophoresis test, test step
Step, test results and report the results.
This section applies to the import and export of hazardous chemicals single cell gel electrophoresis test.
2 Normative references
The following documents contain provisions which, through reference SN/T 2497 to the present, constitute provisions of this section. Any documents dated, with its
After all of the amendments (not including errata content) or revisions do not apply to this section, however, encourage the parties to reach an agreement under this section
Research on whether to use the latest versions of these documents. Undated document, the latest versions apply to this section.
GB 14924.1 animal feed common quality standards
GB 14925 laboratory animal environment and implementation
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this section.
3.1.1
High salt and detergent to break the cell membrane to remove protein, is a DNA fragment under the influence of the electric field strength can be moved out from the nucleus.
3.1.2
DNA double helix in the liquid helicase action, double-stranded DNA denatured into single strands.
3.2 Acronyms
The following abbreviations apply to this section.
3.2.1
3.2.2
3.2.3
3.2.4
PBS phosphate buffered saline
3.2.5
Principle 4
DNA strand breaks in the molecule appears clastogen action, the mononuclear cells are embedded in agarose gels, cells were lysed reconciliation supination
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