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SN/T 2436-2010: Protocol of quarantine for Brucella in sheep and goat
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Protocol of quarantine for Brucella in sheep and goat
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SN/T 2436-2010
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Basic data | Standard ID | SN/T 2436-2010 (SN/T2436-2010) | | Description (Translated English) | Protocol of quarantine for Brucella in sheep and goat | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 24,295 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 6682; GB 16548 | | Adopted Standard | OIE(2008), MOD | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the goats and sheep brucellosis bengal plate agglutination test, tube agglutination test, complement fixation test, enzyme-linked immunosorbent assay, agar diffusion test and other technical requirements. This standard applies to goats and sheep cattle, sheep species, species of sheep brucellosis quarantine. | SN/T 2436-2010: Protocol of quarantine for Brucella in sheep and goat---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of quarantine for Brucella in sheep and goat
Exit inspection and quarantine industry standard book People's Republic of China
Goats and sheep brucellosis quarantine rules
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The standard reference OIE "Terrestrial animal diagnostic tests and vaccines Handbook" (2008 edition) 2.7.2 Chapter "goats and sheep brucellosis"
And Chapter 2.7.9 "Sheep brucellosis disease" Prepared.
The Standard Appendix F, Appendix G are normative appendices, Appendix A, Appendix B, Appendix C, Appendix D, Appendix E, Appendix H, Appendix I, attached
Information Appendix J is recorded.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Inner Mongolia People's Republic of China
Bureau, People's Republic of China Shandong CIQ.
The main drafters of this standard. Wang Yuling, JIANG, Zhao Linli, Yue Zhiqin, Zhao Xiangping, this Long Chen, Xiao Yan, Han Guang Ge, Liu Hongmei, Song Yuli.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Goats and sheep brucellosis quarantine rules
1 Scope
This standard specifies the goats and sheep brucellosis rose bengal plate agglutination test tube agglutination test, complement fixation test, enzyme-linked for Free
Immunosorbent test, agar diffusion test and other technical requirements.
This standard applies to goats and sheep cattle, sheep species, species of sheep brucellosis quarantine.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analysis laboratory specifications and test methods
GB 16548 diseases and diseases of animals and animal products processing biosafety regulations
3 Overview
See Appendix A.
4 clinical symptoms and pathological changes
See Appendix B.
5 is tested serum samples collected
5.1 blood collection and separation
By conventional methods and separating blood serum. Serum should be fresh, no protein coagulation, no hemolysis and no smell of corruption.
5.2 transport and storage
Serum to prevent freezing and heat, if not sent to the laboratory within 3d, serum required refrigerated transport method. Serum should be put to 4 ℃ refrigerator
Save spare.
6 laboratory tests
6.1 goats and sheep cattle, sheep species brucellosis
6.1.1.1 Reagents and materials
6.1.1.1.1 Antigen. use according to the instructions, use the front room temperature (22 ℃ ~ 25 ℃) 30min ~ 60min, and shake well.
6.1.1.1.2 Positive control serum. lyophilized before using the instructions dissolved in distilled water to a predetermined volume.
6.1.1.1.3 Negative control serum. lyophilized before using the instructions dissolved in distilled water to a predetermined volume.
6.1.1.1.4 glass. about 45cm × 30cm, requires clean, partitioned into 32 squares, the number of horizontal grid 8, wales four cells, each cell 4cm ×
4cm, each cell under 1cm wide and then draw a small grid, used to fill the number serum.
6.1.1.1.5 toothpick. flat round or similar small stick.
6.1.1.1.6 pipettes. lessons volume 25μL, 75μL.
6.1.1.2 Test Procedure
6.1.1.2.1 before the test serum should be set at room temperature (22 ℃ ~ 25 ℃) 30min ~ 60min.
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